Eicosanoid protocols / edited by Elias A. Lianos.

Contributor(s): Lianos, Elias A
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 120.Publisher: Totowa, N.J. : Humana Press, ©1999Description: 1 online resource (xi, 296 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592592630; 0585237689; 9780585237688; 1592592635; 9780896036673; 0896036677Subject(s): Eicosanoids -- Laboratory manuals | Cyclooxygenases -- Laboratory manuals | Eicosanoids -- genetics | Gene Expression Regulation, Enzymologic | Genetic Techniques | Immunoenzyme Techniques | Receptors, Eicosanoid | SCIENCE -- Life Sciences -- Biochemistry | Cyclooxygenases | Eicosanoids | eicosanoids | biologische technieken | biological techniques | protocollen | protocols | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Electronic books. | Handbooks and manuals. Additional physical formats: Print version:: Eicosanoid protocols.DDC classification: 572/.57 LOC classification: QP752.E53 | E35 1999ebOnline resources: Click here to access online
Contents:
Use of a Retroviral Vector for Expression of a Foreign Gene / Brent W. Miller and Aubrey R. Morrison -- Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues / William S. Powell -- Assessment of Cyclooxygenase RNA Expression by Northern Hybridization / Zhonghong Guan and Aubrey R. Morrison -- Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin / ShaAvhree Y. Buckman [and others] -- Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography / Mukut Sharma, Beatrice Hucke, and Elias A. Lianos -- Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry / M. Kerry O'Banion and John A. Olschowka -- Assessment of Cyclooxygenase Protein Expression by Western Blotting / Zhonghong Guan and Aubrey R. Morrison -- Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells / Alfred N. Fonteh -- Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction / Alfred N. Fonteh, William Reed, and James M. Samet -- Enzyme Immunoassays (EIA) of Eicosanoids / Kingsley Yin and Lloyd J. Forman -- Transcellular Regulation of Eicosanoid Biosynthesis / Karsten Gronert [and others] -- Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry / Samuel C. Blackman [and others] -- Epoxyeicosatrienoic Acids : Biosynthesis, Regulation, and Actions / John D. Imig -- Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts / Srinivasa T. Reddy and Harvey R. Herschman -- Assessment of 5-Lipoxygenase Activity and Cellular Distribution / Robert A. Lepley -- Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids / Eric G. Spokas, Joshua Rokach, and Patrick Y.-K. Wong -- Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition / Eric G. Spokas [and others] -- Novel Eicosanoids: Isoprostanes and Related Compounds / L. Jackson Roberts II [and others].
In: Springer ProtocolsSummary: In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids. Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.
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Includes bibliographical references and index.

Use of a Retroviral Vector for Expression of a Foreign Gene / Brent W. Miller and Aubrey R. Morrison -- Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues / William S. Powell -- Assessment of Cyclooxygenase RNA Expression by Northern Hybridization / Zhonghong Guan and Aubrey R. Morrison -- Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin / ShaAvhree Y. Buckman [and others] -- Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography / Mukut Sharma, Beatrice Hucke, and Elias A. Lianos -- Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry / M. Kerry O'Banion and John A. Olschowka -- Assessment of Cyclooxygenase Protein Expression by Western Blotting / Zhonghong Guan and Aubrey R. Morrison -- Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells / Alfred N. Fonteh -- Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction / Alfred N. Fonteh, William Reed, and James M. Samet -- Enzyme Immunoassays (EIA) of Eicosanoids / Kingsley Yin and Lloyd J. Forman -- Transcellular Regulation of Eicosanoid Biosynthesis / Karsten Gronert [and others] -- Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry / Samuel C. Blackman [and others] -- Epoxyeicosatrienoic Acids : Biosynthesis, Regulation, and Actions / John D. Imig -- Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts / Srinivasa T. Reddy and Harvey R. Herschman -- Assessment of 5-Lipoxygenase Activity and Cellular Distribution / Robert A. Lepley -- Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids / Eric G. Spokas, Joshua Rokach, and Patrick Y.-K. Wong -- Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition / Eric G. Spokas [and others] -- Novel Eicosanoids: Isoprostanes and Related Compounds / L. Jackson Roberts II [and others].

Print version record.

In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids. Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.

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