Hepatitis C protocols / edited by Johnson Yiu-Nam Lau ; forewords by T. Jake Liang [and others].

Contributor(s): Lau, Johnson Y. N. (Johnson Yiu-Nam)
Material type: TextTextSeries: Methods in molecular medicine: 19.Publisher: Totowa, N.J. : Humana Press, ©1998Description: 1 online resource (xxi, 621 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592592609; 1592592600; 0585235651; 9780585235653Subject(s): Hepatitis C virus -- Research -- Methodology | Diagnosis | Hepatitis C Antibodies | Hepatitis C Antigens | Genetics | Hepatitis C | Hepatitis, Viral, Human | Hepatitis Antibodies | Biology | Flaviviridae Infections | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Hepatitis Antigens | Biological Science Disciplines | Antibodies, Viral | Hepatitis | Virus Diseases | RNA Virus Infections | Antigens, Viral | Antigens | Disease | Antibodies | Liver Diseases | Natural Science Disciplines | Immunoglobulins | Digestive System Diseases | Biological Factors | Disciplines and Occupations | Serum Globulins | Immunoproteins | Chemicals and Drugs | Blood Proteins | Globulins | Proteins | Amino Acids, Peptides, and Proteins | MEDICAL -- Hepatology | Hépatite virale non-A, non-B -- Génétique | Antigène VHC | Anticorps anti-VHC | Hépatite virale non-A, non-B -- Diagnostic | Hepatitis-C | Wetenschappelijke technieken | Hépatite CGenre/Form: Electronic books. Additional physical formats: Print version:: Hepatitis C protocols.DDC classification: 616.3/623 LOC classification: QR201.H46 | H447 1998Other classification: 44.75 Online resources: Click here to access online
Contents:
Detection of anti-GOR antibodies in HCV infection / M.P. Manns and G. Michel -- Confirmation of HCV antibodies by the line immunoassay INNO-LIA HCV Ab III / G. Maertens [and others] -- Detection of HCV RNA in serum by reverse transcriptase-PCR and radiolabeled liquid hybridization / R.L. Hodinka -- Detection of HCV RNA in serum by reverse transcription polymerase chain reaction (RT-PCR) / K. Qian -- The AMPLICOR® HCV tests for the detection and quantitation of serum or plasma HCV RNA / K.A. Gutekunst [and others] -- Quantification of HCV RNA in clinical specimens by branched DNA (bDNA) technology / J.C. Wilber and M.S. Urdea -- Quality control of the polymerase chain reaction / M. Rosenstraus [and others] -- Preparation of genotype-specific HCV RNA transcripts for assessing HCV detection and quantification assays / J.J. Detmer [and others] -- Detection of HCV RNA in formalin-fixed, paraffin-embedded liver tissue by RT-PCR / J.Y.N. Lau -- Quantification of HCV RNA in liver tissue by bDNA assay / P.J. Dailey [and others] -- Hepatitis C virus : types, subtypes, and beyond / D.B. Smith and P. Simmonds -- Molecular evolutionary analysis : its application in the study of hepatitis C virus / M. Mizokami and J.Y. Lau -- Genotyping by type-specific primers that can type HCV types 1-6 / T. Ohno and M. Mizokami -- Genotyping hepatitis C virus by type-specific primers for PCR based on NS5 region / K. Chayama -- Determination of HCV genotypes by RFLP / F. Davidson and P. Simmonds -- HCV genotyping by the line probe assay INNO-LiPA HCV II / G. Maertens and L. Stuyver --Serological genotyping using synthetic peptides derived from the NS4 region / L.E. Prescott and P. Simmonds -- Determination of HCV quasispecies by cloning and sequencing / M. Mizokami and T. Ohno -- Detection of hepatitis C virus quasispecies heterogeneity by single-strand conformational polymorphism / R.P. González-Peralta -- Hepatitis C virus heteroduplex tracking assay : application