Molecular analysis of cancer / edited by Jacqueline Boultwood and Carrie Fidler.

Contributor(s): Boultwood, Jacqueline | Fidler, Carrie
Material type: TextTextSeries: Methods in molecular medicine: 68.Publisher: Totowa, N.J. : Humana Press, ©2002Description: 1 online resource (xi, 302 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781592591350; 1592591353; 0585438919; 9780585438917; 9786610821020; 661082102X; 1280821027; 9781280821028Subject(s): Cancer -- Genetic aspects -- Research -- Methodology | Cancer -- Molecular aspects -- Research -- Methodology | Neoplasms -- genetics | Cell Transformation, Neoplastic -- genetics | Gene Expression Regulation, Neoplastic | Genetic Techniques | MEDICAL -- Oncology | HEALTH & FITNESS -- Diseases -- Cancer | Medicine | Pathology | Krebs Medizin | Molekulare Medizin | Kanker | Moleculaire biologie | Wetenschappelijke techniekenGenre/Form: Electronic books. Additional physical formats: Print version:: Molecular analysis of cancer.DDC classification: 616.99/4042 LOC classification: RC268.4 | .M627 2002Other classification: 44.81 | XH 2611 Online resources: Click here to access online
Contents:
Molecular analysis of cancer. An overview -- Detection of chromosome abnormalities in leukemia using fluorescence in situ hybridization -- Spectral karyotyping in cancer cytogenetics -- Comparative genomic hybridization analysis -- Detection of chromosomal deletions by microsatellite analysis -- Detection and quantification of leukemia-specific rearrangements -- Detection of t(2;5)(p23;q35) translocation by long-range PCR of genomic DNA -- Use of DNA fingerprinting to detect genetic rearrangements in human cancer -- Mutation analysis of large genomic regions in tumor DNA using single-strand conformation polymorphism. Lessons from the ATM gene -- Mutational analysis of oncogenes and tumor suppressor genes in human cancer using denaturing gradient gel electrophoresis -- Detection of mutations in human cancer using nonisotopic RNase cleavage assay -- Mutational analysis of the neurofibromatosis type 1 gene in childhood myelodysplastic syndromes using a protein truncation assay -- Mutation analysis of cancer using automated sequencing -- Detection of differentially expressed genes in cancer using differential display -- Genomewide gene expression analysis using cDNA microarrays -- Gene expression profiling in cancer using cDNA microarrays -- Wilms tumor gene WT1 as a tumor marker for leukemic blast cells and its role in leukemogenesis -- Detection of aberrant methylation of the p15INK4B gene promoter -- Clonality studies in cancer based on X chromosome inactivation phenomenon -- Telomere length changes in human cancer -- Measurement of telomerase activity in human hematopoietic cells and neoplastic disorders.
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: Molecular biotechnology has in recent years produced significant advances in finding tumor markers useful in diagnosis and in the assessment of disease progression and prognosis. In Molecular Analysis of Cancer, leading researchers in the field describe in step-by-step detail their best state-of-the-art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations. Techniques for the analysis of gene expression include microarray technology and differential display. All these techniques are of great value for identifying genes that are central to cancer development and progression. Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities.
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Includes bibliographical references and index.

Molecular analysis of cancer. An overview -- Detection of chromosome abnormalities in leukemia using fluorescence in situ hybridization -- Spectral karyotyping in cancer cytogenetics -- Comparative genomic hybridization analysis -- Detection of chromosomal deletions by microsatellite analysis -- Detection and quantification of leukemia-specific rearrangements -- Detection of t(2;5)(p23;q35) translocation by long-range PCR of genomic DNA -- Use of DNA fingerprinting to detect genetic rearrangements in human cancer -- Mutation analysis of large genomic regions in tumor DNA using single-strand conformation polymorphism. Lessons from the ATM gene -- Mutational analysis of oncogenes and tumor suppressor genes in human cancer using denaturing gradient gel electrophoresis -- Detection of mutations in human cancer using nonisotopic RNase cleavage assay -- Mutational analysis of the neurofibromatosis type 1 gene in childhood myelodysplastic syndromes using a protein truncation assay -- Mutation analysis of cancer using automated sequencing -- Detection of differentially expressed genes in cancer using differential display -- Genomewide gene expression analysis using cDNA microarrays -- Gene expression profiling in cancer using cDNA microarrays -- Wilms tumor gene WT1 as a tumor marker for leukemic blast cells and its role in leukemogenesis -- Detection of aberrant methylation of the p15INK4B gene promoter -- Clonality studies in cancer based on X chromosome inactivation phenomenon -- Telomere length changes in human cancer -- Measurement of telomerase activity in human hematopoietic cells and neoplastic disorders.

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Molecular biotechnology has in recent years produced significant advances in finding tumor markers useful in diagnosis and in the assessment of disease progression and prognosis. In Molecular Analysis of Cancer, leading researchers in the field describe in step-by-step detail their best state-of-the-art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations. Techniques for the analysis of gene expression include microarray technology and differential display. All these techniques are of great value for identifying genes that are central to cancer development and progression. Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities.

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