Nonviral vectors for gene therapy : methods and protocols / edited by Mark A. Findeis.
Contributor(s): Findeis, Mark AMaterial type: TextSeries: Methods in molecular medicine: 65.Publisher: Totowa, N.J. : Humana Press, ©2001Description: 1 online resource (xi, 399 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592591398; 1592591396; 0896037126; 9780896037120Subject(s): Gene therapy -- Laboratory manuals | Genetic vectors -- Laboratory manuals | HEALTH & FITNESS -- Diseases -- Genetic | MEDICAL -- Genetics | Gene therapy | Genetic vectors | Gentherapie | Wetenschappelijke technieken | Medicine | Health & Biological Sciences | Pathology | Investigative Techniques | Biological Therapy | Genetic Structures | Genetic Techniques | Genetic Engineering | Drug Therapy | Therapeutics | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Genetic Phenomena | Phenomena and Processes | Genetic Therapy | Methods | Drug Delivery Systems | Gene Transfer Techniques | Genetic VectorsGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Nonviral vectors for gene therapyDDC classification: 616/.042 LOC classification: RB155.8 | .N665 2001Other classification: 44.48 Online resources: Click here to access online
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Includes bibliographical references and index.
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Nonviral gene therapy-an emerging field with great clinical promise-avoids many problems associated with viral gene therapy and offers the possibility of superior clinical effectiveness. In Nonviral Vectors for Gene Therapy: Methods and Protocols, Mark A. Findeis has assembled a panel of active researchers to present their best methods not only for preparing, handling, and characterizing gene delivery agents, but also for gene delivery. To help those preparing and characterizing gene transfer agents, the contributors examine a broad range of compounds that bind with DNA to form the compact complexes that facilitate cellular delivery-among them peptide conjugates, synthetic polymers, and lipids. They also outline specific approaches to gene transfer in vivo, including direct delivery by intratumoral injection and indirect delivery by cell-specific targeting of DNA complexes, and discuss in detail many spectroscopic techniques for characterizing nonviral gene delivery agents. Opportunities for the development and application of these novel vectors in the research lab and eventually, in the clinic, are highlighted. Comprehensive and state-of-the-art, Nonviral Vectors for Gene Therapy: Methods and Protocols illuminates for today's investigators the powerful new approaches to the creation of nonviral materials, as well as their efficacious use in delivering therapeutic genes to patients across the spectrum of human disease.
""Nonviral Vectors for Gene Therapy""; ""Preface""; ""Contents""; ""Contributors""; ""1 Synthesis of Polyampholyte Comb-Type Copolymers Consisting of Poly(L-lysine) Backbone and Hyaluronic Acid Side Chains for DNA Carrier""; ""2 Cationic alpha-Helical Peptides for Gene Delivery into Cells""; ""3 Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly(L-lysine) Dendrimer with Plasmid DNA""; ""4 Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery""; ""5 Stabilization of Polycationâ€?DNA Complexes by Surface Modification with Hydrophilic Polymers""
""6 Use of Disulfide Cationic Lipids in Plasmid DNA Delivery""""7 Interactions of Lipidâ€?Oligonucleotide Conjugates with Low-Density Lipoprotein""; ""8 Coupling of Nuclear Localization Signals to Plasmid DNA""; ""9 Progress Toward a Synthetic Virus: A Multicomponent System for Liver-Directed DNA Delivery""; ""10 Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers""; ""11 Methods for Studying Formation of Polycationâ€?DNA Complexes and Properties Useful for Gene Delivery""; ""12 Characterization of DNA Condensates by Atomic Force Microscopy""
""13 Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitting""""14 Systemic Delivery of Therapeutic Proteins by Intramuscular Injection of Plasmid DNA""; ""15 Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNAâ€?Lipid Complexes""; ""16 Nonviral DNA Delivery from Polymeric Systems""; ""17 Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers""; ""18 Lyophilization of Nonviral Gene Delivery Systems""
""19 Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes""""20 Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy""; ""21 Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry""; ""22 Light-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors""; ""23 Renal Gene Therapy""; ""Index""