Protocols for neural cell culture / edited by Sergey Fedoroff and Arleen Richardson.

Contributor(s): Fedoroff, Sergey | Richardson, Arleen
Material type: TextTextSeries: Springer protocols (Series): Publisher: Totowa, N.J. : Humana Press, ©2001Edition: 3rd edDescription: 1 online resource (xxii, 362 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592592074; 1592592074; 0585403171; 9780585403175Subject(s): Nerve tissue -- Cultures and culture media -- Laboratory manuals | Neurons -- Growth -- Laboratory manuals | Nerve Tissue -- cytology | Tissue Culture -- methods | Neurons -- cytology | Culture Techniques -- methods | Tissu nerveux -- Cultures et milieux de culture | Neurones -- Croissance | MEDICAL -- Histology | Nerve tissue -- Cultures and culture media | Neurons -- Growth | Neuronen | Celkweek | LaboratoriumonderzoekGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Protocols for neural cell culture.DDC classification: 611/.0188 LOC classification: QP356.25 | .P765 2001Other classification: 42.16 Online resources: Click here to access online
Contents:
In vitro assays for axonal growth and targeting / Valérie Castellani and Jürgen Bolz -- Interface organotypic hippocampal slice cultures / Dominique Muller [and others] -- Slice cultures for study of microglia / Carol A. Colton, Meggan Czapiga, and Toby N. Behar -- Microexplant cultures of the cerebellum / Bernard Rogister and Gustave Moonen -- Construction and use of compartmented cultures for studies of cell biology of neurons / Robert B. Campenot and Grace Martin -- Chick spinal somatic motoneurons in culture / Bernhard H.J. Juurlink -- Primary cultures of sympathetic ganglia / Mary I. Johnson -- Primary cell cultures for the study of myelination / Mary I. Johnson, Richard P. Bunge, and Patrick M. Wood -- Preparation of astrocyte, oligodendrocyte, and microglia cultures for primary rat cerebral cultures / Ruth Cole and Jean de Vellis -- Culture of glial cells from human brain biopsies / V. Wee Yong and Jack P. Antel.
Cultures of astroglia and microglia from primary cultures of mouse neopallium / Sergey Fedoroff and Arleen Richardson -- Cultures of oligodendroblasts from primary cultures of rat neopallium / Bernhard H.J. Juurlink, Shawn K. Thorburne, and Richard M. Devon -- Olfactory ensheathing cell cultures / Ronald Doucette -- Cultures of stem cells of the central nervous system / Angela Gritti, Rossella Galli, and Angelo Luigi Vescovi -- Aggregating neural cell cultures / Paul Honegger and Florianne Monnet-Tschudi -- Neural cell lines / Kaoru Murayama [and others] -- Virus vectors for gene therapy of the nervous system / Sonia N. Yeung and Frank Tufaro -- Preparation of substrata for in vitro culture of neurons / Paul C. Letourneau -- Serum-fee media for neural cell cultures: adult and embryonic / Paul J. Price and Gregory J. Brewer.
Development of multiple-well plate biological assays / John w. Harbell -- Neural cell counting: cell number determination in microplate cultures / Marston Manthorpe -- Assays for neuronal cell migration / Toby N. Behar -- Colony culture: plating efficiency assay and cloning / Sergey Fedoroff and Arleen Richardson -- Hybridoma cultures for production of antibodies: purification and specificity of antibodies / J. Denry Sato -- Elimination of cell types from mixed neural cell cultures / Richard M. Devon -- Quantification of cells in culture / Arleen Richardson and Sergey Fedoroff -- Tips for tissue culture / Arleen Richardson and Sergey Fedoroff.
Action note: digitized 2010 committed to preserveSummary: In Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures. Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders.
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Includes bibliographical references and index.

In vitro assays for axonal growth and targeting / Valérie Castellani and Jürgen Bolz -- Interface organotypic hippocampal slice cultures / Dominique Muller [and others] -- Slice cultures for study of microglia / Carol A. Colton, Meggan Czapiga, and Toby N. Behar -- Microexplant cultures of the cerebellum / Bernard Rogister and Gustave Moonen -- Construction and use of compartmented cultures for studies of cell biology of neurons / Robert B. Campenot and Grace Martin -- Chick spinal somatic motoneurons in culture / Bernhard H.J. Juurlink -- Primary cultures of sympathetic ganglia / Mary I. Johnson -- Primary cell cultures for the study of myelination / Mary I. Johnson, Richard P. Bunge, and Patrick M. Wood -- Preparation of astrocyte, oligodendrocyte, and microglia cultures for primary rat cerebral cultures / Ruth Cole and Jean de Vellis -- Culture of glial cells from human brain biopsies / V. Wee Yong and Jack P. Antel.

Cultures of astroglia and microglia from primary cultures of mouse neopallium / Sergey Fedoroff and Arleen Richardson -- Cultures of oligodendroblasts from primary cultures of rat neopallium / Bernhard H.J. Juurlink, Shawn K. Thorburne, and Richard M. Devon -- Olfactory ensheathing cell cultures / Ronald Doucette -- Cultures of stem cells of the central nervous system / Angela Gritti, Rossella Galli, and Angelo Luigi Vescovi -- Aggregating neural cell cultures / Paul Honegger and Florianne Monnet-Tschudi -- Neural cell lines / Kaoru Murayama [and others] -- Virus vectors for gene therapy of the nervous system / Sonia N. Yeung and Frank Tufaro -- Preparation of substrata for in vitro culture of neurons / Paul C. Letourneau -- Serum-fee media for neural cell cultures: adult and embryonic / Paul J. Price and Gregory J. Brewer.

Development of multiple-well plate biological assays / John w. Harbell -- Neural cell counting: cell number determination in microplate cultures / Marston Manthorpe -- Assays for neuronal cell migration / Toby N. Behar -- Colony culture: plating efficiency assay and cloning / Sergey Fedoroff and Arleen Richardson -- Hybridoma cultures for production of antibodies: purification and specificity of antibodies / J. Denry Sato -- Elimination of cell types from mixed neural cell cultures / Richard M. Devon -- Quantification of cells in culture / Arleen Richardson and Sergey Fedoroff -- Tips for tissue culture / Arleen Richardson and Sergey Fedoroff.

In Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures. Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders.

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