Basic DNA and RNA protocols / edited by Adrian J. Harwood.

Contributor(s): Harwood, Adrian J
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 58.Publisher: Totowa, N.J. : Humana Press, ©1996Description: 1 online resource (xiii, 514 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592592517; 1592592511; 1280835974; 9781280835971Subject(s): Nucleic acids -- Laboratory manuals | Nucleic Acids | DNA | RNA | Genetic Engineering | Genetic Techniques | Acides nucléiques -- Technique | Acides nucléiques -- Analyse | ADN -- Analyse | ARN -- Analyse | Clonage moléculaire -- Technique | Réaction en chaîne de la polymérase -- Technique | Séquence nucléotidique -- Technique | Mutagenèse dirigée -- Technique | Biologie moléculaire -- Technique | Nucleic acids | DNA | RNA | Wetenschappelijke technieken | Human Anatomy & Physiology | Animal Biochemistry | Health & Biological SciencesGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Basic DNA and RNA protocols.DDC classification: 574.87/328/0724 LOC classification: QP620 | .B38 1996Other classification: 35.75 Online resources: Click here to access online
Contents:
The simultaneous isolation of RNA and DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau -- Restriction endonuclease digestion of DNA / Duncan R. Smith -- Agarose gel electrophoresis / Duncan R. Smith -- Capillary blotting of agarose gels / Duncan R. Smith, David Murphy -- Random primed ³²P-labeling of DNA / Duncan R. Smith -- Hybridization and competition hybridization of Southern blots / Rosemary E. Kelsell -- Utilization of DNA probes with digoxigenin-modified nucleotides in Southern hybridizations / Tim Helentjaris, Tom McCreery -- The preparation of fluorescein-labeled nucleic acid probes and their detection using alkaline phosphatase-catalyzed dioxetane chemiluminescence / Martin Cunningham, Martin Harris -- Monitoring incorporation of fluorescein into nucleic acid probes using a rapid labeling assay / Martin Cunningham -- The preparation of horseradish peroxidase-labeled nucleic acid probes and their detection using enhanced chemiluminescence / Bronwen Harvey, Ian Durrant, Martin Cunningham -- 3'-end labeling of oligonucleotides with fluorescein-11-dUTP and enhanced chemiluminescent detection / Martin Cunningham, Ian Durrant, Bronwen Harvey -- The preparation of riboprobes / Dominique Belin -- Native polyacrylamide gel electrophoresis / Adrian J. Harwood -- Use of silver staining to detect nucleic acids / Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril -- End-labeling of DNA fragments / Adrian J. Harwood -- Northern blot analysis / Robb Krumlauf -- RNA slot blotting / David Murphy -- The RNase protection assay / Dominique Belin -- Primer extension analysis of mRNA / Mark W. Leonard, Roger K. Patient -- S1 mapping using single-stranded DNA probes / Stéphane Viville, Roberto Mantovani -- Nonradioactive in situ hybridization for cells and tissues / Ian Durrant -- In vitro packaging of DNA / Jeremy W. Dale, Peter J. Greenaway -- Construction of mammalian genomic libraries using [lambda] replacement vectors / Alan N. Bateson, Jeffrey W. Pollard -- The production of double-stranded complementary DNA for use in making libraries / Steve Mayall, Jane Kirk -- Construction of cDNA libraries / Michael M. Burrell -- Screening [lambda] libraries / Janet C. Harwood, Adrian J. Harwood -- Subcloning strategies and protocols / Danielle Gioioso Taghian, Jac A. Nickoloff -- Purification of DNA fragments from agarose gels using glass beads / Etienne Joly -- Transformation of E. coli / Fiona M. Tomley -- Transformation of bacteria by electroporation / Lucy Drury -- Preparation of plasmid DNA using alkaline lysis / Etienne Joly -- The rapid boiling method for small-scale preparation of plasmid DNA / Adrian J. Harwood -- Plasmid preparations with diatomaceous earth / Laura Machesky -- Polymerase chain reaction / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White -- Inverse polymerase chain reaction / Daniel L. Hartl, Howard Ochman -- Polymerase chain reaction with degenerate oligonucleotide primers to clone gene family members / Gregory M. Preston -- Cloning PCR products using T-vectors / Michael K. Trower, Greg S. Elgar -- Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer -- A rapid PCR-based colony screening protocol for cloned inserts / Michael K. Trower -- Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem -- Cloning into M13 bacteriophage vectors / Qingzhong Yu -- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff -- M13 phage growth and single-stranded DNA preparation / Fiona M. Tomley -- Preparation of ssDNA from phagemid vectors / Michael K. Trower -- A rapid plasmid purification method for dideoxy sequencing / Annette M. Grifln, Hugh G. Grifln -- DNA sequencing using sequenase version 2.0 T7 DNA polymerase / Carl W. Fuller, Bernard F. McArdle, Annette M. Griffin, Hugh G. Griffin -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Direct sequencing of PCR products / Janet C. Harwood, Geraldine A. Phear -- Thermal cycle dideoxy DNA sequencing / Barton E. Slatko -- Using the automated DNA sequencer / Zijin Du, Richard K. Wilson -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites / Jac A. Nickoloff, Win-Ping Deng, Elizabeth M. Miller, F. Andrew Ray -- A protocol for site-directed mutagenesis employing a uracil-containing phagemid template / Michael K. Trower -- In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / Louise Olliver, Charles D. Boyd -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd -- One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin / Joanne Crowe, Brigitte Steude Masone, Joachim Ribbe.
Action note: digitized 2010 committed to preserveSummary: An essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. The collection includes not only many updated and improved classic techniques, but also a powerful group of advanced methods that point to future progress, among them nonisotopic DNA labeling, silver staining, and automatic sequencing. This excellent bench companion will help those who need to learn for the first time how to conduct research on the molecular biology of nucleic acids or those who need to broaden their competence and laboratory skills. Even highly skilled researchers will find many time-saving techniques.
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Includes bibliographical references and index.

