The nucleic acid protocols handbook / edited by Ralph Rapley.

Contributor(s): Rapley, Ralph
Material type: TextTextPublisher: Totowa, N.J. : Humana Press, ©2000Description: 1 online resource (xxii, 1050 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592590384; 1592590381; 0585399387; 9780585399386Subject(s): Nucleic acids -- Laboratory manuals | Polymerase chain reaction -- Laboratory manuals | Gene mapping -- Laboratory manuals | Molecular genetics -- Laboratory manuals | Nucleic Acids -- isolation & purification | Chromosome Mapping -- methods | Molecular Biology -- methods | Polymerase Chain Reaction -- methods | SCIENCE -- Life Sciences -- Molecular Biology | Gene mapping | Molecular genetics | Nucleic acids | Polymerase chain reactionGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Nucleic acid protocols handbook.DDC classification: 572.8 LOC classification: QP620 | .N7987 2000Online resources: Click here to access online
Contents:
pt. 1. Nucleic acid extraction -- pt. 2. Basic separation and analysis of DNA -- pt. 3. Probe design, synthesis, and labeling -- pt. 4. RNA analyis techniques -- pt. 5. Gene library construction and screening -- pt. 6. DNA screening -- pt. 7. Basic polymerase chain reaction methods -- pt. 8. Analyzing genes, mutations, and protein interactions -- pt. 9. Mutagenesis, transcription, and translation in vitro pt. 10. Gene localization, mapping in situ, and bioinformatics.
Summary: The Nucleic Acid Protocols Handbook is today's most comprehensive up-to-date treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology series, providing an introduction to the scientific basis of each technique, a complete listing of all the materials and reagents needed, and clear step-by-step instructions to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success in the lab. The authord are well-experienced masters of the techniques described, often having perfected and optimized them in their own laboratories. In The Nucleic Acid Protocols Handbook, Ralph Rapley has brought together the widest-ranging, one-volume collection of classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids. Eminently practical and reproducible, these proven methods will help both experienced researchers and those entering the field attain the laboratory mastery needed-not only to deepen understanding of biological processes at the molecular level, but also to begin realizing the biotechnological bounty so clearly in prospect.
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Includes bibliographical references and index.

The Nucleic Acid Protocols Handbook is today's most comprehensive up-to-date treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology series, providing an introduction to the scientific basis of each technique, a complete listing of all the materials and reagents needed, and clear step-by-step instructions to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success in the lab. The authord are well-experienced masters of the techniques described, often having perfected and optimized them in their own laboratories. In The Nucleic Acid Protocols Handbook, Ralph Rapley has brought together the widest-ranging, one-volume collection of classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids. Eminently practical and reproducible, these proven methods will help both experienced researchers and those entering the field attain the laboratory mastery needed-not only to deepen understanding of biological processes at the molecular level, but also to begin realizing the biotechnological bounty so clearly in prospect.

Print version record.

pt. 1. Nucleic acid extraction -- pt. 2. Basic separation and analysis of DNA -- pt. 3. Probe design, synthesis, and labeling -- pt. 4. RNA analyis techniques -- pt. 5. Gene library construction and screening -- pt. 6. DNA screening -- pt. 7. Basic polymerase chain reaction methods -- pt. 8. Analyzing genes, mutations, and protein interactions -- pt. 9. Mutagenesis, transcription, and translation in vitro pt. 10. Gene localization, mapping in situ, and bioinformatics.

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