to genotype determination, quasispecies analysis, and molecular evolution studies / A.J. Weiner [and others] -- In situ detection of HCV : an overview / F. Negro and J.Y.N. Lau -- In situ detection of hepatitis C viral antigens / R.P. González-Peralta and K. Krawczynski -- In situ hybridization and the detection and localization of HCV RNA / F. Negro, D. Pacchioni and G. Bussolati -- The in situ detection of PCR-amplified hepatitis C RNA / G.J. Nuovo and M.L. Alfieri -- Immunoelectron microscopic characterization of HCV / S. Watanabe, M. Kaito and M. Kohara -- Cloning and assembly of complex libraries of full-length HCV cDNA clones / A.A. Kolykhalov, K.E. Reed and C.M. Rice -- Use of the vaccinia virus/T7 expression system for studying HCV protein processing / E.V. Agapov, K.E. Reed and C.M. Rice -- Use of a discistronic vector for the quantitation of HCV IRES activity / J.A. Feller and J.Y.N. Lau -- Expression and dimerization of hepatitis C virus core protein in E. coli / S.Y. Lo and J.H. Ou -- Expression and characterization of the HCV NS2 protease / K.E. Reed and C.M. Rice -- Expression and characterization of HCV NS3 protease / E. D'Souza [and others] -- Expression and characterization of the HCV NS3 helicase domain / F. Preugschat [and others] -- Hepatitis C virus RNA-dependent RNA polymerase (NS5B polymerase) / C.H. Hagedorn -- Detection and molecular cloning of the extreme 3'-end of HCV / T. Tanaka and and K. Shimotohno -- In vitro stability of hepatitis C virus RNA / J.W.S. Fang -- Methods for the study of sequence-specific binding of proteins to the HCV RNA genome / L.M. Kaplan and R.T. Chung -- Role of immune response in HCV / M. Peters -- Determination of hepatitis C virus-specific CD4+ T-cell activity in PBMC / J.T. Gerlach, H. Diepolder and G.R. Pape -- Measurement of HCV-specific CD8+ cytotoxic T-cell activities in the peripheral blood by europium release assay / M. Imawari -- Determination of HCV-specific bulk CD8+ activity in liver / D.R. Nelson -- Characterization of HCV-specific cytotoxic T-lymphocytes from liver tissue / M.J. Koziel -- Production of human monoclonal antibodies to hepatitis C virus and their characterization / M.U. Mondelli and A. Cerino -- Specific detection of negative strand RNA of hepatitis C virus using chemical RNA modification / T. Gunji and K. Shimotohno -- Strand-specific rTth RT-PCR for the analysis of HCV replication / R.E. Lanford and D. Chavez -- Cell culture systems for the detection of HCV infection / Y.K. Shimizu and H. Yoshikura -- Replication and detection of hepatitis C virus in liver-derived cell lines / R.P. González-Peralta -- Primary human hepatocyte culture for the study of HCV / J.F. O'Connell [and others] -- A cultivation method for highly differentiated primary chimpanzee hepatocytes permissive for hepatitis C virus replication / R.E. Lanford and L. Estlack -- Construction of recombinant vaccinia virus expressing HCV genes / J.A. Feller -- Construction of recombinant adeno-associated virus (AAV) / M. Reiser and S. Zolotukhin -- Production of replication-deficient adenovirus recombinants / G.W.G. Wilkinson, C. Rickards and B.E. Clarke -- Generation of recombinant herpes simplex virus ampiicons / Z. Hong and A.D. Kwong -- Construction of recombinant sindbis-based expression vectors for the study of HCV genes and their products / B.D. Lindenbach, I. Frolov and C.M. Rice -- The chimpanzee model : contributions and considerations / E. Muchmore -- Liver transplantation as a model to study hepatitis C virus infection / H.M. Hassoba and T.L. Wright.
