Neural progenitor cells : methods and protocols / edited by Brent A. Reynolds, Loic P. Deleyrolle. - New York : Humana Press, ©2013. - 1 online resource (xi, 241 pages) : illustrations (some color) - Methods in Molecular Biology, 1059 1940-6029 ; . - Methods in molecular biology (Clifton, N.J.) ; v. 1059. .

Includes bibliographical references and index.

Culturing Fetal Precursor Cells Using Free Floating Serum-Free Conditions / In Vitro Expansion of Fetal Neural Progenitors as Adherent Cell Lines / Isolate and Culture Precursor Cells from the Adult Periventricular Area / Culturing and Expansion of Precursor Cells from the Adult Hippocampus / Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord / Culturing and Expansion of "Clinical Grade" Precursors Cells from the Fetal Human Central Nervous System / Isolating and Culturing of Precursor Cells from the Adult Human Brain / Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord / Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny / Isolation of Adult Stem Cells from the Human Olfactory Mucosa / Enumerating Stem Cell Frequency: Neural Colony Forming Cell Assay / Flow Cytometry of Neural Cells / Neonatal Transplant in Hypoxic Injury / Isolation and Purification of Self-Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke / Transplantation of Fetal Midbrain Dopamine Progenitors into a Rodent Model of Parkinson's Disease / Distribution of Neural Precursor Cells in the Adult Mouse Brain / Identifying Neural Progenitor Cells in the Adult Human Brain / Clinical Trials for the Treatment of Spinal Cord Injury: Not So Simple / Andrew Chojnacki and Samuel Weiss -- Steven M. Pollard -- Chiara Cavazzin, Margherita Neri, and Angela Gritti -- Dhanisha J. Jhaveri, Boris W. Prosper, and Perry F. Bartlett -- Jean-Philippe Hugnot -- Maurizio Gelati [and five others] -- Florian A. Siebzehnrubl and Dennis A. Steindler -- Luc Bauchet [and five others] -- Hassan Azari -- François Féron [and three others] -- Sharon A. Louis and Carmen K.H. Mak -- Geoffrey W. Osborne -- Tong Zheng and Michael D. Weiss -- Ricardo L. Azevedo-Pereira and Marcel M. Daadi -- Lachlan H. Thompson and Clare L. Parish -- Daniel G. Blackmore and Rodney L. Rietze -- Thomas I.H. Park [and seven others] -- Alan Mackay-Sim and François Féron.

"The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing "no new neuron" doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls."--


9781627035743 1627035745 1627035737 9781627035736

10.1007/978-1-62703-574-3 doi

Multipotent stem cells.
Publication Formats
Stem Cells.
Publication Characteristics
Multipotent Stem Cells.
Laboratory Manuals
Neural Stem Cells.
Multipotent stem cells.

stamcellen stem cells biomedische wetenschappen biomedicine neurowetenschap neuroscience neurobiologie neurobiology Medicine (General) Geneeskunde (algemeen)

Electronic books.



W1 / ME9616J v.1059 2013 WL 25

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