Normal view MARC view ISBD view

Biology of restriction-modification systems at the single-cell and population level

By: Pleska, Maros.
Material type: materialTypeLabelBookPublisher: IST Austria 2017
Contents:
Abstract Acknowledgments Preface About the author List of publications appearing in thesis Table of contents List of figures List of tables List of symbols/abbreviations 1 Introduction 2 Effects of mutations in phage restriction sites during escape from restriction-modification 3 Bacterial autoimmunity due to a restriction-modification system 4 Phage-host population dynamics promotes prophage acquisition in bacteria with innate immunity References
Summary: Restriction-modification (RM) represents the simplest and possibly the most widespread mechanism of self/non-self discrimination in nature. In order to provide bacteria with immunity against bacteriophages and other parasitic genetic elements, RM systems rely on a balance between two enzymes: the restriction enzyme, which cleaves non-self DNA at specific restriction sites, and the modification enzyme, which tags the host’s DNA as self and thus protects it from cleavage. In this thesis, I use population and single-cell level experiments in combination with mathematical modeling to study different aspects of the interplay between RM systems, bacteria and bacteriophages. First, I analyze how mutations in phage restriction sites affect the probability of phage escape – an inherently stochastic process, during which phages accidently get modified instead of restricted. Next, I use single-cell experiments to show that RM systems can, with a low probability, attack the genome of their bacterial host and that this primitive form of autoimmunity leads to a tradeoff between the evolutionary cost and benefit of RM systems. Finally, I investigate the nature of interactions between bacteria, RM systems and temperate bacteriophages to find that, as a consequence of phage escape and its impact on population dynamics, RM systems can promote acquisition of symbiotic bacteriophages, rather than limit it. The results presented here uncover new fundamental biological properties of RM systems and highlight their importance in the ecology and evolution of bacteria, bacteriophages and their interactions.
List(s) this item appears in: IST Austria Thesis 2018
Tags from this library: No tags from this library for this title. Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Call number Status Date due Barcode Item holds
Book Book Library
Available AT-ISTA#001534
Total holds: 0

Thesis

Abstract
Acknowledgments
Preface
About the author
List of publications appearing in thesis
Table of contents
List of figures
List of tables
List of symbols/abbreviations
1 Introduction
2 Effects of mutations in phage restriction sites during escape from restriction-modification
3 Bacterial autoimmunity due to a restriction-modification system
4 Phage-host population dynamics promotes prophage acquisition in bacteria with innate immunity
References

Restriction-modification (RM) represents the simplest and possibly the most widespread mechanism of self/non-self discrimination in nature. In order to provide bacteria with immunity against bacteriophages and other parasitic genetic elements, RM systems rely on a balance between two enzymes: the restriction enzyme, which cleaves non-self DNA at specific restriction sites, and the modification enzyme, which tags the host’s DNA as self and thus protects it from cleavage. In this thesis, I use population and single-cell level experiments in combination with mathematical modeling to study different aspects of the interplay between RM systems, bacteria and bacteriophages. First, I analyze how mutations in phage restriction sites affect the probability of phage escape – an inherently stochastic process, during which phages accidently get modified instead of restricted. Next, I use single-cell experiments to show that RM systems can, with a low probability, attack the genome of their bacterial host and that this primitive form of autoimmunity leads to a tradeoff between the evolutionary cost and benefit of RM systems. Finally, I investigate the nature of interactions between bacteria, RM systems and temperate bacteriophages to find that, as a consequence of phage escape and its impact on population dynamics, RM systems can promote acquisition of symbiotic bacteriophages, rather than limit it. The results presented here uncover new fundamental biological properties of RM systems and highlight their importance in the ecology and evolution of bacteria, bacteriophages and their interactions.

There are no comments for this item.

Log in to your account to post a comment.

Powered by Koha

//