Genomics protocols / edited by Michael P. Starkey and Ramnath Elaswarapu.

Contributor(s): Starkey, Michael P | Elaswarapu, Ramnath
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 175.Publisher: Totowa, N.J. : Humana Press, ©2001Description: 1 online resource (xiv, 538 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 0896037746; 9780896037748; 0585404372; 9780585404370; 159259235X; 9781592592357; 1280837314; 9781280837319; 9786610837311; 6610837317Subject(s): Genomics -- Laboratory manuals | Gene mapping -- Laboratory manuals | Chromosome Mapping | Molecular Biology | Sequence Analysis, DNA | Genomics | Biochemistry | Genetic Techniques | Computational Biology | Genetics | Chemistry | Biological Science Disciplines | Biology | Investigative Techniques | Natural Science Disciplines | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Disciplines and Occupations | Sequence Analysis | Génétique moléculaire -- Manuels de laboratoire | Cartes chromosomiques -- Manuels de laboratoire | SCIENCE -- Life Sciences -- Molecular Biology | Gene mapping | Genomics | Moleculaire geneticaGenre/Form: Laboratory Manuals | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Genomics protocols.DDC classification: 572.8 LOC classification: QH440.5 | G46 2001Other classification: 42.13 Online resources: Click here to access online
Contents:
1. Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse / Paul A. Lyons -- 2. Genetic Analysis of Complex Traits / Stephen P. Bryant and Mathias N. Chiano -- 3. Sequence-Based Detection of Single Nucleotide Polymorphisms / Deborah A. Nickerson, Natali Kolker, Scott L. Taylor and Mark J. Rieder -- 4. Genomic Mismatch Scanning for the Mapping of Genetic Traits / Farideh Mirzayans and Michael A. Walter -- 5. Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization / Mario A.J.A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer and Jan P.A. Baak -- 6. Construction of a Bacterial Artificial Chromosome Library / Sangdun Choi and Ung-Jin Kim -- 7. Contiguation of Bacterial Clones / Sean J. Humphray, Susan J. Knaggs and loannis Ragoussis -- 8. Mapping of Genomic Clones by Fluorescence In Situ Hybridization / Margaret A. Leversha -- 9. Map Integration : From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map / Panagiotis Deloukas -- 10. Construction of Full-Lengin-tnriched cDNA Libraries : The Oligo-Capping Method / Yutaka Suzuki and Sumio Sugano -- 11. Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping : The Human Gene Map / Panagiotis Deloukas -- 12. Preparation and Screening of High-Density cDNA Arrays with Genomic Clones / Gunther Zehetner, Maria Pack, and Katja Schafer -- 13. Direct Selection of cDNAs by Genomic Clones / Daniela Toniolo -- 14. Exon Trapping : Application of a Large-Insert Multiple-Exon-Trapping System / Martin C. Wapenaar and Johan T. Den Dunnen -- 15. Sequencing Bacterial Artificial Chromosomes / David E. Harris and Lee Murphy -- 16. Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics / Yvonne J.K. Edwards and Simon M. Brocklehurst -- 17. Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning / Greg Elgar -- 18. Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization / Oliver Dorian von Stein -- 19. Isolation of Differentially Expressed Genes by Representational Difference Analysis / Christine Wallrapp and Thomas M. Gress -- 20. Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display / Michael P. Starkey -- 21. Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos / Nicolas Pollet and Christof Niehrs -- 22. Expression Monitoring Using cDNA Microarrays : A General Protocol / Xing Jian Lou, Mark Schena, Frank T. Horrigan, Richard M. Lawn and Ronald W. Davis -- 23. Prediction of Protein Structure and Function by Using Bioinformatics / Yvonne J.K. Edwards and Amanda Cottage -- 24. Identification of Novel Genes by Gene Trap Mutagenesis / Anne K. Voss and Tim Thomas -- 25. Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice / Ahmed Mansouri -- 26. Gehomic Analysis Utilizing the Yeast Two-Hybrid System / llya G. Serebriiskii, Garabet G. Toby, Russell L. Finley, Jr. and Erica A. Golemis -- 27. Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle / Terry J. Amiss and Richard Jude Samulski -- 28. Retroviral-Mediated Gene Transduction / Donald S. Anson -- 29. Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods / Ruth Gjerset, All Haghighi, Svetlana Lebedeva, and Dan Mercola -- 30. Ribozyme Gene Therapy / Leonidas A. Phylactou.
Action note: digitized 2010 committed to preserveSummary: Genome analysis is essential both to understanding the molecular bases of physiological processes and to the development of novel therapies for treating human diseases. In Genomics Protocols, an expert panel of internationally acclaimed researchers from academia, industry, and leading genome centers provides a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. Highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function. Each method includes step-by-step instructions and invaluable notes that describe the quirks in a procedure and the little tricks that make all the difference to a successful outcome. Comprehensive and eminently practical, Genomics Protocols provides academic and pharmaceutical researchers alike with richly detailed accounts of the most up-to-date genomics techniques, including the emerging technologies from proteomics-all highly effective tools that will help investigators expand their research horizons and better understand gene function.
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Includes bibliographical references and index.

1. Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse / Paul A. Lyons -- 2. Genetic Analysis of Complex Traits / Stephen P. Bryant and Mathias N. Chiano -- 3. Sequence-Based Detection of Single Nucleotide Polymorphisms / Deborah A. Nickerson, Natali Kolker, Scott L. Taylor and Mark J. Rieder -- 4. Genomic Mismatch Scanning for the Mapping of Genetic Traits / Farideh Mirzayans and Michael A. Walter -- 5. Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization / Mario A.J.A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer and Jan P.A. Baak -- 6. Construction of a Bacterial Artificial Chromosome Library / Sangdun Choi and Ung-Jin Kim -- 7. Contiguation of Bacterial Clones / Sean J. Humphray, Susan J. Knaggs and loannis Ragoussis -- 8. Mapping of Genomic Clones by Fluorescence In Situ Hybridization / Margaret A. Leversha -- 9. Map Integration : From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map / Panagiotis Deloukas -- 10. Construction of Full-Lengin-tnriched cDNA Libraries : The Oligo-Capping Method / Yutaka Suzuki and Sumio Sugano -- 11. Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping : The Human Gene Map / Panagiotis Deloukas -- 12. Preparation and Screening of High-Density cDNA Arrays with Genomic Clones / Gunther Zehetner, Maria Pack, and Katja Schafer -- 13. Direct Selection of cDNAs by Genomic Clones / Daniela Toniolo -- 14. Exon Trapping : Application of a Large-Insert Multiple-Exon-Trapping System / Martin C. Wapenaar and Johan T. Den Dunnen -- 15. Sequencing Bacterial Artificial Chromosomes / David E. Harris and Lee Murphy -- 16. Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics / Yvonne J.K. Edwards and Simon M. Brocklehurst -- 17. Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning / Greg Elgar -- 18. Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization / Oliver Dorian von Stein -- 19. Isolation of Differentially Expressed Genes by Representational Difference Analysis / Christine Wallrapp and Thomas M. Gress -- 20. Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display / Michael P. Starkey -- 21. Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos / Nicolas Pollet and Christof Niehrs -- 22. Expression Monitoring Using cDNA Microarrays : A General Protocol / Xing Jian Lou, Mark Schena, Frank T. Horrigan, Richard M. Lawn and Ronald W. Davis -- 23. Prediction of Protein Structure and Function by Using Bioinformatics / Yvonne J.K. Edwards and Amanda Cottage -- 24. Identification of Novel Genes by Gene Trap Mutagenesis / Anne K. Voss and Tim Thomas -- 25. Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice / Ahmed Mansouri -- 26. Gehomic Analysis Utilizing the Yeast Two-Hybrid System / llya G. Serebriiskii, Garabet G. Toby, Russell L. Finley, Jr. and Erica A. Golemis -- 27. Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle / Terry J. Amiss and Richard Jude Samulski -- 28. Retroviral-Mediated Gene Transduction / Donald S. Anson -- 29. Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods / Ruth Gjerset, All Haghighi, Svetlana Lebedeva, and Dan Mercola -- 30. Ribozyme Gene Therapy / Leonidas A. Phylactou.

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Genome analysis is essential both to understanding the molecular bases of physiological processes and to the development of novel therapies for treating human diseases. In Genomics Protocols, an expert panel of internationally acclaimed researchers from academia, industry, and leading genome centers provides a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. Highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function. Each method includes step-by-step instructions and invaluable notes that describe the quirks in a procedure and the little tricks that make all the difference to a successful outcome. Comprehensive and eminently practical, Genomics Protocols provides academic and pharmaceutical researchers alike with richly detailed accounts of the most up-to-date genomics techniques, including the emerging technologies from proteomics-all highly effective tools that will help investigators expand their research horizons and better understand gene function.

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