Natural killer cell protocols : cellular and molecular methods / edited by Kerry S. Campbell and Marco Colonna.

Contributor(s): Campbell, Kerry S | Colonna, M. (Marco), M.D
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 121.Publisher: Totowa, N.J. : Humana Press, ©2000Description: 1 online resource (xvii, 398 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592590445; 0585400652; 9780585400655; 1592590446; 9786610820368; 6610820368; 1280820365; 9781280820366Subject(s): Killer cells -- Laboratory manuals | Killer Cells, Natural | Cytotoxicity Tests, Immunologic | Methods | Toxicity Tests | Immunologic Tests | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Laboratory Techniques and Procedures | Immunologic Techniques | Leukocytes, Mononuclear | Leukocytes | Diagnosis | Immune System | Blood Cells | Cells | Hemic and Immune Systems | Blood | Anatomy | Lymphocytes | Investigative Techniques | MEDICAL -- Immunology | Killer cells | Wetenschappelijke techniekenGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Natural killer cell protocols.DDC classification: 616.07/9 LOC classification: QR185.8.K54 | N3676 2000Online resources: Click here to access online
Contents:
Cloning Human Natural Killer Cells / Marina Cella, Marco Colonna -- Generation of Short-Term Murine Natural Killer Cell Clones to Analyze Ly49 Gene Expression / Werner Held, Bente Lowin-Kropf, David H. Raulet -- Cloning and Culturing of Fetal Mouse Natural Killer Cells / Colin G. Brooks -- Techniques for Studying Development of Human Natural Killer Cells and T Cells / Hergen Spits, Pieter Res, Ana-Cristina Jaleco -- Murine Natural Killer Cell Cloning from Fetal Thymic Organ Cultures / Zuhair K. Ballas, Wendy L. Rasmussen -- Generation of HLA Class I Transfected Target Cell Lines / Jenny E. Gumperz -- Murine Natural Killer Cells and Hybrid Resistance to Hemopoietic Cells In Vivo / Michael Bennett [and others] -- Hybrid Resistance by Mouse NK Cells In Vitro / Michael Bennett [and others] -- Use of Activated Natural Killer Cells for Tumor Immunotherapy in Mouse and Human / Per H. Basse, Theresa L. Whiteside, Ronald B. Herberman -- Methods for In Vivo Analyses of Natural Killer (NK) Cells / Per H. Basse, William H. Chambers, Simon M. Watkins -- Assay for the Transendothelial Migration of Human Natural Killer Cells / Miriam E. Berman, William A. Muller -- Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays / Richard C. Duke -- Methods for Detecting Lytic Granules in Natural Killer Cells / Alison Darmon, Lesley J. Page, Gillian M. Griffiths -- Fluorogenic Substrates as Detectors of Caspase Activity During Natural Killer Cell-Induced Apoptosis / Marek Los [and others] -- Techniques For Studying Murine Natural Killer Cells in Defense Against Viral Infection / Anthony A. Scalzo, Helen E. Farrell, Gunasegaran Karupiah -- Assays for Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Reverse ADCC (Redirected Cytotoxicity) in Human Natural Killer Cells / Bice Perussia, Matthew J. Loza -- Multiple Color Immunofluorescence for Cytokine Detection at the Single Cell Level / Bice Perussia, Matthew J. Loza, Jeffrey S. Faust -- Generation of Antibodies to Cell Surface Markers on Mature Natural Killer Cells / Michael Bennett [and others] -- Human Single-Chain Fv Fragments Specific for Natural Killer Cell Receptors from Phage Display Libraries / Barbara Uchanska-Ziegler [and others] -- Binding of Soluble KIR-Fc Fusion Proteins to HLA Class I / Christine C. Winter, Eric O. Long -- Genetic Analysis of a Highly Homologous Gene Family : The Killer Cell Immunoglobulin-Like Receptors / Michael J. Wilson, Michaela Torkar, John Trowsdale -- Use of Vaccinia Virus for Functional Gene Transfer in Natural Killer Cells / Eric O. Long, Sumati Rajagopalan -- Use of cDNA Library Expression Cloning to Identify Components of Heterodimeric Receptor Complexes / Chiwen Chang, Lewis L. Lanier -- Functional Analysis of Natural Killer Cell Receptors in the RNK-16 Rat Leukemic Cell Line / James C. Ryan, Erene C. Niemi, Mary C. Nakamura -- Use of Mutant Jurkat T Cell Lines to Define Human Natural Killer Cell Surface Receptor Signaling Pathways / Bryce A. Binstadt [and others] -- Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis / Salvatore Valitutti, Mark Dessing -- BlAcore Analysis to Test Phosphopeptide-SH2 Domain Interactions / Frederic Vely, Alain Trautmann, Eric Vivier -- Analysis and Use of Mutant Mice Exhibiting Natural Killer Cell Defects / Toru Miyazaki, Kerry S. Campbell -- Isolation and Analysis of Natural Killer Cells in Chickens / Thomas W.F. Gobel -- Analyzing Nonspecific Cytotoxic Cells in Fish / Sandra Ristow, Donald L. Evans, Liliana Jaso-Friedmann -- Natural Killer Cells and Immunotoxicology / Albert E. Munson, Kathleen E. Philips.
Action note: digitized 2010 committed to preserveSummary: In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.
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Includes bibliographical references and index.

