Atherosclerosis : experimental methods and protocols / edited by Angela F. Drew.

Contributor(s): Drew, Angela F
Material type: TextTextSeries: Methods in molecular medicine: 52.Publisher: Totowa, N.J. : Humana Press, ©2001Description: 1 online resource (xi, 247 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592590735; 159259073X; 0896037517; 9780896037519; 1280821655; 9781280821653; 9786610821655; 6610821658Subject(s): Atherosclerosis -- Laboratory manuals | Atherosclerosis | Clinical Protocols | Research Design | MEDICAL -- Cardiology | Atherosclerosis | Wetenschappelijke technieken | Arteriosklerose | Molekulare MedizinGenre/Form: Electronic books. | Handbooks and manuals. Additional physical formats: AtherosclerosisDDC classification: 616.1/36/072 LOC classification: RC692 | .A7288 2001Other classification: YC 1111 Online resources: Click here to access online
Contents:
Animal models of diet-induced atherosclerosis / A.F. Drew -- Mechanical injury models: balloon catheter injury to rat common carotid artery / R.J. Dilley -- Genetically manipulated models of atherosclerosis in mice / Q. Xiao -- Lipoprotein isolation and analysis from serum by preparative ultracentrifugation / K.M. Wasan -- Separation of plasma lipoproteins in self-generated gradients of iodixanol / J.A. Higgins -- Fractionation of lipoprotein subclasses in self-generated gradients of iodixanol / J.M. Graham -- Detecting cholesteryl ester transfer protein in plasma / A. Ritsch -- Determination of plasma homocysteine / R. Accinni -- Assay for serum glycated lipoproteins / A. Tanaka -- Lipoprotein (a) quantitation / M. Bender -- Isolation and characterization of glycosaminoglycans from human atheromatous vessels / E. Papakonstantinou -- Culture of human smooth muscle cells / M.A. Gallicchio -- Culture of human endothelial cells / M.A. Gallicchio -- Assay for cell migration and invasion of vascular smooth muscle cells / G. Karakiulakis -- Collection and processing of arterial specimens for histological analysis / A.F. Drew -- Intravascular ultrasound imaging in the quantitation of atherosclerosis in vivo / H.I. Manninen -- Identification of cell types and quantification of lesion composition / R.J. Dilley -- Nonradioactive in situ hybridization in atherosclerosis tissue / J. Apostolopoulos -- Evaluation of angiogenesis / M. Silver -- Evaluation of cell proliferation in human atherosclerotic lesions / E.R. Andreeva -- Gene transfer to the vasculature: historical perspective and implications for future research objectives / S.J. George -- A pig model of vein graft disease: applications for potential gene therapies / C.T. Lloyd.
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: Atherosclerosis-a major killer of people in Western societies-is not yet completely understood. In Atherosclerosis: Methods and Protocols, Angela Drew and a panel of experts have assembled a comprehensive collection of traditional and cutting-edge techniques for investigating this disease and its possible treatments. Each readily reproducible method includes step-by-step instructions and practical information regarding sample collection, the choice of animal model system, experimental design, and practical data analysis techniques. Assay systems for serum or plasma determination of lipoproteins are also described, along with assays for those plasma proteins recently identified as implicated in atherogenesis, including cholestryl ester transfer protein, homocysteine, glycated lipoproteins, and apolipoprotein(a). Additional techniques cover the isolation and culture of cells and glycosaminoglycans from atherosclerotic plaques, and the collection and analysis of experimental atherosclerotic lesions. Comprehensive and richly detailed, Atherosclerosis: Methods and Protocols enables all biomedical investigators to select those optimized techniques that may be most fruitfully used to study the development, progression, and treatment of atherosclerotic lesions today.
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Animal models of diet-induced atherosclerosis / A.F. Drew -- Mechanical injury models: balloon catheter injury to rat common carotid artery / R.J. Dilley -- Genetically manipulated models of atherosclerosis in mice / Q. Xiao -- Lipoprotein isolation and analysis from serum by preparative ultracentrifugation / K.M. Wasan -- Separation of plasma lipoproteins in self-generated gradients of iodixanol / J.A. Higgins -- Fractionation of lipoprotein subclasses in self-generated gradients of iodixanol / J.M. Graham -- Detecting cholesteryl ester transfer protein in plasma / A. Ritsch -- Determination of plasma homocysteine / R. Accinni -- Assay for serum glycated lipoproteins / A. Tanaka -- Lipoprotein (a) quantitation / M. Bender -- Isolation and characterization of glycosaminoglycans from human atheromatous vessels / E. Papakonstantinou -- Culture of human smooth muscle cells / M.A. Gallicchio -- Culture of human endothelial cells / M.A. Gallicchio -- Assay for cell migration and invasion of vascular smooth muscle cells / G. Karakiulakis -- Collection and processing of arterial specimens for histological analysis / A.F. Drew -- Intravascular ultrasound imaging in the quantitation of atherosclerosis in vivo / H.I. Manninen -- Identification of cell types and quantification of lesion composition / R.J. Dilley -- Nonradioactive in situ hybridization in atherosclerosis tissue / J. Apostolopoulos -- Evaluation of angiogenesis / M. Silver -- Evaluation of cell proliferation in human atherosclerotic lesions / E.R. Andreeva -- Gene transfer to the vasculature: historical perspective and implications for future research objectives / S.J. George -- A pig model of vein graft disease: applications for potential gene therapies / C.T. Lloyd.

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Atherosclerosis-a major killer of people in Western societies-is not yet completely understood. In Atherosclerosis: Methods and Protocols, Angela Drew and a panel of experts have assembled a comprehensive collection of traditional and cutting-edge techniques for investigating this disease and its possible treatments. Each readily reproducible method includes step-by-step instructions and practical information regarding sample collection, the choice of animal model system, experimental design, and practical data analysis techniques. Assay systems for serum or plasma determination of lipoproteins are also described, along with assays for those plasma proteins recently identified as implicated in atherogenesis, including cholestryl ester transfer protein, homocysteine, glycated lipoproteins, and apolipoprotein(a). Additional techniques cover the isolation and culture of cells and glycosaminoglycans from atherosclerotic plaques, and the collection and analysis of experimental atherosclerotic lesions. Comprehensive and richly detailed, Atherosclerosis: Methods and Protocols enables all biomedical investigators to select those optimized techniques that may be most fruitfully used to study the development, progression, and treatment of atherosclerotic lesions today.

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