Therapeutic oligonucleotides : methods and protocols / edited by John Goodchild. - New York : Humana Press : Springer, ©2011. - 1 online resource (xi, 340 pages) : illustrations - Methods in molecular biology, 764 1064-3745 ; Springer protocols . - Methods in molecular biology (Clifton, N.J.) ; v. 764. Springer protocols (Series) .

Includes bibliographical references and index.

Therapeutic oligonucleotides / Dinucleotides containing 3-S-phosphorothiolate linkages / 2-O,4-C-methyleneoxymethylene bridged nucleic acids (2,4-BNACOC) / Non-covalent peptide-based strategy for ex vivo and in vivo oligonucleotide delivery / Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides / Nanoparticle for tumor targeted delivery of oligomers / Light-directed delivery of nucleic acids / Antibody targeted siRNA delivery / Aptamer-drug conjugation for targeted tumor cell therapy / Five-step process for screening antisense compounds for efficacy: gene target IL-12Rb2 / Diverse small non-coding RNAs in RNA interference pathways / Quantification of siRNAs in vitro and in vivo / Optimization of transfection conditions and analysis of siRNA potency using real-time PCR / siRNA knockdown of gene expression in endothelial cells / Using RNA interference in Schistosoma mansoni / Performing the labeled microRNA pull-down (LAMP) assay system : an experimental approach for high-throughput identification of microRNA-target mRNAs / Synthesis, purification, and characterization of oligoribonucleotides that act as agonists of TLR7 and/or TLR8 / Synthesis, purification, and characterization of immune-modulatory oligodeoxynucleotides that act as agonists of toll-like receptor 9 / Surface plasmon resonance investigation of RNA aptamer-RNA ligand interactions / Practical considerations for analyzing antigene RNAs (agRNAs) : RNA immunoprecipitation of argonaute protein / Inhibition of human papillomavirus expression using DNAzymes / John Goodchild -- James W. Gaynor and Richard Cosstick -- Yoshiyuki Hari [and others] -- Laurence Crombez [and others] -- Samir El Andaloussi [and others] -- Xinrong Liu, Yi Wang, and Donald J. Hnatowich -- Sigurd Bøe [and others] -- Masoud M. Toloue and Lance P. Ford -- Michael J. Donovan [and others] -- Nikki B. Marshall, Laura L. Hauck, and Dan V. Mourich -- Liande Li and Yi Liu -- Angie Cheng, Alexander V. Vlassov, and Susan Magdaleno -- Angie Cheng, Susan Magdaleno, and Alexander V. Vlassov -- Emily Dennstedt and Brad Bryan -- Rita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly -- Ren-Jun Hsu and Huai-Jen Tsai -- Tao Lan and Ekambar R. Kandimalla -- Mallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla -- Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulme -- Jacob C. Schwartz and David R. Corey -- Maria Luisa Benitez-Hess, Pablo Reyes-Gutierrez, and Luis Marat Alvarez-Salas.

The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.


English.

9781617791888 1617791881

10.1007/978-1-61779-188-8 doi

015804810 Uk


Oligonucleotides--Therapeutic use.
Polynucleotides.
Nucleotides.
Nucleic Acids, Nucleotides, and Nucleosides
Chemicals and Drugs
Oligonucleotides.
Oligonucleotides--Therapeutic use.

levenswetenschappen life sciences biochemie biochemistry nucleic acids chemie chemistry Biology (General) Biologie (algemeen)


Electronic books.

QH345

572.8/633

W1 / ME9616J v.764 2011 QU 25

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