Renal cancer : methods and protocols / edited by Jack H. Mydlo.

Contributor(s): Mydlo, Jack H
Material type: TextTextSeries: Methods in molecular medicine: 53.Publisher: Totowa, N.J. : Humana Press, ©2001Description: 1 online resource (xii, 403 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592591442; 1592591442; 0896038289; 9780896038288; 1280821094; 9781280821097; 9786610821099; 6610821097Subject(s): Kidneys -- Cancer -- Molecular aspects | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Kidney Diseases | Urologic Neoplasms | Adenocarcinoma | Urologic Diseases | Carcinoma | Urogenital Neoplasms | Female Urogenital Diseases | Male Urogenital Diseases | Neoplasms, Glandular and Epithelial | Neoplasms by Site | Neoplasms | Female Urogenital Diseases and Pregnancy Complications | Neoplasms by Histologic Type | Disease | Kidney Neoplasms | Carcinoma, Renal Cell | Diagnosis | MEDICAL -- Oncology | HEALTH & FITNESS -- Diseases -- Cancer | Kidneys -- Cancer -- Molecular aspects | Wetenschappelijke techniekenGenre/Form: Electronic books. Additional physical formats: Renal cancer.DDC classification: 616.99/461 LOC classification: RC280.K5 | R455 2001Online resources: Click here to access online
Contents:
Renal cell carcinoma / P. Russo -- Pathology of kidney tumors / D.J. Grignon -- Telomerase assay in renal cancer / W.W. Zhang and L.H. Klotz -- Comparative genomic hybridization / J.C. Presti -- Analyzing the FHIT gene by RT-PCR, western blotting, and immunohistochemistry / R. Baffa [and others] -- Detection of apoptosis in renal cell carcinoma / K.J. Pienta and J.F. Williams -- Fluorescence in situ hybridization (FISH) to metaphase and interphase chromosomes / J.A. Macoska -- Polymerase chain reaction detection of DNA sequence deletions / J.A. Macoska -- Matrix metalloproteinases and their inhibitors / A. Kugler, P. Thelen and R.-H. Ringert -- Purification of growth factor mRNA in renal tissues : bFGF-2, FGF-2, TGF [alpha], and EGFR / J.H. Mydlo -- Laser-capture microdissection / G.L. Griewe [and others] -- Expression of c-met and WT-1 / L. Kushner [and others] -- Molecular analysis of the von Hippel-Lindau disease gene / A. Chernoff [and others] -- Experimental models and methods in antibody targeting of renal cell carcinoma / R.L. Vessella [and others] -- Orthotopic model of renal cell carcinoma / D.M. Nanus and D. Engelstein -- Murine animal model / N. Nishisaka [and others] -- Angiogenesis assays / J.H. Mydlo -- Antiangiogenic approaches to renal cell carcinoma / S.C. Campbell, F.K. Reiher and N.D. Smith -- Interferon alpha, GM-CSF-activated T cells, and IL-6 in renal cell carcinoma / B. Patel and A. Belldegrun -- Immunotherapeutic approach to cancer with cutaneous DNA vaccination / W.B. Browne, W.G. Hawkins and J.J. Lewis -- Monoclonal antibodies / E. Oosterwijk -- Treatment strategies in metastatic renal cell carcinoma / P.F. Mulders -- 23assessment of T-cell immune dysfunction in patients with renal cell carcinoma / R.G. Uzzo [and others] -- Transforming growth factor-[Beta]1 as a novel marker of response to therapy for renal cell carcinoma / H.L. Adler -- Static and flow cytometry / R. Madeb, D. Pode and O. Nativ.
Renal cell carcinoma / P. Russo -- Pathology of kidney tumors / D.J. Grignon -- Telomerase assay in renal cancer / W.W. Zhang and L.H. Klotz -- Comparative genomic hybridization / J.C. Presti -- Analyzing the FHIT gene by RT-PCR, western blotting, and immunohistochemistry / R. Baffa [and others] -- Detection of apoptosis in renal cell carcinoma / K.J. Pienta and J.F. Williams -- Fluorescence in situ hybridization (FISH) to metaphase and interphase chromosomes / J.A. Macoska -- Polymerase chain reaction detection of DNA sequence deletions / J.A. Macoska -- Matrix metalloproteinases and their inhibitors / A. Kugler, P. Thelen and R.-H. Ringert -- Purification of growth factor mRNA in renal tissues : [Beta]FGF-2, FGF-2, TGF [Alpha], and EGFR / J.H. Mydlo -- Laser-capture microdissection / G.L. Griewe [and others] -- Expression of c-met and WT-1 / L. Kushner [and others] -- Molecular analysis of the von Hippel-Lindau disease gene / A. Chernoff [and others] -- Experimental models and methods in antibody targeting of renal cell carcinoma / R.L. Vessella [and others] -- Orthotopic model of renal cell carcinoma / D.M. Nanus and D. Engelstein -- Murine animal model / N. Nishisaka [and others] -- Angiogenesis assays / J.H. Mydlo -- Antiangiogenic approaches to renal cell carcinoma / S.C. Campbell, F.K. Reiher and N.D. Smith -- Interferon alpha, GM-CSF-activated T cells, and IL-6 in renal cell carcinoma / B. Patel and A. Belldegrun -- Immunotherapeutic approach to cancer with cutaneous DNA vaccination / W.B. Browne, W.G. Hawkins and J.J. Lewis -- Monoclonal antibodies / E. Oosterwijk -- Treatment strategies in metastatic renal cell carcinoma / P.F. Mulders -- 23assessment of T-cell immune dysfunction in patients with renal cell carcinoma / R.G. Uzzo [and others] -- Transforming growth factor-[Beta]1 as a novel marker of response to therapy for renal cell carcinoma / H.L. Adler -- Static and flow cytometry / R. Madeb, D. Pode and O. Nativ.
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: Because renal cancer can be managed successfully only when localized, there is a great need to develop new treatments for patients with advanced or metastatic disease. In Renal Cancer: Methods and Protocols, Jack H. Mydlo, MD, and a panel of leading clinicians and researchers review every aspect of the latest surgical, medical, and immunological therapies that can be used in the diagnosis and treatment of renal cancer. These broadly experienced investigators also present a practical account of their best basic research methods, including the use of reverse transcriptase PCR combined with genomic hybridization, cadherin, and metalloproteinase expression to reveal important factors in the detection, staging, aggressiveness, and treatment of this disease. Gene therapy, the generation of monoclonal antibodies, and the use of interferon alpha, GM-CSF, and IL-6 are also discussed. In vivo assays are provided for analyzing angiogenesis, anti-angiogenesis, and general renal tumor biology as a prelude to human clinical trials. Comprehensive and pioneering, Renal Cancer: Methods and Protocols offers urologists, medical oncologists, laboratory investigators, and pathologists a practical collection of the major cutting-edge techniques and therapies for renal cancer today, together with a view of the highly promising future of gene therapy.
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Includes bibliographical references and index.

