Tandem repeats in genes, proteins, and disease : methods and protocols / edited by Danny M. Hatters, Anthony J. Hannan. - New York : Humana Press, ©2013. - 1 online resource (xi, 258 pages) : illustrations. - Methods in molecular biology, 1017 1940-6029 ; . - Methods in molecular biology (Clifton, N.J.) ; v. 1017. .

Includes bibliographical references and index.

Longitudinal imaging and analysis of neurons expressing polyglutamine-expanded proteins / Atomic force microscopy assays for evaluating polyglutamine aggregation in solution and on surfaces / Morphometric analysis of Huntington's disease neurodegeneration in Drosophila / Size analysis of polyglutamine protein aggregates using fluorescence detection in an analytical ultracentrifuge / Method for the incremental expansion of polyglutamine repeats in recombinant proteins / Pulse shape analysis (PulSA) to track protein translocalization in cells by flow cytometry : applications for polyglutamine aggregation / Characterizing social behavior in genetically targeted mouse models of brain disorders / PCR amplification and sequence analysis of GC-rich sequences : aristaless-related homeobox example / Challenges of "Sticky" co-immunoprecipitation : polyalanine tract protein-protein interactions / Molecular pathology of polyalanine expansion disorders : new perspectives from mouse models / Yeast as a platform to explore polyglutamine toxicity and aggregation / Immuno-based detection assays to quantify distinct mutant huntingtin conformations in biological samples / Modeling and analysis of repeat RNA toxicity in Drosophila / Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis / Kinetic analysis of aggregation data / Bioinformatics method for identifying Q/N-rich prion-like domains in proteins / Detecting soluble polyQ oligomers and investigating their impact on living cells using split-GFP / Cell biological approaches to investigate polyglutamine-expanded AR metabolism / Andrey S. Tsvetkov, D. Michael Ando, and Steven Finkbeiner -- Kathleen A. Burke and Justin Legleiter -- Wan Song [and others] -- Saskia Polling, Danny M. Hatters, and Yee-Foong Mok -- Amy L. Robertson and Stephen P. Bottomley -- Yasmin M. Ramdzan, Rebecca Wood, and Danny M. Hatters -- Emma L. Burrows and Anthony J. Hannan -- May H. Tan, Jozef Gécz, and Cheryl Shoubridge -- T.R. Mattiske [and others] -- James N. Hughes and Paul Q. Thomas -- Martin L. Duennwald -- Gregor P. Lotz and Andreas Weiss -- S.E. Samaraweera [and others] -- Mats Holmberg and Ellen A.A. Nollen -- Regina M. Murphy -- Eric D. Ross [and others] -- Patrick Lajoie and Erik Lee Snapp -- Lori J. Cooper and Diane E. Merry.

The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.


9781627034388 1627034382 1627034374 9781627034371

10.1007/978-1-62703-438-8 doi


101615399 DNLM 016490824 Uk

Nucleotides--Laboratory manuals.
Genetics--Laboratory manuals.
Publication Formats.
Repetitive Sequences, Nucleic Acid.
Genome Components.
Pathologic Processes.
Phenomena and Processes.
Pathological Conditions, Signs and Symptoms.
Base Sequence.
Publication Characteristics.
Biological Science Disciplines.
Natural Science Disciplines.
Genetic Structures.
Molecular Structure.
Disciplines and Occupations.
Biochemical Phenomena.
Chemical Phenomena.
Genetic Phenomena.
Tandem Repeat Sequences.
Laboratory Manuals.
Human Anatomy & Physiology.
Health & Biological Sciences.
Animal Biochemistry.

Medicine. Human genetics. Medical genetics. Biomedicine. Gene Function. biomedische wetenschappen moleculaire genetica molecular genetics humane genetica Medicine (General) Geneeskunde (algemeen)

Electronic books.
Laboratory manuals.

QP625.N89 / .T36 2013


W1 / ME9616J v.1017 2013 QU 500

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