Pulsed-field gel electrophoresis / edited by Margit Burmeister, Levy Ulanovsky.

Contributor(s): Burmeister, Margit | Ulanovsky, Levy
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 12.Publisher: Totowa, N.J. : Humana Press, ©1992Description: 1 online resource (xiii, 481 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592594993; 1592594999Subject(s): Pulsed-field gel electrophoresis -- Technique | DNA -- Analysis | Electrophoresis, Gel, Pulsed-Field -- methods | Genetic Techniques | DNA -- Analysis | Pulsed-field gel electrophoresis -- Technique | Elektroforese | Gel | Wetenschappelijke techniekenGenre/Form: Electronic books. Additional physical formats: Print version:: Pulsed-field gel electrophoresis.DDC classification: 574.8/8/028 LOC classification: QP519.9.P84 | P85 1992Other classification: 35.20 Online resources: Click here to access online
Contents:
Field-inversion gel electrophoresis / Georges F. Carle -- Resolving multimegabase DNA molecules using contour-clamped homogeneous electric fields (CHEF) / Douglas Vollrath -- Separation and size determination of DNA over a 10-200 kbp range / Craig A. Cooney -- PFGE using double-inhomogeneous fields or orthogonal field-alternating gel electrophoresis (OFAGE) / Margit Burmeister -- Transverse alternating-field electrophoresis / Katheleen Gardiner -- Rotating field gel electrophoresis (ROFE) / Andreas Ziegler and Armin Volz -- Preparation, manipulation, and pulse strategy for one-dimensional pulsed-field gel electrophoresis (ODPFGE) / Jaan Noolandi and Chantal Turmel -- Preparation, restriction, and hybridization analysis of mammalian genomic DNA for pulsed-field gel electrophoresis / Denise P. Barlow -- Encapsulation of cells in Agarose Beads / Joan Overhauser -- Preparation and separation of intact chromosomes of vertebrates by one-dimensional pulsed-field gel electrophoresis (ODPFGE) / Jaan Noolandi and Chantal Turmel -- Isolation of plant DNA for pulsed-field gel electrophoresis / Kun-Sheng Wu, Marion S. Roder, and Martin W. Ganal -- Methyltransferases as tools to alter the specificity of restriction endonucleases / Bruno W.S. Sobral and Michael McClelland -- Desktop digital imaging: application to detection of length heterogeneity after hyperresonant pulsed-field gel electrophoresis of mature bacteriophage P22 DNA / Gary A. Griess, Elena T. Moreno, and Philip Serwer -- Bacterial genome mapping by two-dimensional pulsed-field gel electrophoresis (2D-PFGE) / Wilfried Bautsch -- Protozoan genomes: karyotype analysis, chromosome structure, and chromosome specific libraries / Lex H.T. Van der Ploeg [and others] -- Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis / Anthony P. Monaco, Zoia Larin, and Hans Lehrach -- Analysis of yeast artificial chromosome clones / Settara C. Chandrasekharappa, Douglas A. Marchuk, and Francis S. Collins -- Strategies for mapping large regions of mammalian genomes / Margit Burmeister -- Two-dimensional DNA electrophoresis (2D-DE) for mammalian DNA / Michael A. Walter and Diane W. Cox -- Pulsed-field and two-dimensional gel electrophoresis of long arrays of tandemly repeated DNA: analysis of human centromeric alpha satellite / Peter E. Warburton [and others] -- Construction of Lamda libraries from large PFGE fragments / Catrin Pritchard and Margit Burmeister -- In-gel detection of DNA: application to study of viral DNA metabolism by use of pulsed-field agarose gel electrophoresis / Philip Serwer, Robert H. Watson, and Marjatta Son -- Theories of pulsed-field gel electrophoresis / Stephen D. Levene -- The dynamics of DNA gel electrophoresis / Joshua M. Deutsch -- Orientation of the agarose matrix by pulsed electric fields / Nancy C. Stellwagen -- Reptation theory of pulsed electrophoresis and trapping electrophoresis / Jean Louis Viovy and Anne Dominique Defontaines -- Elastic bag model of one-dimensional pulsed-field gel electrophoresis (ODPFGE) / Jaan Noolandi and Chantal Turmel.
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Includes bibliographical references and index.

Field-inversion gel electrophoresis / Georges F. Carle -- Resolving multimegabase DNA molecules using contour-clamped homogeneous electric fields (CHEF) / Douglas Vollrath -- Separation and size determination of DNA over a 10-200 kbp range / Craig A. Cooney -- PFGE using double-inhomogeneous fields or orthogonal field-alternating gel electrophoresis (OFAGE) / Margit Burmeister -- Transverse alternating-field electrophoresis / Katheleen Gardiner -- Rotating field gel electrophoresis (ROFE) / Andreas Ziegler and Armin Volz -- Preparation, manipulation, and pulse strategy for one-dimensional pulsed-field gel electrophoresis (ODPFGE) / Jaan Noolandi and Chantal Turmel -- Preparation, restriction, and hybridization analysis of mammalian genomic DNA for pulsed-field gel electrophoresis / Denise P. Barlow -- Encapsulation of cells in Agarose Beads / Joan Overhauser -- Preparation and separation of intact chromosomes of vertebrates by one-dimensional pulsed-field gel electrophoresis (ODPFGE) / Jaan Noolandi and Chantal Turmel -- Isolation of plant DNA for pulsed-field gel electrophoresis / Kun-Sheng Wu, Marion S. Roder, and Martin W. Ganal -- Methyltransferases as tools to alter the specificity of restriction endonucleases / Bruno W.S. Sobral and Michael McClelland -- Desktop digital imaging: application to detection of length heterogeneity after hyperresonant pulsed-field gel electrophoresis of mature bacteriophage P22 DNA / Gary A. Griess, Elena T. Moreno, and Philip Serwer -- Bacterial genome mapping by two-dimensional pulsed-field gel electrophoresis (2D-PFGE) / Wilfried Bautsch -- Protozoan genomes: karyotype analysis, chromosome structure, and chromosome specific libraries / Lex H.T. Van der Ploeg [and others] -- Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis / Anthony P. Monaco, Zoia Larin, and Hans Lehrach -- Analysis of yeast artificial chromosome clones / Settara C. Chandrasekharappa, Douglas A. Marchuk, and Francis S. Collins -- Strategies for mapping large regions of mammalian genomes / Margit Burmeister -- Two-dimensional DNA electrophoresis (2D-DE) for mammalian DNA / Michael A. Walter and Diane W. Cox -- Pulsed-field and two-dimensional gel electrophoresis of long arrays of tandemly repeated DNA: analysis of human centromeric alpha satellite / Peter E. Warburton [and others] -- Construction of Lamda libraries from large PFGE fragments / Catrin Pritchard and Margit Burmeister -- In-gel detection of DNA: application to study of viral DNA metabolism by use of pulsed-field agarose gel electrophoresis / Philip Serwer, Robert H. Watson, and Marjatta Son -- Theories of pulsed-field gel electrophoresis / Stephen D. Levene -- The dynamics of DNA gel electrophoresis / Joshua M. Deutsch -- Orientation of the agarose matrix by pulsed electric fields / Nancy C. Stellwagen -- Reptation theory of pulsed electrophoresis and trapping electrophoresis / Jean Louis Viovy and Anne Dominique Defontaines -- Elastic bag model of one-dimensional pulsed-field gel electrophoresis (ODPFGE) / Jaan Noolandi and Chantal Turmel.

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