SiRNA design : methods and protocols / edited by Debra J. Taxman. - New York : Humana Press : Springer [distributor], ©2013. - 1 online resource (xiii, 392 pages) : illustrations (some color) - Methods in molecular biology, 942 1940-6029 ; . - Methods in molecular biology (Clifton, N.J.) ; v. 942. .

Includes bibliographical references and index.

What parameters to consider and which software tools to use for target selection and molecular design of small interfering RNAs / Methods for selecting effective siRNA target sequences using a variety of statistical and analytical techniques / Designing functional siRNA with reduced off-target effects / Design and screening of siRNAs against highly structured RNA targets / Engineering small interfering RNAs by strategic chemical modification / Design, selection, and evaluation of highly specific and functional siRNA incorporating unlocked nucleobase analogs / Design, preparation and evaluation of asymmetric small interfering RNA for specific gene silencing in mammalian cells / Design of nuclease-resistant fork-like small interfering RNA (fsiRNA) / Designing dual-targeting siRNA duplexes having two active strands that combine siRNA and MicroRNA-like targeting / Strategies for designing and Validating immunostimulatory siRNAs / Designing efficient and specific endoribonuclease-prepared siRNAs / Short hairpin RNA-mediated gene silencing / Design of lentivirally expressed siRNAs / Bifunctional short hairpin RNA (bi-shRNA) : design and pathway to clinical application / Design and chemical modification of synthetic short shRNAs as potent RNAi triggers / Production and application of long dsRNA in mammalian cells / Design of RNAi reagents for invertebrate model organisms and human disease vectors / Construction of shRNA expression plasmids for silkworm cell lines using single-stranded DNA and Bst DNA polymerase / Designing effective amiRNA and multimeric amiRNA against plant viruses / Downregulation of plant genes with miRNA-induced gene silencing / Olga Matveeva -- Shigeru Takasaki -- Yuki Naito and Kumiko Ui-Tei -- Neda Nasheri, John Paul Pezacki, and Selena M. Sagan -- Jesper B. Bramsen and Jørgen Kjems -- Narendra Vaish and Pinky Agarwal -- Chanil Chang [and others] -- Elena L. Chernolovskaya and Marina A. Zenkova -- Pal Sætrom -- Michael P. Gantier -- Vineeth Surendranath [and others] -- Luke S. Lambeth and Craig A. Smith -- Ying Poi Liu and Ben Berkhout -- Donald D. Rao [and others] -- Anne Dallas and Brian H. Johnston -- Katerina Chalupnikova, Jana Nejepinska, and Petr Svoboda -- Thomas Horn and Michael Boutros -- Hiromitsu Tanaka -- Muhammad Fahim and Philip J. Larkin -- Felipe Fenselau de Felippes.

The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology series, this work contains the kind of detailed description and implementation advice that guarantees successful results. Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design.


English.

9781627031196 1627031197

10.1007/978-1-62703-119-6 doi

101596271 DNLM


Small interfering RNA.
Investigative Techniques
RNA, Small Untranslated.
RNA, Antisense.
Publication Formats
Genetic Techniques.
Epigenesis, Genetic.
RNA.
Publication Characteristics
Biology.
Antisense Elements (Genetics)
Gene Expression Regulation.
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Biological Science Disciplines.
Nucleic Acids, Nucleotides, and Nucleosides
RNA, Untranslated.
Genetic Phenomena.
Disciplines and Occupations
Chemicals and Drugs
Phenomena and Processes
Natural Science Disciplines.
RNA, Small Interfering.
Nucleic Acids.
Genetics.
Methods.
Gene Targeting.
Gene Silencing.
Laboratory Manuals
Small interfering RNA.

chemie chemistry biotechnologie biotechnology humane genetica human genetics Chemistry (General) Chemie (algemeen)


Laboratory Manual.
Electronic books.

QP623.5.S63

660.6

W1 / ME9616J v.942 2013 QU 25

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