Design and characterization of methods and biological components to realize synthetic neurotransmission (Record no. 374035)

000 -LEADER
fixed length control field 03109ntm a22003137a 4500
003 - CONTROL NUMBER IDENTIFIER
control field AT-ISTA
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20190904125309.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 190829s2018 au ||||| m||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency IST
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name McKenzie, Catherine
9 (RLIN) 5933
245 ## - TITLE STATEMENT
Title Design and characterization of methods and biological components to realize synthetic neurotransmission
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc. IST Austria
Date of publication, distribution, etc. 2018
500 ## - GENERAL NOTE
General note Thesis
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Formatted contents note Abstract
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Formatted contents note Acknowledgements and Dedication
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Formatted contents note Table of Contents
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Formatted contents note List of Figures
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Formatted contents note List of Tables
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Formatted contents note List of Abbreviations
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Formatted contents note Introduction
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Formatted contents note 1 Synthetic Neurotransmission: Manipulating Neuronal Communication with Intrinsic Encoding and Connectivity
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Formatted contents note 2 Repurposing a Methionine-gated Ionotropic Receptor for Orthogonal Control of Bioelectrical Activity in Mammalian Cells
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Formatted contents note 3 Isolation of Synaptic Vesicles from Genetically Engineered Cultured Neurons
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Formatted contents note 4 Towards Synthetic Neurotransmission
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Formatted contents note References
520 ## - SUMMARY, ETC.
Summary, etc. A major challenge in neuroscience research is to dissect the circuits that orchestrate behavior in health and disease. Proteins from a wide range of non-mammalian species, such as microbial opsins, have been successfully transplanted to specific neuronal targets to override their natural communication patterns. The goal of our work is to manipulate synaptic communication in a manner that closely incorporates the functional intricacies of synapses by preserving temporal encoding (i.e. the firing pattern of the presynaptic neuron) and connectivity (i.e. target specific synapses rather than specific neurons). Our strategy to achieve this goal builds on the use of non-mammalian transplants to create a synthetic synapse. The mode of modulation comes from pre-synaptic uptake of a synthetic neurotransmitter (SN) into synaptic vesicles by means of a genetically targeted transporter selective for the SN. Upon natural vesicular release, exposure of the SN to the synaptic cleft will modify the post-synaptic potential through an orthogonal ligand gated ion channel. To achieve this goal we have functionally characterized a mixed cationic methionine-gated ion channel from Arabidopsis thaliana, designed a method to functionally characterize a synthetic transporter in isolated synaptic vesicles without the need for transgenic animals, identified and extracted multiple prokaryotic uptake systems that are substrate specific for methionine (Met), and established a primary/cell line co-culture system that would allow future combinatorial testing of this orthogonal transmitter-transporter-channel trifecta. Synthetic synapses will provide a unique opportunity to manipulate synaptic communication while maintaining the electrophysiological integrity of the pre-synaptic cell. In this way, information may be preserved that was generated in upstream circuits and that could be essential for concerted function and information processing.
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://doi.org/10.15479/at:ista:th_1055">https://doi.org/10.15479/at:ista:th_1055</a>
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme
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Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Permanent Location Current Location Date acquired Barcode Date last seen Price effective from Koha item type
  Not Lost     1 Library Library 2019-08-29 AT-ISTA#001891 2019-08-29 2019-08-29 Book

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