Guide to molecular cloning techniques / edited by Shelby L. Berger, Alan R. Kimmel.

Contributor(s): Berger, Shelby L [editor.] | Kimmel, Alan R [editor.]Material type: TextTextSeries: Methods in enzymology ; v. 152.Publication details: Orlando : Academic Press, 1987. Description: 1 online resource (xl, 812 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceSubject(s): Molecular cloning | Molecular cloning -- Technique | Cloning -- Technique | DNA | Recombinant DNA | Cloning, Molecular -- methods | DNA, Recombinant | Cloning, Molecular | DNA | Clonage moléculaire -- Technique | Clonage -- Technique | ADN | Clonage moléculaire | ADN recombinant | 42.15 cytology, cell biology and cell physiology | Cloning | Clonage | ADN = Acide désoxyribonucléique | Génétique | Manuels | Molecular cloning | Genklonierung | Aufsatzsammlung | Klonierung | Nucleinsäuren | Methode | Kloneren | Biologia molecular | Laboratorio | Acido nucleico | DNA recombinante | Bacteria | Clonagem | RNA | Molecular biology | Laboratory | Nucleic acids | Recombinant DNA | Cloning | ClonesGenre/Form: Electronic books. | Aufsatzsammlung. | Textbooks (form) Additional physical formats: Print version:: Guide to molecular cloning techniques.DDC classification: 612.0151 LOC classification: QH442QP601 | .C8 v.152 1987NLM classification: W1 | QH 442.2Other classification: 42.15 | 42.13 | 58.30 | BIO 180f | BIO 220f | CHE 860f | WC 4355 | WD 5050 | A9e | D6c1 Online resources: Click here to access online
Contents:
Section I. Requirements for a molecular biology laboratory -- Section II. General methods for isolating and characterizing nucleic acids -- Section III. Enzymatic techniques and recombinant DNA technology -- Section IV. Restriction enzymes -- Section V. Growth and maintenance of bacteria -- Section VI. Genomic cloning -- Section VII. Preparation and characterization of RNA -- Section VIII. Preparation of cDNA and the generation of cDNA libraries -- Section IX. Selection of clones from libraries -- Section X. Identification and characterization of specific clones.
Action note: digitized 2012 committed to preserveSummary: Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA and the generation of cDNA libraries. Selection of clones from libraries. Identification and characterization of specific clones.
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Includes bibliographical references and index.

880-01 Section I. Requirements for a molecular biology laboratory -- Section II. General methods for isolating and characterizing nucleic acids -- Section III. Enzymatic techniques and recombinant DNA technology -- Section IV. Restriction enzymes -- Section V. Growth and maintenance of bacteria -- Section VI. Genomic cloning -- Section VII. Preparation and characterization of RNA -- Section VIII. Preparation of cDNA and the generation of cDNA libraries -- Section IX. Selection of clones from libraries -- Section X. Identification and characterization of specific clones.

Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA and the generation of cDNA libraries. Selection of clones from libraries. Identification and characterization of specific clones.

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