Action note: digitized 2010 committed to preserveSummary: In Hepatitis C Protocols, Johnson YN Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus infection and its disease, hepatitis C, which afflict an estimated 170 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV in liver tissue, to HCV genotyping and the detection and quantification of HCV in serum. In addition to these key protocols, the authoritative contributors also evaluate recombinant proteins in immunological test systems, including the identification of important antigenic epitopes, antibodies, and recombinant antigens. Molecular and immunological techniques to study this virus and its infection, as well as animal models and recombinant virus technology commonly used to express hepatitis C viral proteins, are also developed. Hepatitis C Protocols summarizes the state-of-the-art in today's research and understanding of the virology of hepatitis C virus. The methods and systems detailed in this book will play a key role in the development of more effective antivirals, in the development of vaccines, and in a deeper understanding of viral replication strategies, viron structure, and the mechanisms of viron assembly and release. A valuable new standard resource for all researchers and clinicians, Hepatitis C Protocols leads the way toward improved prevention, diagnosis, and treatment of viral hepatitis C.
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Includes bibliographical references and index.

Detection of anti-GOR antibodies in HCV infection / M.P. Manns and G. Michel -- Confirmation of HCV antibodies by the line immunoassay INNO-LIA HCV Ab III / G. Maertens [and others] -- Detection of HCV RNA in serum by reverse transcriptase-PCR and radiolabeled liquid hybridization / R.L. Hodinka -- Detection of HCV RNA in serum by reverse transcription polymerase chain reaction (RT-PCR) / K. Qian -- The AMPLICOR® HCV tests for the detection and quantitation of serum or plasma HCV RNA / K.A. Gutekunst [and others] -- Quantification of HCV RNA in clinical specimens by branched DNA (bDNA) technology / J.C. Wilber and M.S. Urdea -- Quality control of the polymerase chain reaction / M. Rosenstraus [and others] -- Preparation of genotype-specific HCV RNA transcripts for assessing HCV detection and quantification assays / J.J. Detmer [and others] -- Detection of HCV RNA in formalin-fixed, paraffin-embedded liver tissue by RT-PCR / J.Y.N. Lau -- Quantification of HCV RNA in liver tissue by bDNA assay / P.J. Dailey [and others] -- Hepatitis C virus : types, subtypes, and beyond / D.B. Smith and P. Simmonds -- Molecular evolutionary analysis : its application in the study of hepatitis C virus / M. Mizokami and J.Y. Lau -- Genotyping by type-specific primers that can type HCV types 1-6 / T. Ohno and M. Mizokami -- Genotyping hepatitis C virus by type-specific primers for PCR based on NS5 region / K. Chayama -- Determination of HCV genotypes by RFLP / F. Davidson and P. Simmonds -- HCV genotyping by the line probe assay INNO-LiPA HCV II / G. Maertens and L. Stuyver --Serological genotyping using synthetic peptides derived from the NS4 region / L.E. Prescott and P. Simmonds -- Determination of HCV quasispecies by cloning and sequencing / M. Mizokami and T. Ohno -- Detection of hepatitis C virus quasispecies heterogeneity by single-strand conformational polymorphism / R.P. González-Peralta -- Hepatitis C virus heteroduplex tracking assay : application to genotype determination, quasispecies analysis, and molecular evolution studies / A.J. Weiner [and others] -- In situ detection of HCV : an overview / F. Negro and J.Y.N. Lau -- In situ detection of hepatitis C viral antigens / R.P. González-Peralta and K. Krawczynski -- In situ hybridization and the detection and localization of HCV RNA / F. Negro, D. Pacchioni and G. Bussolati -- The in situ detection of PCR-amplified hepatitis C RNA / G.J. Nuovo and M.L. Alfieri -- Immunoelectron microscopic characterization of HCV / S. Watanabe, M. Kaito and M. Kohara -- Cloning and assembly of complex libraries of full-length HCV cDNA clones / A.A. Kolykhalov, K.E. Reed and C.M. Rice -- Use of the vaccinia virus/T7 expression system for studying HCV protein processing / E.V. Agapov, K.E. Reed and C.M. Rice -- Use of a discistronic vector for the quantitation of HCV IRES activity / J.A. Feller and J.Y.N. Lau -- Expression and dimerization of hepatitis C virus core protein in E. coli / S.Y. Lo and J.H. Ou -- Expression and characterization of the HCV NS2 protease / K.E. Reed and C.M. Rice -- Expression and characterization of HCV NS3 protease / E. D'Souza [and others] -- Expression and characterization of the HCV NS3 helicase domain / F. Preugschat [and others] -- Hepatitis C virus RNA-dependent RNA polymerase (NS5B polymerase) / C.H. Hagedorn -- Detection and molecular cloning of the extreme 3'-end of HCV / T. Tanaka and and K. Shimotohno -- In vitro stability of hepatitis C virus RNA / J.W.S. Fang -- Methods for the study of sequence-specific binding of proteins to the HCV RNA genome / L.M. Kaplan and R.T. Chung -- Role of immune response in HCV / M. Peters -- Determination of hepatitis C virus-specific CD4+ T-cell activity in PBMC / J.T. Gerlach, H. Diepolder and G.R. Pape -- Measurement of HCV-specific CD8+ cytotoxic T-cell activities in the peripheral blood by europium release assay / M. Imawari -- Determination of HCV-specific bulk CD8+ activity in liver / D.R. Nelson -- Characterization of HCV-specific cytotoxic T-lymphocytes from liver tissue / M.J. Koziel -- Production of human monoclonal antibodies to hepatitis C virus and their characterization / M.U. Mondelli and A. Cerino -- Specific detection of negative strand RNA of hepatitis C virus using chemical RNA modification / T. Gunji and K. Shimotohno -- Strand-specific rTth RT-PCR for the analysis of HCV replication / R.E. Lanford and D. Chavez -- Cell culture systems for the detection of HCV infection / Y.K. Shimizu and H. Yoshikura -- Replication and detection of hepatitis C virus in liver-derived cell lines / R.P. González-Peralta -- Primary human hepatocyte culture for the study of HCV / J.F. O'Connell [and others] -- A cultivation method for highly differentiated primary chimpanzee hepatocytes permissive for hepatitis C virus replication / R.E. Lanford and L. Estlack -- Construction of recombinant vaccinia virus expressing HCV genes / J.A. Feller -- Construction of recombinant adeno-associated virus (AAV) / M. Reiser and S. Zolotukhin -- Production of replication-deficient adenovirus recombinants / G.W.G. Wilkinson, C. Rickards and B.E. Clarke -- Generation of recombinant herpes simplex virus ampiicons / Z. Hong and A.D. Kwong -- Construction of recombinant sindbis-based expression vectors for the study of HCV genes and their products / B.D. Lindenbach, I. Frolov and C.M. Rice -- The chimpanzee model : contributions and considerations / E. Muchmore -- Liver transplantation as a model to study hepatitis C virus infection / H.M. Hassoba and T.L. Wright.

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In Hepatitis C Protocols, Johnson YN Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus infection and its disease, hepatitis C, which afflict an estimated 170 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV in liver tissue, to HCV genotyping and the detection and quantification of HCV in serum. In addition to these key protocols, the authoritative contributors also evaluate recombinant proteins in immunological test systems, including the identification of important antigenic epitopes, antibodies, and recombinant antigens. Molecular and immunological techniques to study this virus and its infection, as well as animal models and recombinant virus technology commonly used to express hepatitis C viral proteins, are also developed. Hepatitis C Protocols summarizes the state-of-the-art in today's research and understanding of the virology of hepatitis C virus. The methods and systems detailed in this book will play a key role in the development of more effective antivirals, in the development of vaccines, and in a deeper understanding of viral replication strategies, viron structure, and the mechanisms of viron assembly and release. A valuable new standard resource for all researchers and clinicians, Hepatitis C Protocols leads the way toward improved prevention, diagnosis, and treatment of viral hepatitis C.

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