Gene expression technology [electronic resource] / edited by David V. Goeddel.

Contributor(s): Goeddel, David V
Material type: TextTextSeries: Methods in enzymology: v. 185.Publisher: San Diego : Academic Press, �1990Description: 1 online resource (xxxi, 681 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 0121820866; 9780121820862Subject(s): Gene expression -- Technique | Molecular cloning | Cloning, Molecular | Gene Expression | Genetic Techniques | Expressivit�e g�en�etique -- Technologie | Gene expression -- Technique | Molecular cloning | Genexpressie | Wetenschappelijke technieken | Laboratoriumonderzoek | Genexpression | Mikroorganismus | Aufsatzsammlung | S�augetiere | cDNS | Genklonierung | Technologie | Genetic engineeringGenre/Form: Electronic books.Additional physical formats: Print version:: No titleDDC classification: 574.87322 LOC classification: QP601 | .M49 v.185Online resources: Click here to access online
Contents:
Systems for heterologous gene expression / David V. Goeddel and David V. Goeddel -- Expression of heterologous proteins in escherichia coli / Larry Gold and David V. Goeddel -- Design and construction of expression plasmid vectors in escherichia coli / Paulina Balbas, Francisco Bolivar and David V. Goeddel -- Promoter recognition and mRNA initiation by Escherichia coli E[sigma]70 / Jay D. Gralla and David V. Goeddel -- Use of Escherichia coli trp promoter for direct expression of proteins / Daniel G. Yansura, Dennis J. Henner and David V. Goeddel -- Use of T7 RNA polymerase to direct expression of cloned genes / F. William Studier [and others] -- High-level translation initiation / Larry Gold, Gary D. Stormo and David V. Goeddel -- Enhanced translational efficiency with two-cistron expression system / Brigitte E. Schoner [and others] -- Sequences within ribosome binding site affecting messenger RNA translatability and method to direct ribosomes to single messenger RNA species / Herman A. De Boer, Anna S. Hui and David V. Goeddel -- Vector for enhanced translation of foreign genes in Escherichia coli / Peter O. Olins, Shaukat H. Rangwala and David V. Goeddel -- Minimizing proteolysis in Escherichia coli: genetic solutions / Susan Gottesman and David V. Goeddel -- Gene fusions for purpose of expression: An introduction / Mathias Uhl�en, Tomas Moks and David V. Goeddel -- Fusions to staphylococcal protein A / Bj�orn Nilsson, Lars Abrahms�en and David V. Goeddel -- Expression as trpE fusion / Daniel G. Yansura and David V. Goeddel -- Engineering Escherchia coli to secrete heterologous gene products / Joan A. Stader, Thomas J. Silhavy and David V. Goeddel -- Refolding of recombinant proteins / Tadahiko Kohno [and others] -- Expression of heterologous genes in Bacillus subtilis / Dennis J. Henner and David V. Goeddel -- Regulated promoter for high-level expression of heterologous genes in Bacillus subtilis / Stuart F.J. Le Grice and David V. Goeddel -- System for secretion of heterologous proteins in Bacillus subtilis / Vasantha Nagarajan and David V. Goeddel -- Inducible expression of regulatory genes in Bacillus subtilis / Dennis J. Henner and David V. Goeddel.
Heterologous gene expression in yeast / Scott D. Emr and David V. Goeddel -- Propagation and expression of cloned genes in yeast: 2-[mu]m circle-based vectors / Alan B. Rose, James R. Broach and David V. Goeddel -- Manipulating yeast genome using plasmid vectors / Tim Stearns [and others] -- Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast / Lawrence M. Mylin [and others] -- Expression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter / Virginia L. Price [and others] -- Induced expression using yeast copper metallothionein promoter / Tina Etcheverry and David V. Goeddel -- High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene / S.M. Kingsman [and others] -- Glyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteins / Steven Rosenberg [and others] -- Superimposition of temperature regulation on yeast promoters / Andrzej Z. Sledziewski [and others] -- Sequence and structural requirements for efficient translation in yeast / Thomas F. Donahue, Mark A. Cigan and David V. Goeddel -- Vacuolar proteases in yeast Saccharomyces cerevisiae / Elizabeth W. Jones and David V. Goeddel -- Detection and inhibition of ubiquitin-dependent proteolysis / Keith D. Wilkinson and David V. Goeddel -- Contranslational amino-terminal processing / Richard L. Kendall [and others] -- [alpha]-Factor leader-directed secretion of heterologous proteins from yeast / Anthony J. Brake and David V. Goeddel -- Use of heterologous and homologous signal sequences for secretion of heterologous proteins from yeast / Ronald A. Hitzeman [and others] -- Isolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects / Clinton E. Ballou and David V. Goeddel -- Molecular and genetic approach to enhancing protein secretion / Vanessa Chisholm [and others].
Expression of heterologous genes in mammalian cells / Arthur D. Levinson and David V. Goeddel -- Vectors used for expression in mammalian cells / Randal J. Kaufman and David V. Goeddel -- Assembly of enhancers, promoters, and splice signals to control expression of transferred genes / Michael Kriegler and David V. Goeddel -- Methods for introducing DNA into mammalian cells / Wayne A. Keown [and others] -- Selection and coamplification of heterologous genes in mammalian cells / Randal J. Kaufman and David V. Goeddel -- Optimizing cell and culture environment for production of recombinant proteins / Jennie P. Mather and David V. Goeddel -- Analysis of synthesis, processing, and secretion of proteins expressed in mammalian cells / Andrew J. Dorner, Kaufman Randal J. and David V. Goeddel -- Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil / Bruce H. Horwitz, Daniel Dimaio and David V. Goeddel.
Summary: The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.
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Includes indexes.

