Mammalian cell viability : methods and protocols / edited by Martin J. Stoddart. - New York : Springer, ©2011. - 1 online resource (xi, 240 pages) : illustrations (some color) - Methods in molecular biology, 740 1940-6029 ; Springer protocols . - Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 740. .

Includes bibliographical references and index.

Cell viability assays : introduction / Cell viability analysis using trypan blue : manual and automated methods / Estimation of cell number based on metabolic activity : the MTT reduction assay / WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells / Assessment of cell proliferation with resazurin-based fluorescent dye / xCELLigence system for real-time and label-free monitoring of cell viability / Analysis of tumor and endothelial cell viability and survival using sulforhodamine B and clonogenic assays / Annexin V/7-AAD staining in keratinocytes / Measurement of caspase activity : from cell populations to individual cells / Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes / Multiplexing cell viability assays / Cytotoxicity testing : measuring viable cells, dead cells, and detecting mechanism of cell death / Fluorescein diacetate for determination of cell viability in 3D fibroblast-collagen-GAG constructs / Confocal imaging protocols for live/dead staining in three-dimensional carriers / Viability assessment of osteocytes using histological lactate dehydrogenase activity staining on human cancellous bone sections / Measuring glutamate receptor activation-induced apoptotic cell death in ischemic rat retina using the TUNEL assay / Exploiting the liberation of Zn2+ to measure cell viability / Noninvasive bioluminescent quantification of viable stem cells in engineered constructs / Raman micro-spectroscopy as a non-invasive cell viability test / Closed ampoule isothermal microcalorimetry for continuous real-time detection and evaluation of cultured mammalian cell activity and responses / Digital image processing of live/dead staining / Martin J. Stoddart -- Kristine S. Louis and Andre C. Siegel -- Laszlo Kupcsik -- Martin J. Stoddart -- Ewa M. Czekanska -- Ning Ke [and others] -- Caroline Woolston and Stewart Martin -- Maya Zimmermann and Norbert Meyer -- Gabriela Paroni and Claudio Brancolini -- Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz -- Helga H.J. Gerets, Stephane Dhalluin, and Franck A. Atienzar -- Terry L. Riss, Richard A. Moravec, and Andrew L. Niles -- Heather M. Powell, Alexis D. Armour, and Steven T. Boyce -- Benjamin Gantenbein-Ritter . [and others] -- Katharina Jahn and Martin J. Stoddart -- Won-Kyu Ju and Keun-Young Kim -- Christian J. Stork and Yang V. Li -- Karim Oudina, Adeline Cambon-Binder, and Delphine Logeart-Avramoglou -- Sophie Verrier, Alina Zoladek, and Ioan Notingher -- Olivier Braissant and Alma U. "Dan" Daniels -- Pieter Spaepen [and others].

9781617791086 1617791083

978-1-61779-107-9 Springer http://www.springerlink.com

015810862 Uk


Cells--Physiology.
Cells--Analysis.
Cells--Aging.
Cell death.
Mammals--Physiology.
Cell Survival.
Mammals--physiology.
Cell death.
Cells--Aging.
Cells--Analysis.
Mammals--Physiology.

celbiologie cellular biology celkweek cell culture levenswetenschappen life sciences Cellular Biology Celbiologie


Electronic books.

QH582.4 / .M36 2011

571.6

W1 / ME9616J v.740 2011 QU 25

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