Gene expression technology / edited by David V. Goeddel. - San Diego : Academic Press, ©1990. - 1 online resource (xxxi, 681 pages) : illustrations - Methods in enzymology, v. 185 0076-6879 ; . - Methods in enzymology ; v. 185. .

Includes bibliographical references and indexes.

Systems for heterologous gene expression / Expression of heterologous proteins in escherichia coli / Design and construction of expression plasmid vectors in escherichia coli / Promoter recognition and mRNA initiation by Escherichia coli E[sigma]70 / Use of Escherichia coli trp promoter for direct expression of proteins / Use of T7 RNA polymerase to direct expression of cloned genes / High-level translation initiation / Enhanced translational efficiency with two-cistron expression system / Sequences within ribosome binding site affecting messenger RNA translatability and method to direct ribosomes to single messenger RNA species / Vector for enhanced translation of foreign genes in Escherichia coli / Minimizing proteolysis in Escherichia coli: genetic solutions / Gene fusions for purpose of expression: An introduction / Fusions to staphylococcal protein A / Expression as trpE fusion / Engineering Escherchia coli to secrete heterologous gene products / Refolding of recombinant proteins / Expression of heterologous genes in Bacillus subtilis / Regulated promoter for high-level expression of heterologous genes in Bacillus subtilis / System for secretion of heterologous proteins in Bacillus subtilis / Inducible expression of regulatory genes in Bacillus subtilis / David V. Goeddel and David V. Goeddel -- Larry Gold and David V. Goeddel -- Paulina Balbas, Francisco Bolivar and David V. Goeddel -- Jay D. Gralla and David V. Goeddel -- Daniel G. Yansura, Dennis J. Henner and David V. Goeddel -- F. William Studier [and others] -- Larry Gold, Gary D. Stormo and David V. Goeddel -- Brigitte E. Schoner [and others] -- Herman A. De Boer, Anna S. Hui and David V. Goeddel -- Peter O. Olins, Shaukat H. Rangwala and David V. Goeddel -- Susan Gottesman and David V. Goeddel -- Mathias Uhlén, Tomas Moks and David V. Goeddel -- Björn Nilsson, Lars Abrahmsén and David V. Goeddel -- Daniel G. Yansura and David V. Goeddel -- Joan A. Stader, Thomas J. Silhavy and David V. Goeddel -- Tadahiko Kohno [and others] -- Dennis J. Henner and David V. Goeddel -- Stuart F.J. Le Grice and David V. Goeddel -- Vasantha Nagarajan and David V. Goeddel -- Dennis J. Henner and David V. Goeddel. Heterologous gene expression in yeast / Propagation and expression of cloned genes in yeast: 2-[mu]m circle-based vectors / Manipulating yeast genome using plasmid vectors / Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast / Expression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter / Induced expression using yeast copper metallothionein promoter / High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene / Glyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteins / Superimposition of temperature regulation on yeast promoters / Sequence and structural requirements for efficient translation in yeast / Vacuolar proteases in yeast Saccharomyces cerevisiae / Detection and inhibition of ubiquitin-dependent proteolysis / Contranslational amino-terminal processing / [alpha]-Factor leader-directed secretion of heterologous proteins from yeast / Use of heterologous and homologous signal sequences for secretion of heterologous proteins from yeast / Isolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects / Molecular and genetic approach to enhancing protein secretion / Scott D. Emr and David V. Goeddel -- Alan B. Rose, James R. Broach and David V. Goeddel -- Tim Stearns [and others] -- Lawrence M. Mylin [and others] -- Virginia L. Price [and others] -- Tina Etcheverry and David V. Goeddel -- S.M. Kingsman [and others] -- Steven Rosenberg [and others] -- Andrzej Z. Sledziewski [and others] -- Thomas F. Donahue, Mark A. Cigan and David V. Goeddel -- Elizabeth W. Jones and David V. Goeddel -- Keith D. Wilkinson and David V. Goeddel -- Richard L. Kendall [and others] -- Anthony J. Brake and David V. Goeddel -- Ronald A. Hitzeman [and others] -- Clinton E. Ballou and David V. Goeddel -- Vanessa Chisholm [and others]. Expression of heterologous genes in mammalian cells / Vectors used for expression in mammalian cells / Assembly of enhancers, promoters, and splice signals to control expression of transferred genes / Methods for introducing DNA into mammalian cells / Selection and coamplification of heterologous genes in mammalian cells / Optimizing cell and culture environment for production of recombinant proteins / Analysis of synthesis, processing, and secretion of proteins expressed in mammalian cells / Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil / Arthur D. Levinson and David V. Goeddel -- Randal J. Kaufman and David V. Goeddel -- Michael Kriegler and David V. Goeddel -- Wayne A. Keown [and others] -- Randal J. Kaufman and David V. Goeddel -- Jennie P. Mather and David V. Goeddel -- Andrew J. Dorner, Kaufman Randal J. and David V. Goeddel -- Bruce H. Horwitz, Daniel Dimaio and David V. Goeddel.

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The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.

Electronic reproduction.
[Place of publication not identified] :
HathiTrust Digital Library,

Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.


0121820866 9780121820862


Gene expression--Technique.
Molecular cloning.
Cloning, Molecular.
Gene Expression.
Genetic Techniques.
Expressivité génétique--Technologie.
Gene expression--Technique.
Molecular cloning.
Wetenschappelijke technieken.

Genetic engineering

Electronic books.

QP601 / .M49 v.185


QP601.M49 / v.185

W1 / ME9615K v.185 1990 QH 450 / G3262 1990

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