Tissue engineering methods and protocols / edited by Jeffrey R. Morgan and Martin L. Yarmush.

Contributor(s): Morgan, Jeffrey Robert | Yarmush, Martin L
Material type: TextTextSeries: Methods in molecular medicine: 18.Publisher: Totowa, N.J. : Humana Press, ©1999Description: 1 online resource (xvi, 629 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592596027; 0896035166; 9780896035164; 1592596029Subject(s): Animal cell biotechnology | Tissue engineering | Tissue culture | Polymers in medicine | Cell Transplantation | Genetic Engineering | Regeneration | Biomedical Engineering | Culture Techniques | Methods | Cell Culture Techniques | Genetic Techniques | Investigative Techniques | Engineering | Transplantation | Biological Phenomena | Health Occupations | Clinical Laboratory Techniques | Surgical Procedures, Operative | Disciplines and Occupations | Technology, Industry, and Agriculture | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Phenomena and Processes | Technology, Industry, Agriculture | Animal cell biotechnology | Polymers in medicine | Tissue culture | Tissue engineeringGenre/Form: Electronic books. Additional physical formats: Tissue engineering methods and protocolsDDC classification: 571.5/381 LOC classification: TP248.27.A53 | T57 1999Online resources: Click here to access online
Contents:
Preparation of collagen-glycosaminoglycan copolymers for tissue regeneration / L.J. Chamberlain and I.V. Yannas-Preparation and use of tethered ligands as biomaterials and tools for cell biology / S.J. Sofia, P.R. Kuhl and L.G. Griffith-Nondestructive evaluation of biodegradable porous matrices for tissue engineering / L. Garrido-Fabrication of biodegradable polymer foams for cell transplantation and tissue engineering / P.X. Ma and R. Langer-Formation of highly porous polymeric foams with controlled release capability : A phase-separation technique / S. Kadiyala, H. Lo and K.W. Leong-Magnetic-induced alignment of collagen fibrils in tissue equivalents / T.S. Girton, N. Dubey and R.T. Tranquillo-Preparation and use of thermosensitive polymers / Y.L. Cao, C. Ibarra and C. Vacanti-In situ photopolymerized hydrogels for vascular and peritoneal wound healing / A.S. Sawhney and J.A. Hubbell-Fabrication and implantation of gel-filled nerve guidance channels / R.V. Bellamkonda and R.F. Valentini-Small animal surgical and histological procedures for characterizing the performance of tissue-engineered bone grafts / K.S. James, M.C. Zimmerman and J. Kohn-Preparation and use of porous poly(alpha-hydroxyester) scaffolds for bone tissue engineering / A.C. Jen, S.J. Peter and A.G. Mikos-Quantitative assessment of autocrine cell loops / G. Oehrtman [and others]-Measurement of recovery of function following whole muscle transfer, myoblast transfer, and gene therapy / J.A. Faulkner, S.V. Brooks and R.G. Dennis-Preparation of immortalized human chondrocyte cell lines / J.R. Robbins and M.B. Goldring-Isolation and in vitro proliferation of chondrocytes, tenocytes, and ligament cells / S. Shortkroff and M. Spector-Culture and identification of autologous human articular chondrocytes for implantation / R. Tubo and F. Binette-Organogenesis of skeletal muscle in tissue culture / H. Vandenburgh [and others]-Hepatocyte primary cultures : Currently used systems and their applications for studies of hepatocyte growth and differentiation / G.K. Michalopoulos-Formation and characterization of hepatocyte spheroids / J.R. Friend [and others]-Isolation and culture of human endothelial cells / F. Berthiaume-Isolation and culture of