Celiac disease : methods and protocols / edited by Michael N. Marsh.

Includes bibliographical references and index.

Celiac disease : A brief overview / D. Williamson and M.N. Marsh-Genotyping methodologies / S. Bevan and R.S. Houlston-From linkage to genes : Positional cloning / S. Bevan and R.S. Houlston-Linkage and the transmission disequilibrium test in complex traits : Celiac disease as a case study / S. Bevan and R.S. Houlston-Extraction, separation, and purification of wheat gluten proteins and related proteins of barley, rye, and oats / A.S. Tatham [and others]-Structure elucidation of gluten-derived peptides by tandem mass spectrometry / P.A. van Veelen, J.W. Drijfhout and F. Koning-Synthetic peptide libraries for t-cell epitope identification / H.S. Hiemstra, J.W. Drijfhout and F. Koning-Characterization of hla-dq-specific peptide-binding motifs / Y. van de Wal, R. Amons and F. Koning-Studies of gliadin-specific t-cells in celiac disease / O. Molberg [and others]-Morphometric analysis of intestinal mucosa : The measurement of volume compartments and cell volumes in human intestinal mucosa / M.N. Marsh [and others]-Morphometry of rectal mucosa / A. Ensari [and others]-Organ culture of rectal mucosa : In vitro challenge with gluten in celiac disease / G. Mazzarella [and others]-In situ hybridization / C.G. Beckett and P.J. Ciclitira-Measurements of cytokine mrna expression by quantitative polymerase chain reaction in studies of celiac disease / E.M. Nilsen, F.E. Johansen and P. Brandtzaeg-Immunohistochemistry in research and diagnosis of celiac disease / P. Brandtzaeg and F.L. Jahnsen-Immunoassay for detection of iga antitissue transglutaminase in patients with celiac disease / W. Dieterich, E.O. Riecken and D. Schuppan-Antiendomysial and antigliadin antibody tests and diagnosis of celiac disease / D.J. Unsworth-Whole gut lavage fluid analysis : A minimally invasive method for study of mucosal immunity and inflammation / S. Ghosh [and others].

Celiac disease : A brief overview / D. Williamson and M.N. Marsh-Genotyping methodologies / S. Bevan and R.S. Houlston-From linkage to genes : Positional cloning / S. Bevan and R.S. Houlston-Linkage and the transmission disequilibrium test in complex traits : Celiac disease as a case study / S. Bevan and R.S. Houlston-Extraction, separation, and purification of wheat gluten proteins and related proteins of barley, rye, and oats / A.S. Tatham [and others]-Structure elucidation of gluten-derived peptides by tandem mass spectrometry / P.A. van Veelen, J.W. Drijfhout and F. Koning-Synthetic peptide libraries for t-cell epitope identification / H.S. Hiemstra, J.W. Drijfhout and F. Koning-Characterization of hla-dq-specific peptide-binding motifs / Y. van de Wal, R. Amons and F. Koning-Studies of gliadin-specific t-cells in celiac disease / O. Molberg [and others]-Morphometric analysis of intestinal mucosa : The measurement of volume compartments and cell volumes in human intestinal mucosa / M.N. Marsh [and others]-Morphometry of rectal mucosa / A. Ensari [and others]-Organ culture of rectal mucosa : In vitro challenge with gluten in celiac disease / G. Mazzarella [and others]-In situ hybridization / C.G. Beckett and P.J. Ciclitira-Measurements of cytokine mrna expression by quantitative polymerase chain reaction in studies of celiac disease / E.M. Nilsen, F.E. Johansen and P. Brandtzaeg-Immunohistochemistry in research and diagnosis of celiac disease / P. Brandtzaeg and F.L. Jahnsen-Immunoassay for detection of iga antitissue transglutaminase in patients with celiac disease / W. Dieterich, E.O. Riecken and D. Schuppan-Antiendomysial and antigliadin antibody tests and diagnosis of celiac disease / D.J. Unsworth-Whole gut lavage fluid analysis : A minimally invasive method for study of mucosal immunity and inflammation / S. Ghosh [and others].

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With the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.

English.