Renal cell carcinoma / P. Russo -- Pathology of kidney tumors / D.J. Grignon -- Telomerase assay in renal cancer / W.W. Zhang and L.H. Klotz -- Comparative genomic hybridization / J.C. Presti -- Analyzing the FHIT gene by RT-PCR, western blotting, and immunohistochemistry / R. Baffa [and others] -- Detection of apoptosis in renal cell carcinoma / K.J. Pienta and J.F. Williams -- Fluorescence in situ hybridization (FISH) to metaphase and interphase chromosomes / J.A. Macoska -- Polymerase chain reaction detection of DNA sequence deletions / J.A. Macoska -- Matrix metalloproteinases and their inhibitors / A. Kugler, P. Thelen and R.-H. Ringert -- Purification of growth factor mRNA in renal tissues : bFGF-2, FGF-2, TGF [alpha], and EGFR / J.H. Mydlo -- Laser-capture microdissection / G.L. Griewe [and others] -- Expression of c-met and WT-1 / L. Kushner [and others] -- Molecular analysis of the von Hippel-Lindau disease gene / A. Chernoff [and others] -- Experimental models and methods in antibody targeting of renal cell carcinoma / R.L. Vessella [and others] -- Orthotopic model of renal cell carcinoma / D.M. Nanus and D. Engelstein -- Murine animal model / N. Nishisaka [and others] -- Angiogenesis assays / J.H. Mydlo -- Antiangiogenic approaches to renal cell carcinoma / S.C. Campbell, F.K. Reiher and N.D. Smith -- Interferon alpha, GM-CSF-activated T cells, and IL-6 in renal cell carcinoma / B. Patel and A. Belldegrun -- Immunotherapeutic approach to cancer with cutaneous DNA vaccination / W.B. Browne, W.G. Hawkins and J.J. Lewis -- Monoclonal antibodies / E. Oosterwijk -- Treatment strategies in metastatic renal cell carcinoma / P.F. Mulders -- 23assessment of T-cell immune dysfunction in patients with renal cell carcinoma / R.G. Uzzo [and others] -- Transforming growth factor-[Beta]1 as a novel marker of response to therapy for renal cell carcinoma / H.L. Adler -- Static and flow cytometry / R. Madeb, D. Pode and O. Nativ.