The simultaneous isolation of RNA and DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau -- Restriction endonuclease digestion of DNA / Duncan R. Smith -- Agarose gel electrophoresis / Duncan R. Smith -- Capillary blotting of agarose gels / Duncan R. Smith, David Murphy -- Random primed ³²P-labeling of DNA / Duncan R. Smith -- Hybridization and competition hybridization of Southern blots / Rosemary E. Kelsell -- Utilization of DNA probes with digoxigenin-modified nucleotides in Southern hybridizations / Tim Helentjaris, Tom McCreery -- The preparation of fluorescein-labeled nucleic acid probes and their detection using alkaline phosphatase-catalyzed dioxetane chemiluminescence / Martin Cunningham, Martin Harris -- Monitoring incorporation of fluorescein into nucleic acid probes using a rapid labeling assay / Martin Cunningham -- The preparation of horseradish peroxidase-labeled nucleic acid probes and their detection using enhanced chemiluminescence / Bronwen Harvey, Ian Durrant, Martin Cunningham -- 3'-end labeling of oligonucleotides with fluorescein-11-dUTP and enhanced chemiluminescent detection / Martin Cunningham, Ian Durrant, Bronwen Harvey -- The preparation of riboprobes / Dominique Belin -- Native polyacrylamide gel electrophoresis / Adrian J. Harwood -- Use of silver staining to detect nucleic acids / Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril -- End-labeling of DNA fragments / Adrian J. Harwood -- Northern blot analysis / Robb Krumlauf -- RNA slot blotting / David Murphy -- The RNase protection assay / Dominique Belin -- Primer extension analysis of mRNA / Mark W. Leonard, Roger K. Patient -- S1 mapping using single-stranded DNA probes / Stéphane Viville, Roberto Mantovani -- Nonradioactive in situ hybridization for cells and tissues / Ian Durrant -- In vitro packaging of DNA / Jeremy W. Dale, Peter J. Greenaway -- Construction of mammalian genomic libraries using [lambda] replacement vectors / Alan N. Bateson, Jeffrey W. Pollard -- The production of double-stranded complementary DNA for use in making libraries / Steve Mayall, Jane Kirk -- Construction of cDNA libraries / Michael M. Burrell -- Screening [lambda] libraries / Janet C. Harwood, Adrian J. Harwood -- Subcloning strategies and protocols / Danielle Gioioso Taghian, Jac A. Nickoloff -- Purification of DNA fragments from agarose gels using glass beads / Etienne Joly -- Transformation of E. coli / Fiona M. Tomley -- Transformation of bacteria by electroporation / Lucy Drury -- Preparation of plasmid DNA using alkaline lysis / Etienne Joly -- The rapid boiling method for small-scale preparation of plasmid DNA / Adrian J. Harwood -- Plasmid preparations with diatomaceous earth / Laura Machesky -- Polymerase chain reaction / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White -- Inverse polymerase chain reaction / Daniel L. Hartl, Howard Ochman -- Polymerase chain reaction with degenerate oligonucleotide primers to clone gene family members / Gregory M. Preston -- Cloning PCR products using T-vectors / Michael K. Trower, Greg S. Elgar -- Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer -- A rapid PCR-based colony screening protocol for cloned inserts / Michael K. Trower -- Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem -- Cloning into M13 bacteriophage vectors / Qingzhong Yu -- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff -- M13 phage growth and single-stranded DNA preparation / Fiona M. Tomley -- Preparation of ssDNA from phagemid vectors / Michael K. Trower -- A rapid plasmid purification method for dideoxy sequencing / Annette M. Grifln, Hugh G. Grifln -- DNA sequencing using sequenase version 2.0 T7 DNA polymerase / Carl W. Fuller, Bernard F. McArdle, Annette M. Griffin, Hugh G. Griffin -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Direct sequencing of PCR products / Janet C. Harwood, Geraldine A. Phear -- Thermal cycle dideoxy DNA sequencing / Barton E. Slatko -- Using the automated DNA sequencer / Zijin Du, Richard K. Wilson -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites / Jac A. Nickoloff, Win-Ping Deng, Elizabeth M. Miller, F. Andrew Ray -- A protocol for site-directed mutagenesis employing a uracil-containing phagemid template / Michael K. Trower -- In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / Louise Olliver, Charles D. Boyd -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd -- One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin / Joanne Crowe, Brigitte Steude Masone, Joachim Ribbe.

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An essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. The collection includes not only many updated and improved classic techniques, but also a powerful group of advanced methods that point to future progress, among them nonisotopic DNA labeling, silver staining, and automatic sequencing. This excellent bench companion will help those who need to learn for the first time how to conduct research on the molecular biology of nucleic acids or those who need to broaden their competence and laboratory skills. Even highly skilled researchers will find many time-saving techniques.

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