Laboratory methods in enzymology : (Record no. 200603)

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control field ocn865607940
003 - CONTROL NUMBER IDENTIFIER
control field OCoLC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20220204104524.0
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007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
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fixed length control field 131219s2013 ne a ob 001 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency BUF
Language of cataloging eng
Description conventions rda
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019 ## -
-- 1110420834
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
Canceled/invalid ISBN 9780124200678
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
Canceled/invalid ISBN 0124200672
029 1# - (OCLC)
OCLC library identifier NLGGC
System control number 370792084
029 1# - (OCLC)
OCLC library identifier DEBBG
System control number BV043877953
029 1# - (OCLC)
OCLC library identifier AU@
System control number 000052223956
035 ## - SYSTEM CONTROL NUMBER
System control number (OCoLC)865607940
Canceled/invalid control number (OCoLC)1110420834
050 #4 - LIBRARY OF CONGRESS CALL NUMBER
Classification number QP601
Item number .L32 2013eb
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 572/.7
Edition number 23
049 ## - LOCAL HOLDINGS (OCLC)
Holding library MAIN
245 00 - TITLE STATEMENT
Title Laboratory methods in enzymology :
Remainder of title cell, lipid and carbohydrate /
Statement of responsibility, etc. edited by Jon Lorsch.
246 30 - VARYING FORM OF TITLE
Title proper/short title Cell, lipid and carbohydrate
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture San Diego, CA :
Name of producer, publisher, distributor, manufacturer Academic Press,
Date of production, publication, distribution, manufacture, or copyright notice 2013.
264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Date of production, publication, distribution, manufacture, or copyright notice ©2013
300 ## - PHYSICAL DESCRIPTION
Extent 1 online resource (lix, 347 pages) :
Other physical details illustrations.
336 ## - CONTENT TYPE
Content type term text
Content type code txt
Source rdacontent
337 ## - MEDIA TYPE
Media type term computer
Media type code c
Source rdamedia
338 ## - CARRIER TYPE
Carrier type term online resource
Carrier type code cr
Source rdacarrier
347 ## - DIGITAL FILE CHARACTERISTICS
File type data file
Source rda
490 1# - SERIES STATEMENT
Series statement Methods in enzymology,
International Standard Serial Number 0076-6879 ;
Volume/sequential designation volume 533
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc Includes bibliographical references and index.
588 0# - SOURCE OF DESCRIPTION NOTE
Source of description note Print version record.
505 0# - FORMATTED CONTENTS NOTE
Formatted contents note Section I. Cell protocols : basic microbiological techniques. Pouring agar plates and streaking or spreading to isolate individual colonies / Elizabeth Eyler ; Storage of bacteria and yeast / Cheryl M. Koh -- Section II. Cell protocols : cellular fractionation. Cellular fractionation : mammalian cells / Joerg von Hagen and Uwe Michelsen ; Cellular fractionation : yeast cells / Joerg von Hagen and Uwe Michelsen -- Section III. Cell protocols : Knock-outs and gene replacements. Yeast-gene replacement using PCR products / Megan Bergkessel, Christine Guthrie, and John Abelson ; Measuring RNAi knockdown using qPCR / Stuart Milstein, Michael Nguyen, Rachel Meyers, and Antonin de Fougerolles ; Recombineering : using drug cassettes to knock out genes in vivo / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court ; Gene knockouts, in vivo site-directed mutagenesis and other modifications using the delitto perfetto system in Saccharomyces cerevisiae / Samantha Stuckey and Francesca Storici ; Gene targeting and site-specific recombination in mouse ES cells / Konstantinos Anastassiadis, Frank Schnütgen, Harald von Melchner, and A. Francis Stewart ; Recombineering : highly efficient in vivo genetic engineering using single-strand oligos / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court -- Section IV. Cell protocols : microbiological media. Growth media for E. coli / Juliane C. Lessard ; Saccharomyces cerevisiae growth media / Jessica S. Dymond -- Section V. Cell protocols : preparation of slides for microscopy. Immunohistochemistry on freely floating fixed tissue sections / Julia Bachman ; Preparation of slides for microscopy from frozen tissue sections / Julia Bachman ; Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry / Kirstie Canene-Adams ; Preparation of cells for microscopy using cytospin / Cheryl M. Koh ; Preparation of cells for microscopy using chamber slides and coverslips / Cheryl M. Koh ; Preparation of cells for microscopy using 'cell blocks' / Cheryl M. Koh -- Section VI. Lipid and carbohydrate protocols. Enzymatic deglycosylation of glycoproteins / Min-Sung Kim and Dan Leahy -- Section VII. Lipid and carbohydrate protocol : making vesicles and micelles. Preparation of fatty acid or phospholipid vesicles by thin-film rehydration / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Preparation of fatty acid micelles / Ting F. Zhu, Itay Budin, and Jack W. Szostak -- Section VIII. Other protocols. Reverse-phase HPLC analysis and purification of small molecules / Kirstie Canene-Adams ; Thin layer chromatography / Marina Santiago and Scott Strobel ; ATP and GTP hydrolysis assays (TLC) / Vaishnavi Rajagopal and Jon R. Lorsch.
