Neural stem cells : methods and protocols / edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos.Material type: TextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 198.Publication details: Totowa, N.J. : Humana Press, ©2002. Description: 1 online resource (xiv, 381 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 0585403252; 9780585403250Subject(s): Neurons -- Laboratory manuals | Stem cells -- Laboratory manuals | Neurons -- cytology | Stem Cells -- cytology | Stem Cells -- physiology | Cytological Techniques -- methods | SOCIAL SCIENCE -- Anthropology -- Physical | Neurons | Stem cells | neuronen | neurons | zenuwweefsel | nerve tissue | cellen | cells | celbiologie | cellular biology | biologische technieken | biological techniques | neurobiologie | neurobiology | stamcellen | stem cells | protocollen | protocols | Cellular Biology | CelbiologieGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Neural stem cells.DDC classification: 573.8/536 LOC classification: QP357 | .N473 2002ebNLM classification: W1 | QP 357Online resources: Click here to access online
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Includes bibliographical references and index.
Part I. Isolation and Culture of NSCs -- 1. Neural Differentiation of Embryonic Stem Cells / K. Sue O'Shea -- 2. Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens / Eric D. Laywell [and others] -- 3. Isolation of Stem and Precursor Cells from Fetal Tissue / Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao -- 4. Olfactory Ensheathing Cells : Isolation and Culture from the Rat Olfactory Bulb / Susan C. Barnett and A. Jane Roskams -- 5. Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium / Edmund Au and A. Jane Roskams -- 6. Production of Immortalized Human Neural Crest Stem Cells / Seung U. Kim [and others] -- 7. Adult Rodent Spinal Cord Derived Neural Stem Cells : Isolation and Characterization / Lamya S. Shihabuddin -- 8. Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood / Shijie Song and J. Sanchez-Ramos -- 9. Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers / Erin Lavik [and others] -- Part II. Characterization of NSCs In Vitro -- A. Cellular Techniques -- 10. Analysis of Cell Generation in the Telencephalic Neuroepithelium / Takao Takahashi, Verne S. Caviness, Jr., and Pradeep G. Bhide -- 11. Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures / Angelo L. Vescovi, Rossella Galli, and Angela Gritti -- 12. Assessing the Involvement of Telomerase in Stem Cell Biology / Mark P. Mattson, Peisu Zhang, and Weiming Fu -- 13. Detection of Telomerase Activity in Neural Cells / Karen R. Prowse -- 14. In Vitro Assays for Neural Stem Cell Differentiation / Marcel M. Daadi -- 15. Electron Microscopy and Lac-Z Labeling / Bela Kosaras and Evan Snyder -- B. Electrophysiological Techniques -- 16. Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells / David S.K. Magnuson and Dante J. Morassutti -- C. Molecular Techniques -- 17. Fluorescence In Situ Hybridization / Barbara A. Tate and Rachel L. Ostroff -- 18. RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells / Theresa E. Gratsch -- 19. Differential Display : Isolation of Novel Genes / Theresa E. Gratsch -- 20. Cell Labeling and Gene Misexpression by Electroporation / Terence J. Van Raay and Michael R. Stark -- 21. Gene Therapy Using Neural Stem Cells / Luciano Conti and Elena Cattaneo -- 22. Modeling Brain Pathologies Using Neural Stem Cells / Simonetta Siplone and Elena Cattaneo -- Part III. Utilization/Characterization of NSCs In Vivo -- A. Endogenous Pools of Stem/Progenitor Cells -- 23. Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model / Marcel M. Daadi -- 24. Identification of Musashil-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods / Yonehiro Kanemura, Shin-ichi Sakakibara, and Hideyuki Okano -- 25. Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo -- Sanjay S.P. Magavi and Jeffrey D. Macklis283 -- 26. Immunocytochemical Analysis of Neuronal Differentiation / Sanjay S.P. Magavi and Jeffrey D. Macklis -- 27. Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold / Lisa A. Catapano, Sanjay S.P. Magavi, and Jeffrey D. Macklis -- B. Transplantation -- 28. Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS / Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore -- 29. Optimizing Stem Cell Grafting into the CNS / Scott R. Whittemore [and others] -- 30. Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos / Lorenzo Magrassi -- 31. Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells / Tanja Zigova and Mary B. Newman -- 32. Routes of Stem Cell Administration in the Adult Rodent / Alison E. Willing [and others].
Print version record.
Annotation In Neural Stem Cells: Methods and Protocols, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.