microvascular endothelial cells / L. Richard, P. Velasco and M. Detmar-Initiation, maintenance, and quantification of human hematopoietic cell cultures / P.C. Collins [and others]-Methods to isolate, culture, and study osteoblasts / M.V. Hillsley-Cryopreservation of rat hepatocytes in a three-dimensional culture configuration using a controlled-rate freezing device / M.J. Russo and M. Toner-Microencapsulation of enzymes, cells, and genetically engineered microorganisms / T.M. Chang-Methods for microencapsulation with hema-mma / S. Lahooti and M.V. Sefton-Micropatterning cells in tissue engineering / S.N. Bhatia, M.L. Yarmush and M. Toner-Methods for the serum-free culture of keratinocytes and transplantation of collagen-gag-based skin substitutes / S.T. Boyce-Use of skin equivalent technology in a wound healing model / M.A. Vaccariello [and others]-Preparation and transplantation of a composite graft of epidermal keratinocytes on acellular dermis / D.A. Medalie and J.R. Morgan-Development of a bioartificial liver device / L.K. Hansen [and others]-Methods for the implantation of liver cells / S.S. Kim, H. Utsunomiya and J.P. Vacanti-Isolation and long-term maintenance of adult rat hepatocytes in culture / F. Berthiaume, R.G. Tompkins and M.L. Yarmush-Design and fabrication of a small caliber hybrid arterial bioprosthesis / J. L'Ltalien G and W.M. Abbott-Methods for the immunoisolation and transplantation of pancreatic cells / A.M. Sun-Methods for the study of nerve cell migration and patterning / H.M. Buettner and H.C. Tai-Estimating number and volume of islets transplanted within a planar immunobarrier diffusion chamber / K. Suzuki [and others]-Quantitative measurement of cell-cell adhesion under flow conditions / C.L. Ramos and M.B. Lawrence-Quantitative measurement of the biological response of cartilage to mechanical deformation / R.G. Allen, S.R. Eisenberg and M.L. Gray-Measuring receptor-mediated cell adhesion under flow : Cell-free systems / D.A. Hammer and D.K. Brunk-In vitro and in vivo quantification of adhesion between leukocytes and vascular endothelium / R.K. Jain [and others]-Quantitative measurement of shear-stress effects on endothelial cells / M. Papadaki and L.V. McLntire-Quantitative modeling of limitations caused by diffusion / A. Sambanis and S.A. Tan.
Summary: Jeffrey Morgan and Martin Yarmush bring together in Tissue Engineering the first major collection of cutting-edge methods developed and used by leading researchers in the tissue engineering field. These hands-on experts describe easily reproducible protocols for the production and evaluation of polymers, scaffolds, and composites, as well as methods for the isolation, culture, and analysis of cells, including the combination of cells with a variety of materials or devices. Quantitative methods designed to assess cell performance and cell function that will also illuminate our understanding of cellular processes and the cellular response to the chemical and mechanical environment of a functional organ/tissue are also offered. Tissue Engineering provides researchers with detailed methods and protocols covering a comprehensive range of key technologies and techniques used by leaders in tissue engineering research. Its interdisciplinary methods-drawn from the life sciences, engineering, and clinical medicine-are already making a significant contribution to the development of new and effective treatments for both organ and tissue defects.
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Includes bibliographical references and index.