520 8# - SUMMARY, ETC.
Summary, etc. These volumes of Methods in Enzymology contain the protocols that made up the on-line Methods Navigator. Our philosophy when we selected the protocols to include in the Navigator was that they should be for techniques useful in any biomedical laboratory, regardless of the system the lab studies. Each protocol was written by researchers who use the technique routinely, and in many cases by the people who actually developed the procedure in the first place. The protocols are very detailed and contain recipes for the necessary buffers and reagents, as well as flow-charts outlining the steps involved. Many of the chapters have accompanying videos demonstrating key parts of the procedures. The volumes are broken into distinct areas: DNA methods; Cell-based methods; lipid, carbohydrate and miscellaneous methods; RNA methods; protein methods. Our goal is that these protocols will be useful for everyone in the lab, from undergraduates and rotation students to seasoned post-doctoral fellows. We hope that these volumes will become dog-eared and well-worn in your laboratory, either physically or electronically.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Biotechnology
General subdivision Methodology.
9 (RLIN) 105751
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Molecular biology
General subdivision Methodology.
9 (RLIN) 81833
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Carbohydrates
Form subdivision Laboratory manuals.
9 (RLIN) 87486
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Cells
Form subdivision Laboratory manuals.
9 (RLIN) 93624
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Lipids
Form subdivision Laboratory manuals.
9 (RLIN) 965560
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Biotechnology
General subdivision Methodology.
Source of heading or term fast
Authority record control number (OCoLC)fst00832759
9 (RLIN) 105751
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Carbohydrates.
Source of heading or term fast
Authority record control number (OCoLC)fst00846754
9 (RLIN) 79896
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Cells.
Source of heading or term fast
Authority record control number (OCoLC)fst00850250
9 (RLIN) 2745
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Lipids.
Source of heading or term fast
Authority record control number (OCoLC)fst00999367
9 (RLIN) 84738
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Molecular biology
General subdivision Methodology.
Source of heading or term fast
Authority record control number (OCoLC)fst01024745
9 (RLIN) 81833
650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Biotechnology
General subdivision methods.
9 (RLIN) 21885
650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Carbohydrates
General subdivision analysis.
9 (RLIN) 82740
650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Molecular Biology
General subdivision methods.
9 (RLIN) 23201
655 #7 - INDEX TERM--GENRE/FORM
Genre/form data or focus term Laboratory manuals.
Source of term fast
Authority record control number (OCoLC)fst01920776
9 (RLIN) 965561
655 #7 - INDEX TERM--GENRE/FORM
Genre/form data or focus term Ebook.
Source of term local
9 (RLIN) 20044
655 #4 - INDEX TERM--GENRE/FORM
Genre/form data or focus term Ebook.
9 (RLIN) 20044
655 #4 - INDEX TERM--GENRE/FORM
Genre/form data or focus term Electronic books.
9 (RLIN) 396
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Lorsch, Jon,
Relator term editor.
9 (RLIN) 116868
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Print version:
Title Laboratory methods in enzymology.
Place, publisher, and date of publication Amsterdam, Netherlands ; Boston, Mass. : Elsevier/Academic Press Inc, ©2013
International Standard Book Number 9780124200678
Record control number (OCoLC)860394433
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title Methods in enzymology ;
Volume number/sequential designation v. 533.
International Standard Serial Number 0076-6879
9 (RLIN) 97896
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/00766879/533">https://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/00766879/533</a>
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