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06940cam a2200685Ii 4500 |
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ocn865607940 |
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OCoLC |
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20220204104524.0 |
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m o d |
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131219s2013 ne a ob 001 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
BUF |
| Language of cataloging |
eng |
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rda |
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pn |
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BUF |
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BUF |
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OPELS |
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| 019 ## - |
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| -- |
1110420834 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| Canceled/invalid ISBN |
9780124200678 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| Canceled/invalid ISBN |
0124200672 |
| 029 1# - (OCLC) |
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| OCLC library identifier |
NLGGC |
| System control number |
370792084 |
| 029 1# - (OCLC) |
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| OCLC library identifier |
DEBBG |
| System control number |
BV043877953 |
| 029 1# - (OCLC) |
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| OCLC library identifier |
AU@ |
| System control number |
000052223956 |
| 035 ## - SYSTEM CONTROL NUMBER |
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| System control number |
(OCoLC)865607940 |
| Canceled/invalid control number |
(OCoLC)1110420834 |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
QP601 |
| Item number |
.L32 2013eb |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
572/.7 |
| Edition number |
23 |
| 049 ## - LOCAL HOLDINGS (OCLC) |
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| Holding library |
MAIN |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Laboratory methods in enzymology : |
| Remainder of title |
cell, lipid and carbohydrate / |
| Statement of responsibility, etc. |
edited by Jon Lorsch. |
| 246 30 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
Cell, lipid and carbohydrate |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
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| Place of production, publication, distribution, manufacture |
San Diego, CA : |
| Name of producer, publisher, distributor, manufacturer |
Academic Press, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2013. |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Date of production, publication, distribution, manufacture, or copyright notice |
©2013 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
1 online resource (lix, 347 pages) : |
| Other physical details |
illustrations. |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
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| Media type term |
computer |
| Media type code |
c |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
online resource |
| Carrier type code |
cr |
| Source |
rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
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| File type |
data file |
| Source |
rda |
| 490 1# - SERIES STATEMENT |
|---|
| Series statement |
Methods in enzymology, |
| International Standard Serial Number |
0076-6879 ; |
| Volume/sequential designation |
volume 533 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc |
Includes bibliographical references and index. |
| 588 0# - SOURCE OF DESCRIPTION NOTE |
|---|
| Source of description note |
Print version record. |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Section I. Cell protocols : basic microbiological techniques. Pouring agar plates and streaking or spreading to isolate individual colonies / Elizabeth Eyler ; Storage of bacteria and yeast / Cheryl M. Koh -- Section II. Cell protocols : cellular fractionation. Cellular fractionation : mammalian cells / Joerg von Hagen and Uwe Michelsen ; Cellular fractionation : yeast cells / Joerg von Hagen and Uwe Michelsen -- Section III. Cell protocols : Knock-outs and gene replacements. Yeast-gene replacement using PCR products / Megan Bergkessel, Christine Guthrie, and John Abelson ; Measuring RNAi knockdown using qPCR / Stuart Milstein, Michael Nguyen, Rachel Meyers, and Antonin de Fougerolles ; Recombineering : using drug cassettes to knock out genes in vivo / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court ; Gene knockouts, in vivo site-directed mutagenesis and other modifications using the delitto perfetto system in Saccharomyces cerevisiae / Samantha Stuckey and Francesca Storici ; Gene targeting and site-specific recombination in mouse ES cells / Konstantinos Anastassiadis, Frank Schnütgen, Harald von Melchner, and A. Francis Stewart ; Recombineering : highly efficient in vivo genetic engineering using single-strand oligos / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court -- Section IV. Cell protocols : microbiological media. Growth media for E. coli / Juliane C. Lessard ; Saccharomyces cerevisiae growth media / Jessica S. Dymond -- Section V. Cell protocols : preparation of slides for microscopy. Immunohistochemistry on freely floating fixed tissue sections / Julia Bachman ; Preparation of slides for microscopy from frozen tissue sections / Julia Bachman ; Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry / Kirstie Canene-Adams ; Preparation of cells for microscopy using cytospin / Cheryl M. Koh ; Preparation of cells for microscopy using chamber slides and coverslips / Cheryl M. Koh ; Preparation of cells for microscopy using 'cell blocks' / Cheryl M. Koh -- Section VI. Lipid and carbohydrate protocols. Enzymatic deglycosylation of glycoproteins / Min-Sung Kim and Dan Leahy -- Section VII. Lipid and carbohydrate protocol : making vesicles and micelles. Preparation of fatty acid or phospholipid vesicles by thin-film rehydration / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Preparation of fatty acid micelles / Ting F. Zhu, Itay Budin, and Jack W. Szostak -- Section VIII. Other protocols. Reverse-phase HPLC analysis and purification of small molecules / Kirstie Canene-Adams ; Thin layer chromatography / Marina Santiago and Scott Strobel ; ATP and GTP hydrolysis assays (TLC) / Vaishnavi Rajagopal and Jon R. Lorsch. |
| 520 8# - SUMMARY, ETC. |
|---|
| Summary, etc. |
These volumes of Methods in Enzymology contain the protocols that made up the on-line Methods Navigator. Our philosophy when we selected the protocols to include in the Navigator was that they should be for techniques useful in any biomedical laboratory, regardless of the system the lab studies. Each protocol was written by researchers who use the technique routinely, and in many cases by the people who actually developed the procedure in the first place. The protocols are very detailed and contain recipes for the necessary buffers and reagents, as well as flow-charts outlining the steps involved. Many of the chapters have accompanying videos demonstrating key parts of the procedures. The volumes are broken into distinct areas: DNA methods; Cell-based methods; lipid, carbohydrate and miscellaneous methods; RNA methods; protein methods. Our goal is that these protocols will be useful for everyone in the lab, from undergraduates and rotation students to seasoned post-doctoral fellows. We hope that these volumes will become dog-eared and well-worn in your laboratory, either physically or electronically. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Biotechnology |
| General subdivision |
Methodology. |
| 9 (RLIN) |
105751 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular biology |
| General subdivision |
Methodology. |
| 9 (RLIN) |
81833 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Carbohydrates |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
87486 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cells |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
93624 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Lipids |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
965560 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Biotechnology |
| General subdivision |
Methodology. |
| Source of heading or term |
fast |
| Authority record control number |
(OCoLC)fst00832759 |
| 9 (RLIN) |
105751 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Carbohydrates. |
| Source of heading or term |
fast |
| Authority record control number |
(OCoLC)fst00846754 |
| 9 (RLIN) |
79896 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cells. |
| Source of heading or term |
fast |
| Authority record control number |
(OCoLC)fst00850250 |
| 9 (RLIN) |
2745 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Lipids. |
| Source of heading or term |
fast |
| Authority record control number |
(OCoLC)fst00999367 |
| 9 (RLIN) |
84738 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular biology |
| General subdivision |
Methodology. |
| Source of heading or term |
fast |
| Authority record control number |
(OCoLC)fst01024745 |
| 9 (RLIN) |
81833 |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Biotechnology |
| General subdivision |
methods. |
| 9 (RLIN) |
21885 |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Carbohydrates |
| General subdivision |
analysis. |
| 9 (RLIN) |
82740 |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular Biology |
| General subdivision |
methods. |
| 9 (RLIN) |
23201 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory manuals. |
| Source of term |
fast |
| Authority record control number |
(OCoLC)fst01920776 |
| 9 (RLIN) |
965561 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Ebook. |
| Source of term |
local |
| 9 (RLIN) |
20044 |
| 655 #4 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Ebook. |
| 9 (RLIN) |
20044 |
| 655 #4 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Electronic books. |
| 9 (RLIN) |
396 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lorsch, Jon, |
| Relator term |
editor. |
| 9 (RLIN) |
116868 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Relationship information |
Print version: |
| Title |
Laboratory methods in enzymology. |
| Place, publisher, and date of publication |
Amsterdam, Netherlands ; Boston, Mass. : Elsevier/Academic Press Inc, ©2013 |
| International Standard Book Number |
9780124200678 |
| Record control number |
(OCoLC)860394433 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
|---|
| Uniform title |
Methods in enzymology ; |
| Volume number/sequential designation |
v. 533. |
| International Standard Serial Number |
0076-6879 |
| 9 (RLIN) |
97896 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/00766879/533">https://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/00766879/533</a> |
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YBP Library Services |
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YANK |
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11262028 |
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92 |
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ATIST |