Guide to molecular cloning techniques [electronic resource] / edited by Shelby L. Berger, Alan R. Kimmel.

Contributor(s): Berger, Shelby L | Kimmel, Alan R
Material type: TextTextSeries: Methods in enzymology: v.152.Publisher: Orlando : Academic Press, 1987Description: 1 online resource (xl, 812 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceSubject(s): Molecular cloning | Cloning, Molecular -- methods | DNA, Recombinant | Clonage mol�eculaire -- Technique | Clonage -- Technique | ADN | Molecular cloning | Kloneren | Genklonierung | Aufsatzsammlung | Klonierung | Nucleins�auren | Methode | Biologia molecular | Laboratorio | Acido nucleico | DNA recombinante | Bacteria | Clonagem | RNA | Molecular biology | Laboratory | Nucleic acids | Recombinant DNA | Cloning | ClonesGenre/Form: Electronic books.Additional physical formats: Print version:: No titleDDC classification: 612.0151 LOC classification: QH442QP601 | .C8 v.152 1987Other classification: 42.15 Online resources: Click here to access online Summary: Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA and the generation of cDNA libraries. Selection of clones from libraries. Identification and characterization of specific clones.
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Includes bibliographical references and index.

Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA and the generation of cDNA libraries. Selection of clones from libraries. Identification and characterization of specific clones.

Description based on print version record.

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