In vivo cellular imaging using fluorescent proteins : methods and protocols / edited by Robert M. Hoffman. - New York : Humana Press, ©2012. - 1 online resource (xiii, 269 pages) : illustrations (some color) - Methods in molecular biology, 872 1940-6029 ; . - Methods in molecular biology (Clifton, N.J.) ; v. 872. .

Includes bibliographical references and index.

Assessing Cancer Cell Migration and Metastatic Growth In Vivo in the Chick Embryo Using Fluorescence Intravital Imaging / The Use of Fluorescent Proteins for Intravital Imaging of Cancer Cell Invasion / High-Resolution In Vivo Imaging of Fluorescent Proteins Using Window Chamber Models / In Vivo Imaging of Pancreatic Cancer with Fluorescent Proteins in Mouse Models / Lentivirus-Based DsRed-2-Transfected Pancreatic Cancer Cells for Deep In Vivo Imaging of Metastatic Disease / Noninvasive and Real-Time Fluorescence Imaging of Peritoneal Metastasis in Nude Mice / Three-Dimensional In Vivo Imaging of Tumors Expressing Red Fluorescent Proteins / Real-Time Visualization and Characterization of Tumor Angiogenesis and Vascular Response to Anticancer Therapies / In Vivo Imaging of Human Cancer with Telomerase-Specific Replication-Selective Adenovirus / Real-Time Fluorescence Imaging of Abdominal, Pleural, and Lymphatic Metastases / Real-Time Imaging of Tumors Using Replication-Competent Light-Emitting Microorganisms / GFP-Transgenic Animals for In Vivo Imaging: Rats, Rabbits, and Pigs / The Use of Fluorescent Proteins for Developing Cancer-Specific Target Imaging Probes / In Vivo Imaging of the Developing Mouse Embryonic Vasculature / Screening Reef Corals for Novel GFP-Type Fluorescent Proteins by Confocal Imaging / What Does It Take to Improve Existing Fluorescent Proteins for In Vivo Imaging Applications? / In Vivo Imaging of Oligonucleotide Delivery / Subcellular Imaging In Vivo: The Next GFP Revolution / Hon Sing Leong, Ann F. Chambers, and John D. Lewis -- James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David Entenberg, Jeffrey Wyckoff, John Condeelis, and Jeffrey E. Segall -- Gregory M. Palmer, Andrew N. Fontanella, Siqing Shan, and Mark W. Dewhirst -- Michael Bouvet and Robert M. Hoffman -- Zeqian Yu, Jiahua Zhou, and Robert M. Hoffman -- Hayao Nakanishi, Seiji Ito, Makoto Matsui, Yuichi Ito, Kazunari Misawa, and Yasuhiro Kodera -- Alexander P. Savitsky, Irina G. Meerovich, Victoria V. Zherdeva, Lyaysan R. Arslanbaeva, Olga S. Burova, Darina V. Sokolova, Elena M. Treshchalina, Anatoly Yu Baryshnikov, Ilya I. Fiks, Anna G. Orlova, Michael S. Kleshnin, Ilya V. Turchin, and Alexander M. Sergeev -- H. Rosie Xing and Qingbei Zhang -- Toshiyoshi Fujiwara -- Susanne Carpenter and Yuman Fong -- Yong A. Yu, Stephanie Weibel, and Aladar A. Szalay -- Takashi Murakami and Eiji Kobayashi -- Thomas E. McCann, Nobuyuki Kosaka, Peter L. Choyke, and Hisataka Kobayashi -- Irina V. Larina and Mary E. Dickinson -- Anya Salih -- Marc Zimmer -- Fumitaka Takeshita, Ryou-u Takahashi, Jun Onodera, and Takahiro Ochiya -- Robert M. Hoffman.

The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.


9781617797972 1617797979 1617797960 9781617797965

10.1007/978-1-61779-797-2 doi

101591980 DNLM

Cancer--Imaging--Laboratory manuals.
Cancer cells--Laboratory manuals.
Publication Formats
Investigative Techniques
Diagnostic Imaging.
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Image Processing, Computer-Assisted.
Diagnostic Techniques and Procedures.
Computing Methodologies.
Publication Characteristics
Amino Acids, Peptides, and Proteins
Information Science.
Chemicals and Drugs
Imaging, Three-Dimensional.
Luminescent Proteins.
Laboratory Manuals
Cancer cells.

Medicine. Radiology, Medical. Medicine & Public Health. Imaging / Radiology. geneeskunde volksgezondheid public health afbeelden imagery radiologie radiology Medicine (General) Geneeskunde (algemeen)

Electronic books.
Laboratory manuals.

RC270.3.D53 / I5 2012


W1 / ME9616J v.872 2012 QU 25

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