Arabidopsis protocols / edited by José M. Martínez-Zapater and Julio Salinas.

Contributor(s): Martínez-Zapater, José M | Salinas, Julio
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 82.Publisher: Totowa, N.J. : Humana Press, ©1998Description: 1 online resource (xvi, 440 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781592592685; 1592592686; 0585282420; 9780585282428Subject(s): Arabidopsis -- Laboratory manuals | Arabidopsis -- Molecular aspects -- Laboratory manuals | Arabidopsis | NATURE -- Plants -- General | Arabidopsis | Dicotyledoneae | Dicotyledons | brassicaceae | genen | genes | genetica | genetics | genetische variatie | genetic variation | immunogenetica | immunogenetics | moleculaire biologie | molecular biology | genkartering | gene mapping | genexpressie | gene expression | protocollen | protocols | Plant Molecular Biology | Biological Techniques | Genetic Engineering | Molecular Genetics | Nucleic Acids | Moleculaire plantenbiologie | Biologische technieken | Genetische modificatie | Moleculaire geneticaGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Arabidopsis protocols.DDC classification: 583/.64 LOC classification: QK495.C9 | A735 1998ebOnline resources: Click here to access online
Contents:
Growth of plants and preservation of seeds / Randy Scholl, Luz Rivero-Lepickas, Deborah Crist -- Sterile techniques in Arabidopsis / Peter McCourt, Kallie Keith -- Control of pests and diseases of Arabidopsis / Mary Anderson -- Establishment and maintenance of cell suspension cultures / Jaideep Mathur, Csaba Koncz -- Callus culture and regeneration / Jaideep Mathur, Csaba Koncz -- Protoplast isolation, culture, and regeneration / Jaideep Mathur, Csaba Koncz -- Preparation of physiologically active chloroplasts from Arabidopsis / Ljerka Kunst -- Purification of mitochondria from Arabidopsis / Mathieu Klein, Stefan Binder, Axel Brennicke -- Preparation of DNA from Arabidopsis / Jianming Li, Joanne Chory -- High molecular weight DNA extraction from arabidopsis / David Bouchez, Christine Camilleri -- Chloroplast DNA isolation / George S. Mourad -- Purification of mitochondrial DNA from green tissues of Arabidopsis / Mathieu Klein, Rudolf Hiesel, Charles André, Axel Brennicke -- Preparation of RNA / Clifford D. Carpenter, Anne E. Simon -- Seed mutagenesis of arabidopsis / Jonathan Lightner, Timothy Caspar -- Genetic analysis / Maarten Koornneef, Carlos Alonso-Blanco, Piet Stam -- Cytogenetic analysis of Arabidopsis / John S. Heslop-Harrison -- PCR-based identification of T-DNA insertion mutants / Rodney G. Winkler, Kenneth A. Feldmann -- The use of recombinant inbred lines (RILs) for genetic mapping / Carlos Alonso-Blanco, Maarten Koornneef, Piet Stam -- AFLP fingerprinting of Arabidopsis / Pieter Vos -- Building a high-density genetic map using the AFLP technology / Martin T. Kuiper -- Use of cleaved amplified polymorphic sequences (CAPS) as genetic markers in Arabidopsis thaliana / Jane Glazebrook, Eliana Drenkarci, Daphne Preuss, Frederick M. Ausubel -- Mapping mutations with ARMS / Anton R. Schiiffner -- Mapping cloned sequences on YACs / Francis D. Agyare, Gus Lagos, Deval Lashkari, Ronald W. Davis, Bertrand Lemieux -- Transient gene expression in protoplasts of Arabidopsis thaliana / Steffen Abel, Athanasios Theologis -- Transient expression of foreign genes in tissues of Arabidopsis thaliana by bombardment-mediated transformation / Motoaki Seki, Asako Lida, Hiromichi Morikawa -- Root transformation by Agrobacterium tumefaciens / Annette C. Vergunst, Ellen C. Waal, Paul J. Hooykaas -- Transformation of Arabidopsis thaliana C24 leaf discs by Agrobacterium tumefaciens / Eric Graaff, Paul J.J. Hooykaas -- In planta agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration / Nicole Bechtold, Georges Pelletier -- PEG-mediated protoplast transformation with naked DNA / Jaideep Mathur, Csaba Koncz -- Cloning genes of Arabidopsis thaliana by chromosome walking / Jeffrey Leung, Jérôme Giraudat -- Chromosome landing using an AFLP-based strategy / Ann Van Gysel, Gerda Cnops, Peter Breyne, Marc Van Montagu, Maria Teresa Cervera -- Transposon tagging with AC/Ds in -- arabidopsis / Deborah Long, George Coupland -- Transposon tagging with the EN-I system / Andy Pereira, Mark G. Aarts -- Cloning genes from T-DNA tagged mutants / Brian P. Dilkes, Kenneth A. Feldmann -- In situ hybridization / Gary N. Drews -- Whole-mount in situ hybridization in plants / Janice de Engler, Marc Van Montagu, Gilbert Engler -- Bacterial and coelenterate luciferases as reporter genes in plant cells / William H.R Langridge, Aladar A. Szalay -- [Beta]-glucuronidase enzyme histochemistry on semithin sections of plastic-embedded Arabidopsis explants / Marc De Block, Mieke Van Lijsebettens -- In situ hybridization to RNA in whole Arabidopsis plants / Qingzhong Kong, Anne E. Simon -- In vivo footprinting in Arabidopsis / Anna-Lisa Paul, Robert J. Ferl.
Summary: N Arabidopsis Protocols internationally recognized experts offer a comprehensive collection of significant new methods for gene mapping, cloning, and reverse genetics, as well as updated standard protocols for work in Arabidopsis genetics and molecular biology. These time-tested methods range from techniques for the successful growing of Arabidopsis to strategies for gene cloning. The sections on genetics, transformation, and gene expression analysis will be especially helpful to scientists involved in mutant analysis or in producing and analyzing transgenic plants. In many cases, the protocols can also be applied to other plant species, with minor adjustments. Serving both experienced researchers and beginners in the field, Arabidopsis Protocols will immediately become the standard reference for laboratories working with Arabidopsis, the plant of choice to approach many fundamental questions in plant biology.
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Includes bibliographical references and index.

