Phospholipid signaling protocols / edited by Ian M. Bird.
Contributor(s): Bird, Ian MMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 105.Publisher: Totowa, N.J. : Humana Press, ©1998Description: 1 online resource (xii, 380 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 0896034917; 9780896034914; 9781592592555; 0585242208; 9780585242200; 1592592554; 1280830484; 9781280830488; 9786610830480; 6610830487Subject(s): Phospholipids -- Research -- Methodology | Membrane lipids -- Research -- Methodology | Cellular signal transduction -- Research -- Methodology | Second messengers (Biochemistry) -- Research -- Methodology | Phospholipids -- physiology | Research Design | Second Messenger Systems -- physiology | SCIENCE -- Life Sciences -- Biochemistry | Cellular signal transduction -- Research -- Methodology | Second messengers (Biochemistry) -- Research -- Methodology | Fosfolipiden | Signalen | Wetenschappelijke techniekenGenre/Form: Electronic books. Additional physical formats: Print version:: Phospholipid signaling protocols.DDC classification: 572/.57 LOC classification: QP752.P53 | P464 1998ebOther classification: 42.15 Online resources: Click here to access online
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Includes bibliographical references and index.
Phosphoinositidase C activation assay. 1, Cell labeling, stimulation, and recovery of cellular [³H]Phosphoinositides and [³H]phosphoinositols / Ian M. Bird -- Phosphoinositidase C activation assay. 2, Simple analysis of recovered cellular phosphoinositides and phosphoinositols / Ian M. Bird -- Phosphoinositidase C activation assay. 3, HPLC analysis of cellular phosphoinositides and phosphoinositols / Ian M. Bird -- Measurement of phosphoinositols and phosphoinositides using radio high-performance liquid chromatography flow detection / Lubo Zhang, Iain L. Buxton -- Preparation of [³H]phosphoinositol standards and conversion of [³H]phosphoinositides to [³H]phosphoinositols / Ian M. Bird -- Inositol 1,4,5-trisphosphate mass assay / Lubo Zhang -- Measurement of cellular diacylglycerol content / Wendy Bollinger Bollag, Richard D. Griner -- Phosphatidylinositol 4-kinases : assays and product analysis / Rachel E. Meyers, Lewis C. Cantley -- Phosphatidylinositol(3,4,5)trisphosphate (Ptdins(3,4,5)P₃) mass measurement using a radioligand displacement assay / Jeroen Kaay, Pete J. Cullen, C. Peter Downes -- Detection of phosphatidylinositol-4-phosphate 5-kinase activity using thin-layer chromatography / Gregory J. Parker, Joost C. Loijens, Richard A. Anderson -- Determination of phospholipase C-or phospholipase D-catalyzed phosphatidylcholine hydrolysis / Michael J. Wakelam, Trevor R. Pettitt -- Measurement of phospholipase D activity / Wendy Bollinger Bollag -- Monitoring of phospholipase A₂ activation in cultured cells using tritiated arachidonic acid / Sandie I. Briand, Sylvie G. Bernier, Gaétan Guillemette -- Assay of cellular diacylglycerol and monoacylglycerol lipases / Bryan A. Wolf -- Isotopic efflux studies as indices of phospholipase activation / Jacob Vadakekalam, Stewart Metz -- Extraction and measurements of prostanoids and leukotrienes by radioimmunoassays / Bruno Battistini, Serge Picard, Pierre Borgeat, Pierre Sirois -- Measurements of prostanoids, leukotrienes, and isoprostanes by enzyme immunoassays / Bruno Battistini, Serge Picard, Pierre Borgeat, Pierre Sirois -- Measurements of arachidonic acid metabolites derived from the lipoxygenase pathways by high-pressure liquid chromatography / Pierre Borgeat, Serge Picard, Bruno Battistini, Pierre Sirois -- Measurement of sphingomyelin and ceramide cellular levels after sphingomyelinase-mediated sphingomyelin hydrolysis / Pino Santana, Luisa F. Fanjul, C.M. Ruiz Galarreta -- Sphingomyelin and ceramide mass assay / Pino Santana, C.M. Ruiz Galarreta, Luisa F. Fanjul -- Sphingosine kinase : assay and product analysis / Ana Olivera, Sarah Spiegel -- Analytical methods and steps to sample preparation for determination of molecular species of fatty acids / Sanda Clejan -- HPLC analytical methods for the separation of molecular species of fatty acids in diacylglycerol and cellular phospholipids / Sanda Clejan -- Analysis of molecular species of cellular sphingomyelins and ceramides / Sanda Clejan -- Solubilization and assay of cellular and tissue protein / Cynthia E. Shaw -- Western immunoblot analysis / Cynthia E. Shaw, Jing Zheng -- Immunohistochemistry and immunocytochemistry / Jing Zheng -- Extraction of cellular and tissue RNA / Daniel S. Millican, Ian M. Bird -- Size separation and quantification of mRNA by Northern analysis / Ian M. Bird -- Preparation of single-stranded antisense cDNA probes by asymmetric PCR / Daniel S. Millican, Ian M. Bird -- Optimization of a reverse transcription-polymerase chain reaction (RT-PCR) mass assay for low-abundance mRNA / Jacqueline M. Cale, Cynthia E. Shaw, Ian M. Bird.
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In Phospholipid Signaling Protocols, state-of-the-art techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation are detailed by experts intimately experienced in their use. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites, including phosphoinositols, choline metabolites, and fatty acid metabolites. In addition, there is extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA, as well as a description of their localization by immunohistochemistry and immunocytochemistry. Phospholipid Signaling Protocols offers a wide-ranging collection of cutting-edge techniques for the study of signal transduction through phospholipid intermediates and their metabolites. The book is an indispensable reference for both the newcomer and the experienced researcher seeking to expand knowledge of these critical pathways, and strongly complements its companion volumes-R.A.J. Challiss' Receptor Signal Transduction Protocols, D. Bar-Sagi's, Transmembrane Signaling Protocols, and D.A. Kendall and S.J. Hill's Signal Transduction Protocols-in building a unique library of tried-and-tested protocols relating to the ever expanding signal transduction field.