CDNA library protocols / edited by Ian G. Cowell and Caroline A. Austin.Material type: TextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 69.Publication details: Totowa, N.J. : Humana Press, ©1997. Description: 1 online resource (x, 321 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592595556; 1592595553; 089603383X; 9780896033832; 1280836768; 9781280836763; 9786610836765; 6610836760Subject(s): Gene libraries -- Laboratory manuals | DNA -- Laboratory manuals | Antisense DNA -- Laboratory manuals | Molecular cloning -- Laboratory manuals | Genetic Techniques | DNA, Complementary | Gene Library | DNA, Single-Stranded | DNA Probes | Genetic Structures | Investigative Techniques | Nucleic Acid Probes | DNA | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Genetic Phenomena | Nucleic Acids | Phenomena and Processes | Molecular Probes | Laboratory Chemicals | Nucleic Acids, Nucleotides, and Nucleosides | Chemicals and Drugs | Specialty Uses of Chemicals | Chemical Actions and Uses | Antisense DNA | DNA | Gene libraries | Molecular cloning | DNA | Kloneren | Genetische manipulatie | Wetenschappelijke technieken | complementair dna | complementary dna | dna libraries | biologische technieken | biological techniques | protocollen | protocols | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: CDNA library protocolsDDC classification: 574.87/3282 LOC classification: QH442.4 | .C36 1997NLM classification: W1 | QH 442.4Other classification: 42.20 Online resources: Click here to access online
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Includes bibliographical references and index.
cDNA library construction from small amounts of RNA using paramagnetic beads and PCR / Kris N. Lambert, Valerie M. Williamson -- Increasing the average abundance of low-abundance cDNAs by Ordered subdivision of cDNA populations / David R. Sibson, Michael P. Starkey -- Isolation of messenger RNA from plant tissues / Alison Dunn -- cDNA library construction for the lambda ZAP-based vectors / Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short -- Clone excision methods for the lambda ZAP-based vectors / Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short -- Using rapid amplification of cDNA ends (RACE) to obtain full-length cDNAs / Yue Zhang, Michael A. Frohman -- Inverse PCR approach to cloning cDNA ends / Sheng-He Huang -- Cloning gene family members using the polymerase chain reaction with degenerate oligonucleotide primers / Gregory M. Preston -- Subtractive hybridization for the isolation of differentially expressed genes using magnetic beads / Hans-Christian Aasheim, Ton Logtenberg, Frank Larsen -- Preparation of competent cells for high-efficiency plasmid transformation of Escherichia coli / Yasuhiko Nakata, Xiaoren Tang, Kazunari K. Yokoyama -- The tetramethylammonium chloride (TMAC) method for screening cDNA libraries with highly degenerate oligonucleotide probes obtained by reverse translation of amino acid sequences / Bent Honoé, Peder Madsen -- Screening cDNA libraries by hybridization with double-stranded DNA probes and oligonucleotides / Caroline A. Austin -- Immunological screening of [lambda]-phage cDNA expression libraries / Helen C. Hurst -- Cloning sequence-specific DNA-binding factors from cDNA expression libraries using oligonucleotide binding site probes / Ian G. Cowell -- Protein interaction cloning by far-western screening of [lambda]-phage cDNA expression libraries / Shinichi Takayama, John C. Reed -- Yeast two-hybrid library screening / Ian G. Cowell -- Signal sequence trap : expression cloning method for secreted proteins and Type 1 membrane proteins / Kei Tashiro, Toru Nakano, Tasuku Honjo -- Isolation of genetic suppressor elements (GSEs) from random fragment cDNA libraries in retroviral vectors / Andrei V. Gudkov, Igor B. Roninson -- Expression and preparation of fusion proteins from recombinant [lambda]gt1.1 phages / Sheng-He Huang, Ambrose Jong -- Computer analysis of cloned sequences / Paul R. Caron -- The TIGR human cDNA database / Ewen F. Kirkness, Anthony R. Kerlavage -- Searching the dbEST database / Patricia Rodriguez-Tomé -- Reference cDNA library facilities available from European sources / Michael P. Starkey, Yagnesh Umrania, Christopher R. Mundy, Martin J. Bishop.
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This comprehensive collection of detailed protocols covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely used protocols with some very useful newer techniques. The protocols describe methods for cloning difficult-to-obtain ends of cDNAs, methods for analyzing cDNA sequence data, and methods for using the wealth of cDNA data emerging from the human genome project. Bearing in mind the importance of the library screening method to the determination of cloning strategy, the book offers a wide range of approaches to screening cDNA libraries.