Cytomegalovirus protocols / edited by John Sinclair.

Contributor(s): Sinclair, John, Ph. D
Material type: TextTextSeries: Methods in molecular medicine: 33.Publisher: Totowa, N.J. : Humana Press, ©2000Description: 1 online resource (x, 182 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592592449; 1592592449; 0585235678; 9780585235677; 0896037495; 9780896037496Subject(s): Cytomegalovirus infections -- Laboratory manuals | Cytomegaloviruses -- Laboratory manuals | Cytomegalovirus -- isolation & purification | Cytomegalovirus -- genetics | Cytomegalovirus Infections -- genetics | Genetic Techniques | MEDICAL -- Microbiology | Cytomegalovirus infections | Cytomegaloviruses | Wetenschappelijke techniekenGenre/Form: Laboratory Manual. | Electronic books. | Handbooks and manuals. Additional physical formats: Print version:: Cytomegalovirus protocols.DDC classification: 616/.0194 LOC classification: QR201.C94 | C94 2000ebOnline resources: Click here to access online
Contents:
Immunological Methods for the Detection of Human Cytomegalovirus / Andrew D. Yurochko, Eng-Shang Huang -- Identification of Immunoreactive Viral Proteins / Walter Hinderer, Bodo Plachter, Rolf Vornhagen -- Analysis of Human Cytomegalovirus Using the Polymerase Chain Reaction / Marc Mendelson -- Analysis of Cytomegalovirus Ligands, Receptors, and the Entry Pathway / Teresa Compton -- Analysis of Cytomegalovirus Gene Expression by Transfection / Anamaris M. Colberg-Poley, Vera E. Soltero, Richard-Fabian Schumacher -- Analysis of Protein-Protein Interactions During Cytomegalovirus Infection / Richard C. Caswell -- Analysis of Protein-DNA Interactions During Cytomegalovirus Infection / Stefan Floss, Thomas Stamminger -- Analysis of Cytotoxic T-Cell Responses to Human Cytomegalovirus Infection / Mark R. Wills, J.G. Patrick Sissons -- Methods in Anti-HCMV Research / Johan Neyts, Graciela Andrei, Robert Snoeck, Astrid Meerbach, Erik De Clercq -- Construction of Recombinant Human Cytomegalovirus / Richard F. Greaves.
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: In Cytomegalovirus Protocols (CMV), John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host. Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a powerful collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in the pathogenesis of human infectious diseases as well as their emergent therapies.
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Includes bibliographical references and index.

Immunological Methods for the Detection of Human Cytomegalovirus / Andrew D. Yurochko, Eng-Shang Huang -- Identification of Immunoreactive Viral Proteins / Walter Hinderer, Bodo Plachter, Rolf Vornhagen -- Analysis of Human Cytomegalovirus Using the Polymerase Chain Reaction / Marc Mendelson -- Analysis of Cytomegalovirus Ligands, Receptors, and the Entry Pathway / Teresa Compton -- Analysis of Cytomegalovirus Gene Expression by Transfection / Anamaris M. Colberg-Poley, Vera E. Soltero, Richard-Fabian Schumacher -- Analysis of Protein-Protein Interactions During Cytomegalovirus Infection / Richard C. Caswell -- Analysis of Protein-DNA Interactions During Cytomegalovirus Infection / Stefan Floss, Thomas Stamminger -- Analysis of Cytotoxic T-Cell Responses to Human Cytomegalovirus Infection / Mark R. Wills, J.G. Patrick Sissons -- Methods in Anti-HCMV Research / Johan Neyts, Graciela Andrei, Robert Snoeck, Astrid Meerbach, Erik De Clercq -- Construction of Recombinant Human Cytomegalovirus / Richard F. Greaves.

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In Cytomegalovirus Protocols (CMV), John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host. Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a powerful collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in the pathogenesis of human infectious diseases as well as their emergent therapies.

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