Renal cell carcinoma / P. Russo -- Pathology of kidney tumors / D.J. Grignon -- Telomerase assay in renal cancer / W.W. Zhang and L.H. Klotz -- Comparative genomic hybridization / J.C. Presti -- Analyzing the FHIT gene by RT-PCR, western blotting, and immunohistochemistry / R. Baffa [and others] -- Detection of apoptosis in renal cell carcinoma / K.J. Pienta and J.F. Williams -- Fluorescence in situ hybridization (FISH) to metaphase and interphase chromosomes / J.A. Macoska -- Polymerase chain reaction detection of DNA sequence deletions / J.A. Macoska -- Matrix metalloproteinases and their inhibitors / A. Kugler, P. Thelen and R.-H. Ringert -- Purification of growth factor mRNA in renal tissues : [Beta]FGF-2, FGF-2, TGF [Alpha], and EGFR / J.H. Mydlo -- Laser-capture microdissection / G.L. Griewe [and others] -- Expression of c-met and WT-1 / L. Kushner [and others] -- Molecular analysis of the von Hippel-Lindau disease gene / A. Chernoff [and others] -- Experimental models and methods in antibody targeting of renal cell carcinoma / R.L. Vessella [and others] -- Orthotopic model of renal cell carcinoma / D.M. Nanus and D. Engelstein -- Murine animal model / N. Nishisaka [and others] -- Angiogenesis assays / J.H. Mydlo -- Antiangiogenic approaches to renal cell carcinoma / S.C. Campbell, F.K. Reiher and N.D. Smith -- Interferon alpha, GM-CSF-activated T cells, and IL-6 in renal cell carcinoma / B. Patel and A. Belldegrun -- Immunotherapeutic approach to cancer with cutaneous DNA vaccination / W.B. Browne, W.G. Hawkins and J.J. Lewis -- Monoclonal antibodies / E. Oosterwijk -- Treatment strategies in metastatic renal cell carcinoma / P.F. Mulders -- 23assessment of T-cell immune dysfunction in patients with renal cell carcinoma / R.G. Uzzo [and others] -- Transforming growth factor-[Beta]1 as a novel marker of response to therapy for renal cell carcinoma / H.L. Adler -- Static and flow cytometry / R. Madeb, D. Pode and O. Nativ.

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Because renal cancer can be managed successfully only when localized, there is a great need to develop new treatments for patients with advanced or metastatic disease. In Renal Cancer: Methods and Protocols, Jack H. Mydlo, MD, and a panel of leading clinicians and researchers review every aspect of the latest surgical, medical, and immunological therapies that can be used in the diagnosis and treatment of renal cancer. These broadly experienced investigators also present a practical account of their best basic research methods, including the use of reverse transcriptase PCR combined with genomic hybridization, cadherin, and metalloproteinase expression to reveal important factors in the detection, staging, aggressiveness, and treatment of this disease. Gene therapy, the generation of monoclonal antibodies, and the use of interferon alpha, GM-CSF, and IL-6 are also discussed. In vivo assays are provided for analyzing angiogenesis, anti-angiogenesis, and general renal tumor biology as a prelude to human clinical trials. Comprehensive and pioneering, Renal Cancer: Methods and Protocols offers urologists, medical oncologists, laboratory investigators, and pathologists a practical collection of the major cutting-edge techniques and therapies for renal cancer today, together with a view of the highly promising future of gene therapy.

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