Calcium-binding protein protocols. Volume 2, Methods and techniques / edited by Hans J. Vogel.

Contributor(s): Vogel, Hans J
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): . v. 173.Publisher: Totowa, N.J. : Humana Press, ©2002Description: 1 online resource (415 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781592591848; 0585419728; 9780585419725; 1592591841Other title: Calcium binding protein protocols | Methods and techniquesSubject(s): Calcium-binding proteins -- Research -- Methodology | Calcium-Binding Proteins | Research Design | SCIENCE -- Life Sciences -- BiochemistryGenre/Form: Electronic books. Additional physical formats: Print version:: Calcium-binding protein protocols. Volume 2, Methods and techniques.DDC classification: 572/.69 LOC classification: QP552.C24 | C33 2002ebOnline resources: Click here to access online
Contents:
Calcium-Binding Protein Protocols -- Dedication -- Preface -- Contents -- CONTENTS OF THE COMPANION VOLUME -- Contributors -- METHODS AND TECHNIQUES TO STUDY CALCIUM-BINDING PROTEINS -- Quantitative Analysis of Ca2+-Binding by Flow Dialysis -- Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators -- Deconvolution of Calcium-Binding Curves Facts and Fantasies -- Absorption and Circular Dichroism Spectroscopy -- Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins -- Steady-State Fluorescence Spectroscopy.
Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins -- Surface Plasmon Resonance of Calcium-Binding Proteins -- Differential Scanning Calorimetry -- Isothermal Titration Calorimetry -- Multiangle Laser Light Scattering and Sedimentation Equilibrium -- Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in their Interactions with Regulatory Targets -- Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry -- Synthetic Calcium-Binding Peptides.
Proteolytic Fragments of Calcium-Binding Proteins -- Electron Magnetic Resonance Studies of Calcium-Binding Proteins -- Cadmium-113 and Lead-207 NMR Spectroscopic Studies of Calcium-Binding Proteins -- Calcium-43 NMR of Calcium-Binding Proteins -- Exploring Familial Relationships Using Multiple Sequence Alignment -- Structure Determination by NMR Isotope Labeling -- Protein Structure Calculation from NMR Data -- Shape and Dynamics of a Calcium-Binding Protein Investigated by Nitrogen-15 NMR Relaxation.
The Use of Dipolar Couplings for the Structure Refinement of a Pair of Calcium-Binding EGF Domains -- Vector Geometry Mapping A Method to Characterize the Conformation of Helix-Loop-Helix Calcium-Binding Proteins -- Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography -- Enzymatic Assays to Compare Calmodulin Isoforms, Mutants, and Chimeras -- Gene Expression in Transfected Cells -- Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins.
Studying the Spatial Distribution of Ca2+-Binding Proteins How Does it Work for Calmodulin? -- Index.
Summary: Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols- Volume 1: Reviews and Case Histories and Volume 2: Methods and Techniques-Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant class proteins, and provide a comprehensive collection of proven experimental techniques for studying it both in vitro and in vivo. This second volume focuses on cutting-edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells. The first companion volume, Reviews and Case Histories sets the stage for this volume by introducing the various classes of intra- and extracellular calcium-binding proteins and their mode of action. Comprehensive and highly practical, the two volumes of Calcium-Binding Protocols provide experimental and clinical biologists with a host of advanced experimental methods that can be applied successfully to the study of both existing and newly discovered members of this critically important class of proteins.
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Includes bibliographical references and index.

Print version record.

Calcium-Binding Protein Protocols -- Dedication -- Preface -- Contents -- CONTENTS OF THE COMPANION VOLUME -- Contributors -- METHODS AND TECHNIQUES TO STUDY CALCIUM-BINDING PROTEINS -- Quantitative Analysis of Ca2+-Binding by Flow Dialysis -- Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators -- Deconvolution of Calcium-Binding Curves Facts and Fantasies -- Absorption and Circular Dichroism Spectroscopy -- Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins -- Steady-State Fluorescence Spectroscopy.

Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins -- Surface Plasmon Resonance of Calcium-Binding Proteins -- Differential Scanning Calorimetry -- Isothermal Titration Calorimetry -- Multiangle Laser Light Scattering and Sedimentation Equilibrium -- Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in their Interactions with Regulatory Targets -- Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry -- Synthetic Calcium-Binding Peptides.

Proteolytic Fragments of Calcium-Binding Proteins -- Electron Magnetic Resonance Studies of Calcium-Binding Proteins -- Cadmium-113 and Lead-207 NMR Spectroscopic Studies of Calcium-Binding Proteins -- Calcium-43 NMR of Calcium-Binding Proteins -- Exploring Familial Relationships Using Multiple Sequence Alignment -- Structure Determination by NMR Isotope Labeling -- Protein Structure Calculation from NMR Data -- Shape and Dynamics of a Calcium-Binding Protein Investigated by Nitrogen-15 NMR Relaxation.

The Use of Dipolar Couplings for the Structure Refinement of a Pair of Calcium-Binding EGF Domains -- Vector Geometry Mapping A Method to Characterize the Conformation of Helix-Loop-Helix Calcium-Binding Proteins -- Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography -- Enzymatic Assays to Compare Calmodulin Isoforms, Mutants, and Chimeras -- Gene Expression in Transfected Cells -- Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins.

Studying the Spatial Distribution of Ca2+-Binding Proteins How Does it Work for Calmodulin? -- Index.

Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols- Volume 1: Reviews and Case Histories and Volume 2: Methods and Techniques-Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant class proteins, and provide a comprehensive collection of proven experimental techniques for studying it both in vitro and in vivo. This second volume focuses on cutting-edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells. The first companion volume, Reviews and Case Histories sets the stage for this volume by introducing the various classes of intra- and extracellular calcium-binding proteins and their mode of action. Comprehensive and highly practical, the two volumes of Calcium-Binding Protocols provide experimental and clinical biologists with a host of advanced experimental methods that can be applied successfully to the study of both existing and newly discovered members of this critically important class of proteins.

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