Quantitative PCR protocols / edited by Bernd Kochanowski and Udo Reischl.

Contributor(s): Kochanowski, Bernd | Reischl, Udo
Material type: TextTextSeries: Methods in molecular medicine: 26.Publisher: Totowa, N.J. : Humana Press, ©1999Description: 1 online resource (x, 304 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592592623; 1592592627; 0585239789; 9780585239781; 1280830514; 9781280830518; 9786610830510; 6610830517Subject(s): Polymerase chain reaction -- Laboratory manuals | RNA | Methods | DNA | Polymerase Chain Reaction | Investigative Techniques | Nucleic Acid Amplification Techniques | Nucleic Acids | Nucleic Acids, Nucleotides, and Nucleosides | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Genetic Techniques | Chemicals and Drugs | Réaction en chaîne de la polymérase -- Manuels de laboratoire | SCIENCE -- Life Sciences -- Molecular Biology | Polymerase chain reaction | Polymerase kettingreactie | Wetenschappelijke technieken | Biowissenschaften | Aufsatzsammlung | Polymerase-Kettenreaktion | Quantitative Analyse | GenGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Quantitative PCR protocols.; Online version:: Quantitative PCR protocols.DDC classification: 572.8 LOC classification: QP606.D46 | Q36 1999Other classification: 35.75 | BIO 180f | CIT 972f Online resources: Click here to access online
Contents:
General principles of quantitative PCR / L. Raeymaekers-Effects of collection, processing, and storage on RNA detection and quantification / M. Holodniy-Quantitative rt-PCR / P.D. Siebert-Kinetic quantitative PCR vs end-point quantitative PCR with inteRNAl standard / O. Lantz [and others]-Comparison of competitive PCR and positive control-based PCR / F. Mallet-End-point titration-PCR for quantitation of cytomegalovirus DNA / J.K. Kulski-Analysis of amplified DNA molecules by capillary electrophoresis and laser induced fluorescence / M.J. Fasco-Competitor calibration and analysis of competitive amplified PCR products by high-performance liquid chromatography (hplc) / T. Kohler-Quantifying amplicons with elisa / O. Lantz, E. Bonney and Y. Taoufik-Competitive PCR quantitation utilizing a microtiter plate based format for the detection of PCR products / B. Kochanowski and W. Jilg-Competitive and differential rt-PCR (cd-rt-PCR) for measurement of normalized gene expression using antisense competitors / E. de Kant-Amplified assay for specific dual-labeled DNA using the coagulation cascade (edna-elca) / G.J. Doellgast [and others]-Quantitative PCR with inteRNAl standardization and ola-elisa product analysis for the p53 tumor suppressor gene / M. Hahn and A. Pingoud-Quantitative analysis of human DNA sequences by PCR and solid-phase minisequencing / A. Suomalainen and A.C. Syvanen-High resolution PCR quantitation by amplisensor assay / C.N. Wang-Construction of polycompetitors for competitive PCR / D.B. Corry and R.M. Locksley-Tailed rt-PCR for the quantitation of chloramphenicol acetyl transferase (cat)mRNA / M.C. Knuchel and A.A. Ansari-A stochastic PCR approach for RNA quantification in multiple samples / A. Puntschart and M. Vogt-Quantitation of mRNA species by rt-PCR on total mRNA population with nonradioactive probes / S. Herblot, B. Rousseau and J. Bonnet.
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions.
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Includes bibliographical references and index.

General principles of quantitative PCR / L. Raeymaekers-Effects of collection, processing, and storage on RNA detection and quantification / M. Holodniy-Quantitative rt-PCR / P.D. Siebert-Kinetic quantitative PCR vs end-point quantitative PCR with inteRNAl standard / O. Lantz [and others]-Comparison of competitive PCR and positive control-based PCR / F. Mallet-End-point titration-PCR for quantitation of cytomegalovirus DNA / J.K. Kulski-Analysis of amplified DNA molecules by capillary electrophoresis and laser induced fluorescence / M.J. Fasco-Competitor calibration and analysis of competitive amplified PCR products by high-performance liquid chromatography (hplc) / T. Kohler-Quantifying amplicons with elisa / O. Lantz, E. Bonney and Y. Taoufik-Competitive PCR quantitation utilizing a microtiter plate based format for the detection of PCR products / B. Kochanowski and W. Jilg-Competitive and differential rt-PCR (cd-rt-PCR) for measurement of normalized gene expression using antisense competitors / E. de Kant-Amplified assay for specific dual-labeled DNA using the coagulation cascade (edna-elca) / G.J. Doellgast [and others]-Quantitative PCR with inteRNAl standardization and ola-elisa product analysis for the p53 tumor suppressor gene / M. Hahn and A. Pingoud-Quantitative analysis of human DNA sequences by PCR and solid-phase minisequencing / A. Suomalainen and A.C. Syvanen-High resolution PCR quantitation by amplisensor assay / C.N. Wang-Construction of polycompetitors for competitive PCR / D.B. Corry and R.M. Locksley-Tailed rt-PCR for the quantitation of chloramphenicol acetyl transferase (cat)mRNA / M.C. Knuchel and A.A. Ansari-A stochastic PCR approach for RNA quantification in multiple samples / A. Puntschart and M. Vogt-Quantitation of mRNA species by rt-PCR on total mRNA population with nonradioactive probes / S. Herblot, B. Rousseau and J. Bonnet.

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In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions.

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