In situ hybridization protocols / edited by Ian A. Darby.

Contributor(s): Darby, Ian A
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): volume 123.Publisher: Totowa, N.J. : Humana Press, 2000Edition: 2nd edDescription: 1 online resource (xiii, 343 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592596775; 1592596770Subject(s): In situ hybridization -- Laboratory manuals | Situ Hybridization | Hybridation in situ -- Manuels de laboratoire | SCIENCE -- Life Sciences -- Molecular Biology | In situ hybridization | Wetenschappelijke technieken | In-situ-Hybridisierung | AufsatzsammlungGenre/Form: Laboratory Manual. | Electronic books. | Handbooks and manuals. Additional physical formats: Print version:: In situ hybridization protocols.DDC classification: 572.8/4 LOC classification: QH452.8 | .I54 2000Online resources: Click here to access online
Contents:
pt. 1. Chromosomal techniques -- Preparation of human partial chromosome paints from somatic cell hybirds -- DNA-protein In Situ convisualization for chromosome analysis -- Radioactive In Situ hybridization to animal chromosomes -- Native polytene chromosomes of Drosophila melanogaster for light and electron microscopic observation of the conformation and distribution of molecules -- Released chromatin or DNA fiber perparations for high-resolution fiber FISH -- In Situ hybridization to polytene chromosomes of Drosophila melanogaster and other dipteran species -- In Situ hybridization to polytene chromosomes -- Comparative gene mapping in exotic species using FISH -- pt. 2. Tissue techniques -- In Situ hybridization with nonradiaoactive probes -- Quantitative In Situ hybridization using radioactive probes to study gene expression in heterocellular systems -- In Situ end-labeling of fragmented DNA and the localization of apoptosis -- Combined immunohistochemical labeling and In Situ hybridization to colocalize mRNA and protein in tissue sections -- In Situ hybridization protocols for detection of viral DNA using radioactive and nonradioactive DNA probes -- Signal amplification for DNA and mRNA -- In Situ localization of PCR-amplified DNA and cDNA -- Ultrastructural detection of nucleic acids on thin sections of tissue embedded in hydrophilic resin -- In Situ hybridization for electron microscopy -- In Situ hybridization of whole-mount embryos -- cRNA probes: comparison of isotopic and nonisotopic detection methods -- pt. 3. Applications -- Molecular cytogenetic analysis of sperm from infertile males undergoing intracytoplasmic sperm injection -- Localization of HIV-1 DNA and tumor necrosis factor-[alpha] mRNA in human brain using polymerase chain reaction In Situ hybridization and immunocytochemistry.
Summary: InIn Situ Hybridization Protocols, 2nd Edition, Ian Darby updates the highly successful 1st edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques-many not covered in the first edition-and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Each protocol provides detailed, easy-to-follow instructions, along with realistic commentaries on how to vary their application and how to troubleshoot problems that arise. Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire.
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Includes bibliographical references and index.

pt. 1. Chromosomal techniques -- Preparation of human partial chromosome paints from somatic cell hybirds -- DNA-protein In Situ convisualization for chromosome analysis -- Radioactive In Situ hybridization to animal chromosomes -- Native polytene chromosomes of Drosophila melanogaster for light and electron microscopic observation of the conformation and distribution of molecules -- Released chromatin or DNA fiber perparations for high-resolution fiber FISH -- In Situ hybridization to polytene chromosomes of Drosophila melanogaster and other dipteran species -- In Situ hybridization to polytene chromosomes -- Comparative gene mapping in exotic species using FISH -- pt. 2. Tissue techniques -- In Situ hybridization with nonradiaoactive probes -- Quantitative In Situ hybridization using radioactive probes to study gene expression in heterocellular systems -- In Situ end-labeling of fragmented DNA and the localization of apoptosis -- Combined immunohistochemical labeling and In Situ hybridization to colocalize mRNA and protein in tissue sections -- In Situ hybridization protocols for detection of viral DNA using radioactive and nonradioactive DNA probes -- Signal amplification for DNA and mRNA -- In Situ localization of PCR-amplified DNA and cDNA -- Ultrastructural detection of nucleic acids on thin sections of tissue embedded in hydrophilic resin -- In Situ hybridization for electron microscopy -- In Situ hybridization of whole-mount embryos -- cRNA probes: comparison of isotopic and nonisotopic detection methods -- pt. 3. Applications -- Molecular cytogenetic analysis of sperm from infertile males undergoing intracytoplasmic sperm injection -- Localization of HIV-1 DNA and tumor necrosis factor-[alpha] mRNA in human brain using polymerase chain reaction In Situ hybridization and immunocytochemistry.

InIn Situ Hybridization Protocols, 2nd Edition, Ian Darby updates the highly successful 1st edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques-many not covered in the first edition-and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Each protocol provides detailed, easy-to-follow instructions, along with realistic commentaries on how to vary their application and how to troubleshoot problems that arise. Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire.

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