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Laboratory methods in enzymology. Volume 530, RNA / edited by Jon Lorsch, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Contributor(s): Lorsch, Jon [editor.]Material type: TextTextSeries: Methods in enzymology ; v. 530.Publisher: Amsterdam ; Boston : Elsevier/Academic Press, 2013Edition: First editionDescription: 1 online resource (lviii, 413 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9780124200517; 0124200516Subject(s): Enzymes | Biochemistry -- Technique | Biotechnology -- Methodology | Molecular biology -- Methodology | SCIENCE -- Life Sciences -- Biochemistry | Biochemistry -- Technique | Biotechnology -- Methodology | Enzymes | Molecular biology -- Methodology | Sequence Analysis, RNA -- methods | Biotechnology -- methods | Biotechnology -- istrumentation | Molecular Biology -- methods | Molecular Biology -- istrumentationGenre/Form: Electronic books. | Electronic books. Additional physical formats: Print version:: Laboratory methods in enzymology.DDC classification: 572/.7 LOC classification: QP601 | .L33 2013ebOnline resources: Click here to access online
Contents:
Computational prediction of RNA secondary structure / Walter N. Moss -- Reverse-transcription PCR (RT-PCR) / Julia Bachman -- Northern blotting / Shan L. He and Rachel Green -- Explanatory chapter: nuclease protection assays / Elizabeth Eyler -- In vitro transcription from plasmid or PCR-amplified DNA / Julie L. Brunelle and Rachel Green -- Measurement of in vivo RNA synthesis rates / David S. McPheeters and Jo Ann Wise -- Method for measuring mRNA decay rate in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Analysis of polysomes from bacteria / Daoming Qin and Kurt Fredrick -- Polysome profile analysis : yeast / Martin Pospísek and Leos Valásek -- Polysome analysis of mammalian cells / Shan L. He and Rachel Green -- Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates / Shimyn Slomovic and Gadi Schuster -- Circularized RT-PCR (cRT-PCR) : analysis of the 5' ends, 3' ends, and poly(A) tails of RNA / Shimyn Slomovic and Gadi Schuster -- RNA radiolabeling / Rishi Porecha and Daniel Herschlag -- Fluorescently labeling synthetic RNAs / Max Greenfield and Daniel Herschlag -- Analysis of RNA by analytical polyacrylamide gel electrophoresis / Alexey Petrov, Albet Tsa, and Joseph D. Puglisi -- RNA purification by preparative polyacrylamide gel electrophoresis / Alexey Petrov, Tinghe Wu, Elisabetta Viani Puglisi, and Joseph D. Puglisi -- Explanatory chapter : nucleic acid concentration determination / Sarah Kolitz and Jon Lorsch -- RNA purification : precipitation methods / Sarah E. Walker and Jon Lorsch -- Reverse transcriptase dideoxy sequencing of RNA / Sarah E. Walker and Jon Lorsch -- RNA structure experimental analysis : chemical modification / Zhili Xu and Gloria Culver -- Structural analysis of RNA backbone using in-line probing / Ali Nahvi and Rachel Green.
Summary: Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brou.
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Includes bibliographical references and indexes.

Computational prediction of RNA secondary structure / Walter N. Moss -- Reverse-transcription PCR (RT-PCR) / Julia Bachman -- Northern blotting / Shan L. He and Rachel Green -- Explanatory chapter: nuclease protection assays / Elizabeth Eyler -- In vitro transcription from plasmid or PCR-amplified DNA / Julie L. Brunelle and Rachel Green -- Measurement of in vivo RNA synthesis rates / David S. McPheeters and Jo Ann Wise -- Method for measuring mRNA decay rate in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Analysis of polysomes from bacteria / Daoming Qin and Kurt Fredrick -- Polysome profile analysis : yeast / Martin Pospísek and Leos Valásek -- Polysome analysis of mammalian cells / Shan L. He and Rachel Green -- Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates / Shimyn Slomovic and Gadi Schuster -- Circularized RT-PCR (cRT-PCR) : analysis of the 5' ends, 3' ends, and poly(A) tails of RNA / Shimyn Slomovic and Gadi Schuster -- RNA radiolabeling / Rishi Porecha and Daniel Herschlag -- Fluorescently labeling synthetic RNAs / Max Greenfield and Daniel Herschlag -- Analysis of RNA by analytical polyacrylamide gel electrophoresis / Alexey Petrov, Albet Tsa, and Joseph D. Puglisi -- RNA purification by preparative polyacrylamide gel electrophoresis / Alexey Petrov, Tinghe Wu, Elisabetta Viani Puglisi, and Joseph D. Puglisi -- Explanatory chapter : nucleic acid concentration determination / Sarah Kolitz and Jon Lorsch -- RNA purification : precipitation methods / Sarah E. Walker and Jon Lorsch -- Reverse transcriptase dideoxy sequencing of RNA / Sarah E. Walker and Jon Lorsch -- RNA structure experimental analysis : chemical modification / Zhili Xu and Gloria Culver -- Structural analysis of RNA backbone using in-line probing / Ali Nahvi and Rachel Green.

Print version record.

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brou.

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