Mammalian artificial chromosomes : methods and protocols / edited by Vittorio Sgaramella and Sandro Eridani.

Contributor(s): Sgaramella, V | Eridani, Sandro
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 240.Publisher: Totowa, N.J. : Humana Press, ©2004Description: 1 online resource (xi, 269 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 1592594344; 9781592594344; 1588290964; 9781588290960Subject(s): Mammalian artificial chromosomes -- Synthesis -- Laboratory manuals | Chromosomes, Artificial, Mammalian | Genetic Techniques | SCIENCE -- BiotechnologyGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Mammalian artificial chromosomes.DDC classification: 660.6/5 LOC classification: QH600.15 | .M35 2004Online resources: Click here to access online
Contents:
From natural to artificial chromosomes: An overview / V. Sgaramella and S. Eridani -- Methodologies applied to domestic animal chromosomes / L. Iannuzzi -- Mapping of the hotspots of recombination in human DNA cloned as yeast artificial chromosomes / G. Ira, J. Krol and J. Filipski -- Genomic imprinting and its effects on genes and chromosomes in mammals / Y. Goto and R. Feil -- Centromeres and neocentromeres / G. Roizes [and others] -- Identification of start sites of bi-directional DNA synthesis at eukaryotic DNA replication origins / A. Vindigni, G. Abdurashidova and A. Falaschi -- Telomere length analysis and in vitro telomerase assay / F. Ascenzioni, P.A. Fradiani and P. Donini -- Telomerization of mammalian cells and transplantation of telomerized cells in immunodeficient mice / P.J. Hornsby -- Chromosome-based vectors for mammalian cells: An overview / H.N. Lim and C.J. Farr -- Mammalian artificial chromosome formation in human cells after lipofection of a pac precursor / J.I. de las Heras, L. D'Aiuto and H. Cooke -- Use of natural and artificial chromosome vectors for animal transgenesis / Y. Kuroiwa, K. Tomizuka and I. Ishida -- Pronuclear microinjection of purified artificial chromosomes for generation of transgenic mice: Pick-and-inject technique / D.P. Monteith [and others] -- Modification of human bacterial artificial chromosome clones for functional studies and therapeutic applications / M.R. Orford [and others].
In: Springer ProtocolsSummary: Assembly of the basic units of mammalian chromosomes into artificial constructs, and the reduction of their components to more manageable dimensions, has now become a reality. In Mammalian Artificial Chromosomes: Methods and Protocols, leading scientists in cell biology, genetics, cell culturing, and gene transfer present their most productive methods for the preparation, characterization, and use of artificial chromosomes. The authors cover all relevant areas of artificial chromosome research-from basic genetics to daring attempts at building new gene therapy tools-and describe possible applications in genetic manipulation requiring large portions of the genome. Each fully tested protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, reagent and equipment lists, and tips on troubleshooting and how to avoid pitfalls. Cutting-edge and much-needed, Mammalian Artificial Chromosomes: Methods and Protocols offers a timely collection of meticulous techniques that will help elucidate the mechanisms of nuclear duplication, allow the introduction of changes into the human genome, and revolutionize our understanding of how chromosomes work.
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Includes bibliographical references and index.

From natural to artificial chromosomes: An overview / V. Sgaramella and S. Eridani -- Methodologies applied to domestic animal chromosomes / L. Iannuzzi -- Mapping of the hotspots of recombination in human DNA cloned as yeast artificial chromosomes / G. Ira, J. Krol and J. Filipski -- Genomic imprinting and its effects on genes and chromosomes in mammals / Y. Goto and R. Feil -- Centromeres and neocentromeres / G. Roizes [and others] -- Identification of start sites of bi-directional DNA synthesis at eukaryotic DNA replication origins / A. Vindigni, G. Abdurashidova and A. Falaschi -- Telomere length analysis and in vitro telomerase assay / F. Ascenzioni, P.A. Fradiani and P. Donini -- Telomerization of mammalian cells and transplantation of telomerized cells in immunodeficient mice / P.J. Hornsby -- Chromosome-based vectors for mammalian cells: An overview / H.N. Lim and C.J. Farr -- Mammalian artificial chromosome formation in human cells after lipofection of a pac precursor / J.I. de las Heras, L. D'Aiuto and H. Cooke -- Use of natural and artificial chromosome vectors for animal transgenesis / Y. Kuroiwa, K. Tomizuka and I. Ishida -- Pronuclear microinjection of purified artificial chromosomes for generation of transgenic mice: Pick-and-inject technique / D.P. Monteith [and others] -- Modification of human bacterial artificial chromosome clones for functional studies and therapeutic applications / M.R. Orford [and others].

Print version record.

Assembly of the basic units of mammalian chromosomes into artificial constructs, and the reduction of their components to more manageable dimensions, has now become a reality. In Mammalian Artificial Chromosomes: Methods and Protocols, leading scientists in cell biology, genetics, cell culturing, and gene transfer present their most productive methods for the preparation, characterization, and use of artificial chromosomes. The authors cover all relevant areas of artificial chromosome research-from basic genetics to daring attempts at building new gene therapy tools-and describe possible applications in genetic manipulation requiring large portions of the genome. Each fully tested protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, reagent and equipment lists, and tips on troubleshooting and how to avoid pitfalls. Cutting-edge and much-needed, Mammalian Artificial Chromosomes: Methods and Protocols offers a timely collection of meticulous techniques that will help elucidate the mechanisms of nuclear duplication, allow the introduction of changes into the human genome, and revolutionize our understanding of how chromosomes work.

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