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Endosome signalling. Part A / volume editor, P. Michael Conn.

Contributor(s): Conn, P. Michael [editor.]Material type: TextTextSeries: Methods in enzymology ; v. 534.Publisher: Amsterdam : Academic Press, 2014Copyright date: ©2014Edition: First editionDescription: 1 online resource (xix, 378 pages) : illustrations (chiefly color)Content type: text Media type: computer Carrier type: online resourceISBN: 9780123984821; 0123984823Other title: Endosome signaling. Part ASubject(s): Endosomes | Cell interaction | Cell Communication | Endosomes | Endosomes | Cellules -- Interaction | SCIENCE -- Life Sciences -- Anatomy & Physiology | Cell interaction | EndosomesGenre/Form: Electronic books. | Electronic books. | Ebook. | Electronic books -- Ebook. Additional physical formats: Print version:: Endosome signalling. Part A.DDC classification: 571.655 LOC classification: QP601 | .M37 2014ebNLM classification: W1 | QU 350Online resources: Click here to access online
Contents:
Assessment of insulin proteolysis in rat liver endosomes: its relationship to intracellular insulin signaling -- A biomolecular fluorescent complementation screen reveals complex roles of endosomes in Ras-mediated signaling -- TGF[beta] in endosomal signaling -- Annexins and endosomal signaling -- Analysis, regulation, and roles of endosomal phosphoinositides -- Mild fixation and permeabilization protocol for preseving structures of endosomes, focal adhesions, and actin flimaments during imunofluorescence analysis -- Characterizing and measuring endocytosis of lipid-binding effectors in mammalian cells -- Measuring the role for met endosomal signaling in tumorigenesis -- Intracellular toll-like receptor recruitment and cleavage in endosomal/lysosomal organelles -- Assessment of the toll-like receptor 3 pathway in endosomal signaling -- Labeling of platelet-derived growth factor by reversible biotinylation to visualize its endocytosis by microscopy -- Endosomal signaling and oncogenesis -- ROS-containing endosomal compartments: implications for signaling -- SARA and RNF11 at the crossroads of EGFR signalling and trafficking -- p18/LAMTOR1: a late endosome/lysosome-specific anchor protein for the mTORC1/MAPK signaling pathway -- Vascular endothelial growth factor A-stimulated signaling from endosomes in primary endothelial cells -- Assessment of internalization and endosomal signaling: studies with insulin and EGF -- Quantitative proteomic analysis of compartmentalized signaling networks -- Separation of magnetically isolated TNF receptosomes from mitochondria -- Deubiquitinases and their emerging roles in [beta]-arrestin-mediated signaling -- TSLP expression induced via toll-like receptor pathways in human keratinocytes -- Endosomal signaling by protease-activated receptors -- Investigating signalling consequences of GPCR trafficking in the endocytic pathway -- MICAL-like1 in endosomal signaling.
Summary: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of two volumes on endosome signaling and includes chapters on such topics as measurement of entry into the endosomal compartment by multi-parametric image analysis, assessment of peptide internalization and endosomal signaling, and VEGF-A in endosomal signaling. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers endosome signalingContains chapters on such topics as measurement of biological effects of endosomal proteolysis of internalized insulin and multi-vesicular endosome biogenesis.
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Includes bibliographical references and indexes.

Assessment of insulin proteolysis in rat liver endosomes: its relationship to intracellular insulin signaling -- A biomolecular fluorescent complementation screen reveals complex roles of endosomes in Ras-mediated signaling -- TGF[beta] in endosomal signaling -- Annexins and endosomal signaling -- Analysis, regulation, and roles of endosomal phosphoinositides -- Mild fixation and permeabilization protocol for preseving structures of endosomes, focal adhesions, and actin flimaments during imunofluorescence analysis -- Characterizing and measuring endocytosis of lipid-binding effectors in mammalian cells -- Measuring the role for met endosomal signaling in tumorigenesis -- Intracellular toll-like receptor recruitment and cleavage in endosomal/lysosomal organelles -- Assessment of the toll-like receptor 3 pathway in endosomal signaling -- Labeling of platelet-derived growth factor by reversible biotinylation to visualize its endocytosis by microscopy -- Endosomal signaling and oncogenesis -- ROS-containing endosomal compartments: implications for signaling -- SARA and RNF11 at the crossroads of EGFR signalling and trafficking -- p18/LAMTOR1: a late endosome/lysosome-specific anchor protein for the mTORC1/MAPK signaling pathway -- Vascular endothelial growth factor A-stimulated signaling from endosomes in primary endothelial cells -- Assessment of internalization and endosomal signaling: studies with insulin and EGF -- Quantitative proteomic analysis of compartmentalized signaling networks -- Separation of magnetically isolated TNF receptosomes from mitochondria -- Deubiquitinases and their emerging roles in [beta]-arrestin-mediated signaling -- TSLP expression induced via toll-like receptor pathways in human keratinocytes -- Endosomal signaling by protease-activated receptors -- Investigating signalling consequences of GPCR trafficking in the endocytic pathway -- MICAL-like1 in endosomal signaling.

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of two volumes on endosome signaling and includes chapters on such topics as measurement of entry into the endosomal compartment by multi-parametric image analysis, assessment of peptide internalization and endosomal signaling, and VEGF-A in endosomal signaling. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers endosome signalingContains chapters on such topics as measurement of biological effects of endosomal proteolysis of internalized insulin and multi-vesicular endosome biogenesis.

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