Cloning Human Natural Killer Cells / Marina Cella, Marco Colonna -- Generation of Short-Term Murine Natural Killer Cell Clones to Analyze Ly49 Gene Expression / Werner Held, Bente Lowin-Kropf, David H. Raulet -- Cloning and Culturing of Fetal Mouse Natural Killer Cells / Colin G. Brooks -- Techniques for Studying Development of Human Natural Killer Cells and T Cells / Hergen Spits, Pieter Res, Ana-Cristina Jaleco -- Murine Natural Killer Cell Cloning from Fetal Thymic Organ Cultures / Zuhair K. Ballas, Wendy L. Rasmussen -- Generation of HLA Class I Transfected Target Cell Lines / Jenny E. Gumperz -- Murine Natural Killer Cells and Hybrid Resistance to Hemopoietic Cells In Vivo / Michael Bennett [and others] -- Hybrid Resistance by Mouse NK Cells In Vitro / Michael Bennett [and others] -- Use of Activated Natural Killer Cells for Tumor Immunotherapy in Mouse and Human / Per H. Basse, Theresa L. Whiteside, Ronald B. Herberman -- Methods for In Vivo Analyses of Natural Killer (NK) Cells / Per H. Basse, William H. Chambers, Simon M. Watkins -- Assay for the Transendothelial Migration of Human Natural Killer Cells / Miriam E. Berman, William A. Muller -- Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays / Richard C. Duke -- Methods for Detecting Lytic Granules in Natural Killer Cells / Alison Darmon, Lesley J. Page, Gillian M. Griffiths -- Fluorogenic Substrates as Detectors of Caspase Activity During Natural Killer Cell-Induced Apoptosis / Marek Los [and others] -- Techniques For Studying Murine Natural Killer Cells in Defense Against Viral Infection / Anthony A. Scalzo, Helen E. Farrell, Gunasegaran Karupiah -- Assays for Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Reverse ADCC (Redirected Cytotoxicity) in Human Natural Killer Cells / Bice Perussia, Matthew J. Loza -- Multiple Color Immunofluorescence for Cytokine Detection at the Single Cell Level / Bice Perussia, Matthew J. Loza, Jeffrey S. Faust -- Generation of Antibodies to Cell Surface Markers on Mature Natural Killer Cells / Michael Bennett [and others] -- Human Single-Chain Fv Fragments Specific for Natural Killer Cell Receptors from Phage Display Libraries / Barbara Uchanska-Ziegler [and others] -- Binding of Soluble KIR-Fc Fusion Proteins to HLA Class I / Christine C. Winter, Eric O. Long -- Genetic Analysis of a Highly Homologous Gene Family : The Killer Cell Immunoglobulin-Like Receptors / Michael J. Wilson, Michaela Torkar, John Trowsdale -- Use of Vaccinia Virus for Functional Gene Transfer in Natural Killer Cells / Eric O. Long, Sumati Rajagopalan -- Use of cDNA Library Expression Cloning to Identify Components of Heterodimeric Receptor Complexes / Chiwen Chang, Lewis L. Lanier -- Functional Analysis of Natural Killer Cell Receptors in the RNK-16 Rat Leukemic Cell Line / James C. Ryan, Erene C. Niemi, Mary C. Nakamura -- Use of Mutant Jurkat T Cell Lines to Define Human Natural Killer Cell Surface Receptor Signaling Pathways / Bryce A. Binstadt [and others] -- Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis / Salvatore Valitutti, Mark Dessing -- BlAcore Analysis to Test Phosphopeptide-SH2 Domain Interactions / Frederic Vely, Alain Trautmann, Eric Vivier -- Analysis and Use of Mutant Mice Exhibiting Natural Killer Cell Defects / Toru Miyazaki, Kerry S. Campbell -- Isolation and Analysis of Natural Killer Cells in Chickens / Thomas W.F. Gobel -- Analyzing Nonspecific Cytotoxic Cells in Fish / Sandra Ristow, Donald L. Evans, Liliana Jaso-Friedmann -- Natural Killer Cells and Immunotoxicology / Albert E. Munson, Kathleen E. Philips.

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In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.

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