Vaccine protocols / edited by Andrew Robinson, Michael J. Hudson, and Martin P. Cranage.
Contributor(s): Robinson, Andrew | Hudson, Michael J | Cranage, Martin PMaterial type: TextSeries: Methods in molecular medicine: 87.Publisher: Totowa, N.J. : Humana Press, ©2003Edition: 2nd edDescription: 1 online resource (xi, 414 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592593996; 1588291405; 9781588291400; 1592593992Subject(s): Vaccines -- Laboratory manuals | Vaccines | Drug Design | Genetic Techniques | Research Design | MEDICAL -- Pharmacology | VaccinesGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Vaccine protocols.DDC classification: 615/.372 LOC classification: QR189 | .V2513 2003Online resources: Click here to access online
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Includes bibliographical references and index.
Overview of vaccines / G. Ada -- Temperature-sensitive mutant vaccines / C.R. Pringle -- Live viral vectors: construction of a replication-deficient recombinant adenovirus / A.R. Fooks -- Live viral vectors: vaccinia virus / C. Staib and G. Sutter -- Live viral vectors: Semliki Forest virus / G.B. Karlsson and P. Liljestrom -- Development of attenuated Salmonella strains that express heterologous antigens / F. Bowe [and others] -- Expression and delivery of heterologous antigens using lactic acid bacteria / M.A. Reuter, S. Hanniffy and J.M. Wells -- Synthetic peptides / M.J. Francis -- Genetic detoxification of bacterial toxins / M. Pizza [and others] -- Preparation of polysaccharide-conjugate vaccines / C.C. Peeters [and others] -- Adjuvant formulations for experimental vaccines / D.E. Stewart-Tull -- Incorporation of immunomodulators into plasmid DNA vaccines / L.S. Klavinskis, P. Hobson and A. Woods -- Microencapsulation of vaccine antigens / D.H. Jones -- Lyophilization of vaccines: current trends / G.D. Adams -- Stimulation of mucosal immunity / D.J. Lewis and C.M. Hayward -- Induction and detection of T-cell responses / K.H. Mills -- Construction of MHC class I-peptide tetrameric complexes for analysis of T-cell-mediated immune responses / R.V. Samuel and T. Hanke -- Assessment of functional antibody responses / R. Borrow and P. Balmer -- The use of complete genome sequences in vaccine design / N.J. Saunders and S. Butcher -- Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment / M.J. Dennis -- Clinical trials / P. Farrington and E. Miller -- Assuring the quality and safety of vaccines: regulatory licensing and batch release / E. Griffiths and I. Knezevic -- DNA vaccination: an update / D.B. Lowrie -- From vaccine research to manufacture: a guide for the researcher / N. Allison and H.S. Tranter.
Print version record.
This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines through the genetic manipulation of viruses and bacteria, vaccine vectors or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. In addition, there are chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials. New to this second edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. State-of-the-art and highly practical, Vaccine Protocols, Second Edition, provides essential information for those seeking to produce improved or novel vaccines in the rapidly evolving field of vaccine research and development.