Two-hybrid systems : methods and protocols / edited by Paul N. MacDonald.
Contributor(s): MacDonald, Paul NMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 177.Publisher: Totowa, N.J. : Humana Press, ©2001Description: 1 online resource (xii, 336 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592592104; 0585403244; 9780585403243; 1592592104Subject(s): Protein binding -- Research -- Methodology | Yeast fungi | Plasmids | Carrier Proteins | Yeasts | Plasmids | Laboratory Techniques and Procedures | Protéines -- Fixation -- Recherche -- Méthodologie | Levures (Botanique) | Plasmides | SCIENCE -- Life Sciences -- Biochemistry | Plasmids | Yeast fungi | Eiwitten | Gist | Plasmiden | Wetenschappelijke technieken | Biowissenschaften | Proteinbindung | Labortechnik | Yeast-Two-Hybrid-System | Aufsatzsammlung | Plasmid ; SWD-ID: 40462705 | Plasmids | Protein binding Research Methodology | Saccharomycetaceae ; SWD-ID: 41316149 | Two-Hybrid System Techniques | Yeast fungi | Yeasts / geneticsGenre/Form: Electronic books. Additional physical formats: Print version:: Two-hybrid systems.DDC classification: 572/.6 LOC classification: QP551 | .T865 2001Other classification: 35.75 | 42.13 | 44.33 Online resources: Click here to access online
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Includes bibliographical references and index.
Part I. General Methods -- The Two-Hybrid System : A Personal View / Stanley Fields and Paul L. Bartel -- Growth and Maintenance of Yeast / Lawrence W. Bergman -- Media Formulations for Various Two-Hybrid Systems / Michael Saghbini, Denise Hoekstra, and Jim Gautsch -- Yeast Two-Hybrid Vectors and Strains / Philip James -- High-Efficiency Transformation of Plasmid DNA into Yeast / Robin A. Woods and R. Daniel Gietz -- Qualitative and Quantitative Assessment of Interactions / Monica M. Montano -- Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies / Alyson Byrd and René St-Arnaud -- Part II. False Positives -- Two-Hybrid System and False Positives : Approaches to Detection and Elimination / Ilya G. Serebriiskii and Erica A. Golemis -- Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns / Dennis M. Kraichely and Paul N. MacDonald -- Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones / Louie Naumovski -- Part III. Applications -- Smad Interactors in Bone Morphogenetic Protein Signaling / Xiangli Yang and Xu Cao -- Protein Interactions Important in Eukaryotic Translation Initiation / Katsura Asano and Alan G. Hinnebusch -- Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins / Sergio A. Oñate -- Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-apoptosis Protein EB-K / Thirugnana Subramanian and G. Chinnadurai -- Part IV. Alternative Strategies -- Mammalian Two-Hybrid Assays : Analyzing Protein-Protein Interactions in the Transforming Growth Factor-[beta] Signaling Pathway / Xin-Hua Feng and Rik Derynck -- One-Hybrid Systems for Detecting Protein-DNA Interactions / Mary Kate Alexander, Brenda D. Bourns, and Virginia A. Zakian -- The Split-Hybrid System : Uncoding Multiprotein Networks and Defining Mutations That Affect Protein Interactions / Phyllis S. Goldman [and others] -- Three-Hybrid Screens : Inducible Third-Party Systems / Björn Sandrock, Franck Tirode, and Jean-Marc Egly -- Three-Hybrid Screens for RNA-Binding Proteins : Proteins Binding 3' End of Histone mRNA / Zbigniew Dominski and William F. Marzluff -- Membrane Recruitment Systems for Analysis of Protein-Protein Interactions / Ami Aronheim.
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The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast) to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problems of artifacts and false positives and for identifying the interacting partners in important biological systems, including the Smad and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly. Comprehensive and highly practical, Two-Hybrid Systems: Methods and Protocols not only reveals how the great variety of plasmid vectors and approaches may be optimally deployed, but also quickly empowers novices to establish two-hybrid systems in their laboratories, and experienced researchers to expand their repertoire of techniques.