Includes bibliographical references.

Systems for heterologous gene expression / David V. Goeddel and David V. Goeddel -- Expression of heterologous proteins in escherichia coli / Larry Gold and David V. Goeddel -- Design and construction of expression plasmid vectors in escherichia coli / Paulina Balbas, Francisco Bolivar and David V. Goeddel -- Promoter recognition and mRNA initiation by Escherichia coli E[sigma]70 / Jay D. Gralla and David V. Goeddel -- Use of Escherichia coli trp promoter for direct expression of proteins / Daniel G. Yansura, Dennis J. Henner and David V. Goeddel -- Use of T7 RNA polymerase to direct expression of cloned genes / F. William Studier [and others] -- High-level translation initiation / Larry Gold, Gary D. Stormo and David V. Goeddel -- Enhanced translational efficiency with two-cistron expression system / Brigitte E. Schoner [and others] -- Sequences within ribosome binding site affecting messenger RNA translatability and method to direct ribosomes to single messenger RNA species / Herman A. De Boer, Anna S. Hui and David V. Goeddel -- Vector for enhanced translation of foreign genes in Escherichia coli / Peter O. Olins, Shaukat H. Rangwala and David V. Goeddel -- Minimizing proteolysis in Escherichia coli: genetic solutions / Susan Gottesman and David V. Goeddel -- Gene fusions for purpose of expression: An introduction / Mathias Uhl�en, Tomas Moks and David V. Goeddel -- Fusions to staphylococcal protein A / Bj�orn Nilsson, Lars Abrahms�en and David V. Goeddel -- Expression as trpE fusion / Daniel G. Yansura and David V. Goeddel -- Engineering Escherchia coli to secrete heterologous gene products / Joan A. Stader, Thomas J. Silhavy and David V. Goeddel -- Refolding of recombinant proteins / Tadahiko Kohno [and others] -- Expression of heterologous genes in Bacillus subtilis / Dennis J. Henner and David V. Goeddel -- Regulated promoter for high-level expression of heterologous genes in Bacillus subtilis / Stuart F.J. Le Grice and David V. Goeddel -- System for secretion of heterologous proteins in Bacillus subtilis / Vasantha Nagarajan and David V. Goeddel -- Inducible expression of regulatory genes in Bacillus subtilis / Dennis J. Henner and David V. Goeddel.

Heterologous gene expression in yeast / Scott D. Emr and David V. Goeddel -- Propagation and expression of cloned genes in yeast: 2-[mu]m circle-based vectors / Alan B. Rose, James R. Broach and David V. Goeddel -- Manipulating yeast genome using plasmid vectors / Tim Stearns [and others] -- Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast / Lawrence M. Mylin [and others] -- Expression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter / Virginia L. Price [and others] -- Induced expression using yeast copper metallothionein promoter / Tina Etcheverry and David V. Goeddel -- High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene / S.M. Kingsman [and others] -- Glyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteins / Steven Rosenberg [and others] -- Superimposition of temperature regulation on yeast promoters / Andrzej Z. Sledziewski [and others] -- Sequence and structural requirements for efficient translation in yeast / Thomas F. Donahue, Mark A. Cigan and David V. Goeddel -- Vacuolar proteases in yeast Saccharomyces cerevisiae / Elizabeth W. Jones and David V. Goeddel -- Detection and inhibition of ubiquitin-dependent proteolysis / Keith D. Wilkinson and David V. Goeddel -- Contranslational amino-terminal processing / Richard L. Kendall [and others] -- [alpha]-Factor leader-directed secretion of heterologous proteins from yeast / Anthony J. Brake and David V. Goeddel -- Use of heterologous and homologous signal sequences for secretion of heterologous proteins from yeast / Ronald A. Hitzeman [and others] -- Isolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects / Clinton E. Ballou and David V. Goeddel -- Molecular and genetic approach to enhancing protein secretion / Vanessa Chisholm [and others].

Expression of heterologous genes in mammalian cells / Arthur D. Levinson and David V. Goeddel -- Vectors used for expression in mammalian cells / Randal J. Kaufman and David V. Goeddel -- Assembly of enhancers, promoters, and splice signals to control expression of transferred genes / Michael Kriegler and David V. Goeddel -- Methods for introducing DNA into mammalian cells / Wayne A. Keown [and others] -- Selection and coamplification of heterologous genes in mammalian cells / Randal J. Kaufman and David V. Goeddel -- Optimizing cell and culture environment for production of recombinant proteins / Jennie P. Mather and David V. Goeddel -- Analysis of synthesis, processing, and secretion of proteins expressed in mammalian cells / Andrew J. Dorner, Kaufman Randal J. and David V. Goeddel -- Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil / Bruce H. Horwitz, Daniel Dimaio and David V. Goeddel.

The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.

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