Branched Actin networks in dendritic cell biology (Record no. 373533)

000 -LEADER
fixed length control field 03030nam a22002897a 4500
003 - CONTROL NUMBER IDENTIFIER
control field AT-ISTA
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20190813100031.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 180627b xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency IST
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Leithner, Alexander
9 (RLIN) 4261
245 ## - TITLE STATEMENT
Title Branched Actin networks in dendritic cell biology
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc. IST Austria
Date of publication, distribution, etc. 2018
500 ## - GENERAL NOTE
General note Thesis
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Formatted contents note Abstract
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Formatted contents note Acknowledgments
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Formatted contents note List of Figures
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Formatted contents note List of Symbols/Abbreviations
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Formatted contents note 1 Introduction
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Formatted contents note 2 The role of leading edge protrusions in leukocyte cell migration
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Formatted contents note 3 Immoralized hematopoietic precursor cells - a genetically tractable tool for the study of dendritic cell biology
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Formatted contents note 4 The dendritic cell branched F-actin cytoskeleton in immunological synapse formation
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Formatted contents note 5 Concluding remarks
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Formatted contents note References
520 ## - SUMMARY, ETC.
Summary, etc. In the here presented thesis, we explore the role of branched actin networks in cell migration and antigen presentation, the two most relevant processes in dendritic cell biology. <br/> Branched actin networks construct lamellipodial protrusions at the leading edge of migrating cells. These are typically seen as adhesive structures, which mediate force transduction to the extracellular matrix that leads to forward locomotion. We ablated Arp2/3 nucleation promoting factor WAVE in DCs and found that the resulting cells lack lamellipodial protrusions. Instead, depending on the maturation state, one or multiple filopodia were formed. By challenging these cells in a variety of migration assays we found that lamellipodial protrusions are dispensable for the locomotion of leukocytes and actually dampen the speed of migration. However, lamellipodia are critically required to negotiate complex environments that DCs experience while they travel to the next draining lymph node. Taken together our results suggest that leukocyte lamellipodia have rather a sensory- than a force transducing function.<br/> Furthermore, we show for the first time structure and dynamics of dendritic cell F-actin at the immunological synapse with naïve T cells. Dendritic cell F-actin appears as dynamic foci that are nucleated by the Arp2/3 complex. WAVE ablated dendritic cells show increased membrane tension, leading to an altered ultrastructure of the immunological synapse and severe T cell priming defects. These results point towards a previously unappreciated role of the cellular mechanics of dendritic cells in T cell activation.<br/> Additionally, we present a novel cell culture based system for the differentiation of dendritic cells from conditionally immortalized hematopoietic precursors. These precursor cells are genetically tractable via the CRISPR/Cas9 system while they retain their ability to differentiate into highly migratory dendritic cells and other immune cells. This will foster the study of all aspects of dendritic cell biology and beyond.
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://doi.org/10.15479/AT:ISTA:th_998">https://doi.org/10.15479/AT:ISTA:th_998</a>
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Permanent Location Current Location Date acquired Barcode Date last seen Price effective from Koha item type
  Not Lost       Library Library 2018-06-27 AT-ISTA#001523 2018-11-06 2018-06-27 Book

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