SV40 protocols / edited by Leda Raptis.

Contributor(s): Raptis, Leda
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 165.Publisher: Totowa, N.J. : Humana Press, 2001Description: 1 online resource (xi, 310 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 0896036537; 9780896036536; 1592591175; 9781592591176Other title: Simian virus 40 protocolsSubject(s): SV40 (Virus) -- Laboratory manuals | Simian virus 40 -- genetics | Genetic Techniques | SCIENCE -- Life Sciences -- Biology | SCIENCE -- Life Sciences -- Microbiology | SV40 (Virus)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: SV40 protocolsDDC classification: 579.2/445 LOC classification: QR406.2.S56 | S87 2001Online resources: Click here to access online
Contents:
Propagation of wild-type and mutant SV40 -- Propagation and assay of the JC virus -- Propagation and assay of BK virus -- Molecular methods for identification and genotyping of BK virus -- Methods for studying interactions between simian virus 40 T-antigen and the viral origin of replication -- Cell-free transcription of SV40 -- Quantitation and structural analysis of SV40 RNAs -- Generation of recombinant SV40 vectors for gene transfer -- Production of SV40 proteins in insect cells and in vitro packaging of virions and pseduovirions -- Using retroviral vectors to express SV40 tumor antigens -- Neoplastic transformation assays -- Methods to assess inhibition of adipocyte dfferentiation by SV40 large T antigen -- SV40-mediated immortalization -- Biology of p53 and SV40 large T association -- Binding of SV40 large T antigen to the retinoblastoma susceptibility gene product and related proteins -- In situ electroporation for the measurement of c-Ras activation by SVLT -- Role of SV40 small t in cell lysis, transformation, and signaling -- Cytotoxic T lymphocytes in SV40 infections -- Detection of SV40 DNA sequences in human tissue -- Guide to techniques in creating transgenic mouse models using SV40 T antigen.
Summary: Extensive research has shown that Simian Virus 40, a contaminant of polio and adenovirus vaccines that may be implicated in human cancers, can also serve as a powerful probe for examining many fundamental questions in molecular biology. In SV40 Protocols, Leda Raptis and a panel of highly experienced investigators describe in step-by-step fashion key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and its related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for the use of retroviral vectors to express SV40 tumor antigens in cultured cells, and for transgenic mouse models based on the SV40 large T antigen. All methods have been optimized for experimental success, and the authors provide cogent discussions of the problems and pitfalls that may be encountered, as well as valuable troubleshooting advice. An appendix lists all companies whose products are cited in the text and includes an Internet directory for locating other reagent sources. Detailed and highly practical, SV40 Protocols offers both clinical and basic researchers powerful, well-tested tools for research on SV40 replication and neoplastic transformation, as well as techniques for its detection in human tumors and for creating and using powerful new gene therapy vectors.
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Includes bibliographical references and index.

Propagation of wild-type and mutant SV40 -- Propagation and assay of the JC virus -- Propagation and assay of BK virus -- Molecular methods for identification and genotyping of BK virus -- Methods for studying interactions between simian virus 40 T-antigen and the viral origin of replication -- Cell-free transcription of SV40 -- Quantitation and structural analysis of SV40 RNAs -- Generation of recombinant SV40 vectors for gene transfer -- Production of SV40 proteins in insect cells and in vitro packaging of virions and pseduovirions -- Using retroviral vectors to express SV40 tumor antigens -- Neoplastic transformation assays -- Methods to assess inhibition of adipocyte dfferentiation by SV40 large T antigen -- SV40-mediated immortalization -- Biology of p53 and SV40 large T association -- Binding of SV40 large T antigen to the retinoblastoma susceptibility gene product and related proteins -- In situ electroporation for the measurement of c-Ras activation by SVLT -- Role of SV40 small t in cell lysis, transformation, and signaling -- Cytotoxic T lymphocytes in SV40 infections -- Detection of SV40 DNA sequences in human tissue -- Guide to techniques in creating transgenic mouse models using SV40 T antigen.

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Extensive research has shown that Simian Virus 40, a contaminant of polio and adenovirus vaccines that may be implicated in human cancers, can also serve as a powerful probe for examining many fundamental questions in molecular biology. In SV40 Protocols, Leda Raptis and a panel of highly experienced investigators describe in step-by-step fashion key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and its related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for the use of retroviral vectors to express SV40 tumor antigens in cultured cells, and for transgenic mouse models based on the SV40 large T antigen. All methods have been optimized for experimental success, and the authors provide cogent discussions of the problems and pitfalls that may be encountered, as well as valuable troubleshooting advice. An appendix lists all companies whose products are cited in the text and includes an Internet directory for locating other reagent sources. Detailed and highly practical, SV40 Protocols offers both clinical and basic researchers powerful, well-tested tools for research on SV40 replication and neoplastic transformation, as well as techniques for its detection in human tumors and for creating and using powerful new gene therapy vectors.

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