Growth of plants and preservation of seeds / Randy Scholl, Luz Rivero-Lepickas, Deborah Crist -- Sterile techniques in Arabidopsis / Peter McCourt, Kallie Keith -- Control of pests and diseases of Arabidopsis / Mary Anderson -- Establishment and maintenance of cell suspension cultures / Jaideep Mathur, Csaba Koncz -- Callus culture and regeneration / Jaideep Mathur, Csaba Koncz -- Protoplast isolation, culture, and regeneration / Jaideep Mathur, Csaba Koncz -- Preparation of physiologically active chloroplasts from Arabidopsis / Ljerka Kunst -- Purification of mitochondria from Arabidopsis / Mathieu Klein, Stefan Binder, Axel Brennicke -- Preparation of DNA from Arabidopsis / Jianming Li, Joanne Chory -- High molecular weight DNA extraction from arabidopsis / David Bouchez, Christine Camilleri -- Chloroplast DNA isolation / George S. Mourad -- Purification of mitochondrial DNA from green tissues of Arabidopsis / Mathieu Klein, Rudolf Hiesel, Charles André, Axel Brennicke -- Preparation of RNA / Clifford D. Carpenter, Anne E. Simon -- Seed mutagenesis of arabidopsis / Jonathan Lightner, Timothy Caspar -- Genetic analysis / Maarten Koornneef, Carlos Alonso-Blanco, Piet Stam -- Cytogenetic analysis of Arabidopsis / John S. Heslop-Harrison -- PCR-based identification of T-DNA insertion mutants / Rodney G. Winkler, Kenneth A. Feldmann -- The use of recombinant inbred lines (RILs) for genetic mapping / Carlos Alonso-Blanco, Maarten Koornneef, Piet Stam -- AFLP fingerprinting of Arabidopsis / Pieter Vos -- Building a high-density genetic map using the AFLP technology / Martin T. Kuiper -- Use of cleaved amplified polymorphic sequences (CAPS) as genetic markers in Arabidopsis thaliana / Jane Glazebrook, Eliana Drenkarci, Daphne Preuss, Frederick M. Ausubel -- Mapping mutations with ARMS / Anton R. Schiiffner -- Mapping cloned sequences on YACs / Francis D. Agyare, Gus Lagos, Deval Lashkari, Ronald W. Davis, Bertrand Lemieux -- Transient gene expression in protoplasts of Arabidopsis thaliana / Steffen Abel, Athanasios Theologis -- Transient expression of foreign genes in tissues of Arabidopsis thaliana by bombardment-mediated transformation / Motoaki Seki, Asako Lida, Hiromichi Morikawa -- Root transformation by Agrobacterium tumefaciens / Annette C. Vergunst, Ellen C. Waal, Paul J. Hooykaas -- Transformation of Arabidopsis thaliana C24 leaf discs by Agrobacterium tumefaciens / Eric Graaff, Paul J.J. Hooykaas -- In planta agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration / Nicole Bechtold, Georges Pelletier -- PEG-mediated protoplast transformation with naked DNA / Jaideep Mathur, Csaba Koncz -- Cloning genes of Arabidopsis thaliana by chromosome walking / Jeffrey Leung, Jérôme Giraudat -- Chromosome landing using an AFLP-based strategy / Ann Van Gysel, Gerda Cnops, Peter Breyne, Marc Van Montagu, Maria Teresa Cervera -- Transposon tagging with AC/Ds in -- arabidopsis / Deborah Long, George Coupland -- Transposon tagging with the EN-I system / Andy Pereira, Mark G. Aarts -- Cloning genes from T-DNA tagged mutants / Brian P. Dilkes, Kenneth A. Feldmann -- In situ hybridization / Gary N. Drews -- Whole-mount in situ hybridization in plants / Janice de Engler, Marc Van Montagu, Gilbert Engler -- Bacterial and coelenterate luciferases as reporter genes in plant cells / William H.R Langridge, Aladar A. Szalay -- [Beta]-glucuronidase enzyme histochemistry on semithin sections of plastic-embedded Arabidopsis explants / Marc De Block, Mieke Van Lijsebettens -- In situ hybridization to RNA in whole Arabidopsis plants / Qingzhong Kong, Anne E. Simon -- In vivo footprinting in Arabidopsis / Anna-Lisa Paul, Robert J. Ferl.

Print version record.

N Arabidopsis Protocols internationally recognized experts offer a comprehensive collection of significant new methods for gene mapping, cloning, and reverse genetics, as well as updated standard protocols for work in Arabidopsis genetics and molecular biology. These time-tested methods range from techniques for the successful growing of Arabidopsis to strategies for gene cloning. The sections on genetics, transformation, and gene expression analysis will be especially helpful to scientists involved in mutant analysis or in producing and analyzing transgenic plants. In many cases, the protocols can also be applied to other plant species, with minor adjustments. Serving both experienced researchers and beginners in the field, Arabidopsis Protocols will immediately become the standard reference for laboratories working with Arabidopsis, the plant of choice to approach many fundamental questions in plant biology.

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