Membrane protein protocols : expression, purification, and characterization / edited by Barry S. Selinsky. - Totowa, N.J. : Humana Press, ©2003. - 1 online resource (xiv, 334 pages) : illustrations - Methods in molecular biology ; v. 228 . - Methods in molecular biology (Clifton, N.J.) ; v. 228. .

Includes bibliographical references and index.

Expression and purification of the amphipathic form of rabbit cytochrome b5 in escherichia coli / L. Waskell -- Dihydroorotate dehydrogenase of escherichia coli / K.F. Jensen and S. Larsen -- A general approach for heterologous membrane protein expression in escherichia coli: The uncoupling protein, ucp1, as an example / A.Z. Shaw and B. Miroux -- Expression of membrane-bound iron-sulfur proteins / D. Schneider, C.L. Schmidt and A. Seidler -- Heterologous expression of human scavenger receptor class b type i (sr-bi) in pichia pastoris / C.H. Han [and others] -- Expression of oligomeric amiloride-sensitive epithelial sodium channel in sf9 insect cells / U.S. Rao -- Functional expression of his-tagged rhodopsin in sf9 insect cells / G.J. Bosman [and others] -- Preparation of glycerol facilitator for protein structure and folding studies in solution / D. Manley and J.D. O'Neil -- Solubilization of chemokine receptors from cell membranes / R. Staudinger and J.C. Bandres -- A systematic approach for the solubilization of the integral membrane protein lysophospholipid: Acyl-coa acyltransferase (lat) / C. Kerkhoff and V. Kaever -- Purification of omp50, a minor porin of campylobacter jejuni / J.M. Bolla -- Purification of porins from mycobacterium smegmatis / C. Heinz, E. Roth and M. Niederweis -- Isolation of the melanocortin 5 receptor: From cdna sequence to isolating an integral membrane protein / B.L. Clarke -- Purification of mammalian serine palmitoyltransferase, a hetero-subunit enzyme for sphingolipid biosynthesis, by affinity-peptide chromatography / K. Hanada and M. Nishijima -- Purification of phosphatidylglycerophosphate synthase from cultured mammalian cells / K. Kawasaki and M. Nishijima -- Purification of pancreastatin receptor from rat liver membranes / V. Sanchez-Margalet, J. Santos-Alvarez and S. Diaz-Troya -- Purification, reconstitution, and functional characterization of zinc transporter from rat renal brush border membranes / R. Prasad -- Isolation of lipid raft-associated proteolipids / J. Millan [and others] -- Purification of membrane-bound catechol-o-methyltransferase / M.J. Bonifacio and P. Soares-da-Silva -- Purification and characterization of transporter proteins from human erythrocyte membrane / D.N. Wang, M.J. Lemieux and J.M. Boulter -- Purification of the human erythrocyte ps-stimulated mg2+-atpase: A putative ps flippase / J.V. Lyles [and others] -- Reconstitution and assay of biogenic membrane-derived phospholipid flippase activity in proteoliposomes / S.N. Gummadi [and others] -- Expression, purification, and reconstitution of rat liver carnitine palmitoyltransferase i / N.F. Brown -- Crystallization in lipidic cubic phases: A case study with bacteriorhodopsin / V.I. Gordeliy [and others] -- Optical biosensor assay using retroviral receptor pseudotypes / J. Rucker.

Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at major universities and research centers around the world describe in detail the key techniques that have proven successful in the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls. Timely and highly practical, Membrane Protein Protocols: Expression, Purification, and Characterization offers investigators proven methods to determine the three-dimensional structure of membrane receptors and transport proteins, as well as proven practical help in the discovery of new clinical targets for the design of novel pharmaceutical agents useful in treating cancer, bacterial and viral infection, and many metabolic diseases.

1588291243 9781588291240 9781592594009 159259400X

Membrane proteins--Laboratory manuals.
Membrane Proteins.
Protéines membranaires--Manuels de laboratoire.
SCIENCE--Life Sciences--Biochemistry.
Membrane proteins.
Wetenschappelijke technieken.

Laboratory Manual.
Electronic books.
Laboratory manuals.

QP552.M44 / M433 2003


QH506 / .M45 v. 228

W1 / ME9616J v.228 2003 QU 25 / M5327 2003

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