PCR in bioanalysis /

PCR in bioanalysis / edited by Stephen J. Meltzer. - Totowa, N.J. : Humana Press, ©1998. - 1 online resource (xi, 281 pages) - Methods in molecular biology ; v. 92 . - Methods in molecular biology (Clifton, N.J.) ; v. 92. .

Source of title; title from title screen.

Includes bibliographical references and index.

Application of nucleic acid amplification in clinical microbiology / Gorm Lisby -- Detection of rodent parvoviruses by PCR / David G. Besselsen -- The SURF technique : selective genetic analysis of microscopic tissue heterogeneity / Darryl Shibata -- Prenatal and presymptomatic diagnosis of Marfan syndrome using fluorescence PCR and an automated sequencer / Mei Wang, Maurice Godfrey -- Measurement of TNF and iNOS mRNA Using cDNA-equalized reverse transcriptase PCR / Jay K. Kolls, Jianming Xie -- PCR diagnosis of the bovine immunodeficiency-like virus / David L. Suarez, Cecelia A. Whetstone -- PCR analysis for the identification of porcine reproductive and respiratory syndrome virus in boar semen / Jane Christopher-Hennings, Eric A. Nelson -- Quantitative and discriminative detection of individual HIV-1 mRNA subspecies by an RNase mapping assay / Catherine Mary, Hideo Akaoka, Bernard Verrier -- Detection of the heat-labile toxin coding gene (LT-gene) of enterotoxigenic Escherichia coli / Séamus Fanning, Deirdre O'Meara, Lesley Cotter, John O'Mullane, Bartley Cryan -- Motif-dependent polymerase chain reaction (PCR) : DNA fingerprinting enterotoxigenic Escherichia coli / Séamus Fanning, Deirdre O'Meara, Lesley Cotter, Paddy Greer, Bartley Cryan -- In vitro transcription/translation analysis for the identification of translation-terminating mutations / Michael C. Luce, Cameron G. Binnie, Lauren N. Kam-Morgan, Matthew C. Cayouette -- A single-tube nested RT-PCR for the detection of Ross River virus / Loryn Sellner -- Application of PCR to transgenic plants / Michael Wassenegger -- Detection of hepatitis C virus RNA by semiquantitative reverse-transcription PCR / Anderson S. Gaweco, David H. Thiel -- Detection of Legionella species in bronchial fluid by PCR / Gorm Lisby -- Quantitative measurement of mRNA expression by competitive RT-PCR / Joe O'Connell, Triona Goode, Fergus Shanahan -- PCR detection of Toxoplasma gondii in human fetal tissues / Tamás Tóth, István Sziller, Zoltán Papp -- Rapid diagnosis of pulmonary tuberculosis using Roche AMPLICOR mycobacterium tuberculosis PCR test / Richard F. D'Amato, Albert Miller -- The use of flow cytometry and RT-PCR in the detection of circulating PSA-positive cells in prostate cancer / Emma J. Fadlon, Freddie C. Hamdy -- Detection of Chlamydia trachomatis and Trichomonas vaginalis in the vaginal introitus, posterior vagina, and endocervix by polymerase chain reaction / Jan Jeremias, Vera Tolbert, Steven S. Witkin -- Detection and isolation of differentially expressed genes by PCR / John M. Abraham -- Detection of transgene integrants and homologous recombinants in mice by polymerase chain reaction / Kristin M. Abraham, Nancy S. Longo, Judith A. Hewitt -- Direct analysis for familial adenomatous polyposis mutations / Steven M. Powell -- PCR fingerprinting for detection of deleted or amplified sequences in human cancer / Takashi Kohno, Jun Yokota.

PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.

9781592595754 0896034976 9780896034976 1592595758

Polymerase chain reaction--Laboratory manuals.
Molecular diagnosis--Laboratory manuals.
DNA fingerprinting--Laboratory manuals.
Polymerase Chain Reaction--methods.
MEDICAL--Laboratory Medicine.
MEDICAL--Nursing--Assessment & Diagnosis.
DNA fingerprinting.
Molecular diagnosis.
Polymerase chain reaction.
Polymerase kettingreactie.
Wetenschappelijke technieken.

polymerase chain reaction diagnose diagnosis microbiologie microbiology geneeskunde medicine biologische technieken biological techniques protocollen protocols Molecular Biology (General) Moleculaire biologie (algemeen)

Electronic books.
Laboratory manuals.

RB43.8.P64 / P37 1998


QH506.M45 / v.92

W1 ME9616J v.92 1998 QH 450.3 P3475 1998

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