Antibacterial peptide protocols / edited by William M. Shafer.

Contributor(s): Shafer, William M
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 78.Publisher: Totowa, N.J. : Humana Press, ©1997Description: 1 online resource (x, 259 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592595648; 1592595642; 1280836830; 9781280836831; 9786610836833; 6610836833Subject(s): Peptide antibiotics -- Research -- Methodology | Peptide antibiotics -- Analysis | Anti-Bacterial Agents -- analysis | Peptides -- analysis | Genetic Techniques | Microbiological Techniques | Peptide antibiotics -- Analysis | Peptiden | Antibacteriële middelen | Infectieziekten | Wetenschappelijke technieken | moleculaire biologie | molecular biology | micro-organismen | microorganisms | bacteria | classificatie | classification | taxonomie | taxonomy | Proteins and Enzymes | Eiwitten en enzymenGenre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Antibacterial peptide protocols.DDC classification: 615/.329 LOC classification: RS431.P37 | A57 1997Other classification: 44.38 Online resources: Click here to access online
Contents:
Origins and development of peptide antibiotic research : from extracts to abstracts to contracts / John K. Spitznagel -- HPLC methods for purification of antimicrobial peptides / Michael E. Selsted -- Strategies for the isolation and characterization of antimicrobial peptides of invertebrates / Charles Hetru, Philippe Bulet -- Big defensin and tachylectins-1 and -2 / Shun-ichiro Kawabata, Sadaaki Iwanaga -- Tachyplesin and anti-lipopolysaccharide factor / Tatsushi Muta, Sadaaki Iwanaga -- Circular dichroism studies of secondary structure of peptides / Martha M. Juban, Maryam M. Javadpour, Mary D. Barkley -- Analytical ultracentrifugation studies of association of peptides / Martha M. Juban, Maryam M. Javadpour, Mary D. Barkley -- NMR characterization of amphipathic helical peptides / Xiaotang Wang, Kathleen M. Morden -- Laboratory production of antimicrobial peptides in native conformation / Erika V. Valore, Tomas Ganz -- An approach combining rapid cDNA amplification and chemical synthesis or the identification of novel, cathelicidin-derived, antimicrobial peptides / Alessandro Tossi, Marco Scocchi, Margherita Zanetti, Renato Gennaro, Paola Storici, Domenico Romeo -- Molecular biological strategies in the analysis of antibiotic peptide gene families : the use oligonucleotides as hybridization probes / Charles L. Bevins, Gill Diamond -- Designer assays for antimicrobial peptides: disputing the "one-size-fits-all" theory / Deborah A. Steinberg, Robert I. Lehrer -- Interaction of cationic peptides with bacterial membranes / Shafique Fidai, Susan W. Farmer, Robert E. Hancock -- The genetic basis of microbial resistance to antimicrobial peptides / Eduardo A. Groisman, Arden Aspedon -- Assay of antibacterial activities of the bactericidal/permeability-increasing protein in natural biological fluids / Jerrold Weiss -- Assay systems for measurement of chemotactic activity / H. Anne Pereira -- Neutralization of the in vivo activity of E. coli-derived lipopolysaccharide by cationic peptides / Daniel J. Brackett, Megan R. Lerner, H. Anne Pereira.
Action note: digitized 2010 committed to preserveSummary: In Antibacterial Peptide Protocols, leading authorities review for the first time in one volume all the major biochemical, molecular, bacteriological, and physical techniques available to assess antimicrobial peptides. These state-of-the-art methods ensure easily reproducible results in such important procedures as the isolation and characterization of antimicrobial peptides, the molecular characterization of genes encoding antimicrobial peptides, and the use of expression systems to isolate peptides. Bioassays and microbial genetic techniques are also included, as are antibacterial assays as the final readout system. These methods detailed in Antibacterial Peptide Protocols will play an important role in the treatment of infectious diseases, particularly with the increasing problem of multidrug-resistant microbes and the relative dearth of new antibiotics being provided by pharmaceutical companies.
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Includes bibliographical references and index.

Origins and development of peptide antibiotic research : from extracts to abstracts to contracts / John K. Spitznagel -- HPLC methods for purification of antimicrobial peptides / Michael E. Selsted -- Strategies for the isolation and characterization of antimicrobial peptides of invertebrates / Charles Hetru, Philippe Bulet -- Big defensin and tachylectins-1 and -2 / Shun-ichiro Kawabata, Sadaaki Iwanaga -- Tachyplesin and anti-lipopolysaccharide factor / Tatsushi Muta, Sadaaki Iwanaga -- Circular dichroism studies of secondary structure of peptides / Martha M. Juban, Maryam M. Javadpour, Mary D. Barkley -- Analytical ultracentrifugation studies of association of peptides / Martha M. Juban, Maryam M. Javadpour, Mary D. Barkley -- NMR characterization of amphipathic helical peptides / Xiaotang Wang, Kathleen M. Morden -- Laboratory production of antimicrobial peptides in native conformation / Erika V. Valore, Tomas Ganz -- An approach combining rapid cDNA amplification and chemical synthesis or the identification of novel, cathelicidin-derived, antimicrobial peptides / Alessandro Tossi, Marco Scocchi, Margherita Zanetti, Renato Gennaro, Paola Storici, Domenico Romeo -- Molecular biological strategies in the analysis of antibiotic peptide gene families : the use oligonucleotides as hybridization probes / Charles L. Bevins, Gill Diamond -- Designer assays for antimicrobial peptides: disputing the "one-size-fits-all" theory / Deborah A. Steinberg, Robert I. Lehrer -- Interaction of cationic peptides with bacterial membranes / Shafique Fidai, Susan W. Farmer, Robert E. Hancock -- The genetic basis of microbial resistance to antimicrobial peptides / Eduardo A. Groisman, Arden Aspedon -- Assay of antibacterial activities of the bactericidal/permeability-increasing protein in natural biological fluids / Jerrold Weiss -- Assay systems for measurement of chemotactic activity / H. Anne Pereira -- Neutralization of the in vivo activity of E. coli-derived lipopolysaccharide by cationic peptides / Daniel J. Brackett, Megan R. Lerner, H. Anne Pereira.

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In Antibacterial Peptide Protocols, leading authorities review for the first time in one volume all the major biochemical, molecular, bacteriological, and physical techniques available to assess antimicrobial peptides. These state-of-the-art methods ensure easily reproducible results in such important procedures as the isolation and characterization of antimicrobial peptides, the molecular characterization of genes encoding antimicrobial peptides, and the use of expression systems to isolate peptides. Bioassays and microbial genetic techniques are also included, as are antibacterial assays as the final readout system. These methods detailed in Antibacterial Peptide Protocols will play an important role in the treatment of infectious diseases, particularly with the increasing problem of multidrug-resistant microbes and the relative dearth of new antibiotics being provided by pharmaceutical companies.

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