Seidman, Shlomo.

Transgenic Xenopus : microinjection methods and developmental neurobiology / by Shlomo Seidman and Hermona Soreq. - Totowa, N.J. : Humana Press, ©1997. - 1 online resource (xviii, 198 pages) : illustrations - Neuromethods, 28 0893-2336 ; . - Neuromethods ; 28. .

Includes bibliographical references (pages 167-193) and index.

Preface to the Series -- Preface -- Acknowledgments -- Contents -- List of Color Plates -- Chapter 1 Scientific Background -- Chapter 2 Experimental Methodologies -- Chapter 3 Experimental Applications Human Acetylcholinesterase as a Model Nervous System Protein -- Chapter 4 Conclusions -- Appendices to Experimental Methodologies -- Appendix I Worldwide Xenopus Suppliers African Xenopus Facility -- Appendix II Laboratory Maintenance of Xenopus he & s Frogs Housing -- Appendix III Preparation of Buffers/Reagents for Microinjections Modified Barth�s Medium Appendix IV Microinjection EquipmentAppendix V In Vitro Fertilization and Microinjection of Xenopus Embryos -- Appendix VI Whole-Mount Staining for Catalytically Active Acetylcholinesterase -- Appendix VII Isolation of Xenopus Oocytes Using Collagenase -- References -- Index

The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access, at the ear- est stages, to the genomic basis underlying the system's ultimate complexity, as exhibited once its structures are fully formed. Yet, nerve cells are anatomically, physiolo- cally, and biochemically diverse. The multitude of d- tinctly different routes for their development thus makes the developing nervous system especially intriguing for molecular neurobiologists. In particular, the demands of modern molecular neuroscience call for the establishment of efficient yet versatile systems for studying these c- plex processes. Transgenic embryos of the frog Xenopus laevis offer an excellent system for approaching neuroscientific issues. Insertion of foreign genes is performed simply, by mic- injection under binocular observation; hundreds of in vitro-fertilized embryos can be microinjected in one experiment. Embryos develop in tap water, at room t- perature, and within a few days become independent swimming tadpoles with fully functioning neuromus- lar systems. Being relatively small, these organisms are amenable to detailed analyses at the levels of mRNA, protein, and cell. Their rapid development permits the study of morphogenetic processes involved in early development, such as myogenesis and neural induction, as well as those involved in organogenesis and formation of the brain, the musculature, and the interconnections between them. Foreign DNA remains predominantly extrachromosomal.

9781592596331 1592596339

10.1385/0896034577 doi


Xenopus laevis.
Frogs--Genetics.
Developmental neurobiology.
Xenopus--embryology.
Animals, Genetically Modified--embryology.
Neurobiology--methods.
Developmental neurobiology.
Frogs--Genetics.
Xenopus laevis.


Electronic books.

QL668.E265 / S45 1997

591.3/5

W1 / NE337G v.28 1997 QY 60.A6 / S458t 1997

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