Preparation of collagen-glycosaminoglycan copolymers for tissue regeneration / L.J. Chamberlain and I.V. Yannas-Preparation and use of tethered ligands as biomaterials and tools for cell biology / S.J. Sofia, P.R. Kuhl and L.G. Griffith-Nondestructive evaluation of biodegradable porous matrices for tissue engineering / L. Garrido-Fabrication of biodegradable polymer foams for cell transplantation and tissue engineering / P.X. Ma and R. Langer-Formation of highly porous polymeric foams with controlled release capability : A phase-separation technique / S. Kadiyala, H. Lo and K.W. Leong-Magnetic-induced alignment of collagen fibrils in tissue equivalents / T.S. Girton, N. Dubey and R.T. Tranquillo-Preparation and use of thermosensitive polymers / Y.L. Cao, C. Ibarra and C. Vacanti-In situ photopolymerized hydrogels for vascular and peritoneal wound healing / A.S. Sawhney and J.A. Hubbell-Fabrication and implantation of gel-filled nerve guidance channels / R.V. Bellamkonda and R.F. Valentini-Small animal surgical and histological procedures for characterizing the performance of tissue-engineered bone grafts / K.S. James, M.C. Zimmerman and J. Kohn-Preparation and use of porous poly(alpha-hydroxyester) scaffolds for bone tissue engineering / A.C. Jen, S.J. Peter and A.G. Mikos-Quantitative assessment of autocrine cell loops / G. Oehrtman [and others]-Measurement of recovery of function following whole muscle transfer, myoblast transfer, and gene therapy / J.A. Faulkner, S.V. Brooks and R.G. Dennis-Preparation of immortalized human chondrocyte cell lines / J.R. Robbins and M.B. Goldring-Isolation and in vitro proliferation of chondrocytes, tenocytes, and ligament cells / S. Shortkroff and M. Spector-Culture and identification of autologous human articular chondrocytes for implantation / R. Tubo and F. Binette-Organogenesis of skeletal muscle in tissue culture / H. Vandenburgh [and others]-Hepatocyte primary cultures : Currently used systems and their applications for studies of hepatocyte growth and differentiation / G.K. Michalopoulos-Formation and characterization of hepatocyte spheroids / J.R. Friend [and others]-Isolation and culture of human endothelial cells / F. Berthiaume-Isolation and culture of microvascular endothelial cells / L. Richard, P. Velasco and M. Detmar-Initiation, maintenance, and quantification of human hematopoietic cell cultures / P.C. Collins [and others]-Methods to isolate, culture, and study osteoblasts / M.V. Hillsley-Cryopreservation of rat hepatocytes in a three-dimensional culture configuration using a controlled-rate freezing device / M.J. Russo and M. Toner-Microencapsulation of enzymes, cells, and genetically engineered microorganisms / T.M. Chang-Methods for microencapsulation with hema-mma / S. Lahooti and M.V. Sefton-Micropatterning cells in tissue engineering / S.N. Bhatia, M.L. Yarmush and M. Toner-Methods for the serum-free culture of keratinocytes and transplantation of collagen-gag-based skin substitutes / S.T. Boyce-Use of skin equivalent technology in a wound healing model / M.A. Vaccariello [and others]-Preparation and transplantation of a composite graft of epidermal keratinocytes on acellular dermis / D.A. Medalie and J.R. Morgan-Development of a bioartificial liver device / L.K. Hansen [and others]-Methods for the implantation of liver cells / S.S. Kim, H. Utsunomiya and J.P. Vacanti-Isolation and long-term maintenance of adult rat hepatocytes in culture / F. Berthiaume, R.G. Tompkins and M.L. Yarmush-Design and fabrication of a small caliber hybrid arterial bioprosthesis / J. L'Ltalien G and W.M. Abbott-Methods for the immunoisolation and transplantation of pancreatic cells / A.M. Sun-Methods for the study of nerve cell migration and patterning / H.M. Buettner and H.C. Tai-Estimating number and volume of islets transplanted within a planar immunobarrier diffusion chamber / K. Suzuki [and others]-Quantitative measurement of cell-cell adhesion under flow conditions / C.L. Ramos and M.B. Lawrence-Quantitative measurement of the biological response of cartilage to mechanical deformation / R.G. Allen, S.R. Eisenberg and M.L. Gray-Measuring receptor-mediated cell adhesion under flow : Cell-free systems / D.A. Hammer and D.K. Brunk-In vitro and in vivo quantification of adhesion between leukocytes and vascular endothelium / R.K. Jain [and others]-Quantitative measurement of shear-stress effects on endothelial cells / M. Papadaki and L.V. McLntire-Quantitative modeling of limitations caused by diffusion / A. Sambanis and S.A. Tan.

English.

Jeffrey Morgan and Martin Yarmush bring together in Tissue Engineering the first major collection of cutting-edge methods developed and used by leading researchers in the tissue engineering field. These hands-on experts describe easily reproducible protocols for the production and evaluation of polymers, scaffolds, and composites, as well as methods for the isolation, culture, and analysis of cells, including the combination of cells with a variety of materials or devices. Quantitative methods designed to assess cell performance and cell function that will also illuminate our understanding of cellular processes and the cellular response to the chemical and mechanical environment of a functional organ/tissue are also offered. Tissue Engineering provides researchers with detailed methods and protocols covering a comprehensive range of key technologies and techniques used by leaders in tissue engineering research. Its interdisciplinary methods-drawn from the life sciences, engineering, and clinical medicine-are already making a significant contribution to the development of new and effective treatments for both organ and tissue defects.

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