Cellular senescence : methods and protocols / edited by Marco Demaria. - 1 online resource (xii, 253 pages) : illustrations (some color) - Methods in molecular biology, volume 1896 1064-3745 ; . - Methods in molecular biology (Clifton, N.J.) ; v. 1896. .

Includes bibliographical references and index.

Detecting cellular senescence in reprogramming / DNA damage in situ ligation followed by proximity ligation assay (DI-PLA) / Reactive oxygen species detection in senescent cells / Cellular identification and quantification of senescence-associated ?-galactosidase activity in vivo / Relative human telomere length quantification by real-time PCR / Assessing functional roles of the senescence-associated secretory phenotype (SASP) / Measuring the inflammasome in oncogene-induced / Alarmin detection in senescent cells / IMR90 ER:RAS: a cell model of oncogene-induced senescence / Genotoxic stress-induced senescence / Multiparametric assay to evaluate senescent cells / Novel quantitative method for the detection of lipofuscin, the main by-product of cellular senescence, in fluids / Measurement of metabolite changes in senescent cells by mass spectrometry / Quantification of autophagy during senescence / Quantification of aneuploidy in mammalian systems / Identification of genomic alterations through multilevel DNA structural analysis / Mouse models of accelerated cellular senescence / Methods to quantify the NF-?B pathway during senescence / Coralie Cazin, Mathieu von Joest, and Han Li -- Alessandro Galbiati and Fabrizio d'Adda di Fagagna -- Stella Victorelli and Joa˜o F. Passos -- Bennett G. Childs, Tyler J. Bussian, and Darren J. Baker -- A. Vasilishina, A. Kropotov, I. Spivak, and A. Bernadotte -- Nicolas Malaquin, Véronique Tu, and Francis Rodier -- Irene Fernández-Duran, Nú ria Tarrats, Priya Hari, and Juan Carlos Acosta -- Dong Eun Kim and Albert R. Davalos -- Andrew J. Innes and Jesú s Gil -- Dorothy N. Y. Fan and Clemens A. Schmitt -- Hilah Gal, Ziv Porat, and Valery Krizhanovsky -- Sophia V. Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou, Panagiotis V. S. Vasileiou, Aggelos Margetis, Athanassios Kotsinas, Nikolaos G. Kastrinakis, Petros Sfikakis, Paul Townsend, Emmanuel Mikros, Constantinos Pantos, and Vassilis G. Gorgoulis -- Christopher D. Wiley, Sonnet Davis, and Arvind Ramanathan -- Joon Tae Park, Young-Sam Lee, and Sang Chul Park -- Hilda van den Bos, Bjorn Bakker, Aaron Taudt, Victor Guryev, Maria Colomé-Tatché, Peter M. Lansdorp, Floris Foijer, and Diana C. J. Spierings -- Ryan K. Shultzaberger and John Dresios -- Matthew J. Yousefzadeh, Kendra I. Melos, Luise Angelini, Christin E. Burd, Paul D. Robbins, and Laura J. Niedernhofer -- Lei Zhang, Jing Zhao, Aditi Gurkar, Laura J. Niedernhofer, and Paul D. Robbins.

9781493989317 1493989316


Cells--Aging--Laboratory manuals.
Cellular Senescence.
Cells--Aging.


Electronic books.
Laboratory Manual.
Laboratory manuals.

QH608

571.6

Powered by Koha