Laser capture microdissection : methods and protocols / edited by Graeme I. Murray and Stephanie Curran.

Contributor(s): Murray, Graeme I | Curran, Stephanie
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 293.Publisher: Totowa, N.J. : Humana Press, ©2005Description: 1 online resource (xiv, 319 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1588292606; 9781588292605; 1592598536; 9781592598533Subject(s): Molecular biology -- Methodology | Microdissection | Lasers | Lasers | Microdissection -- methods | Genetic Techniques | SCIENCE -- Life Sciences -- Molecular Biology | Lasers | Microdissection | Molecular biology -- MethodologyGenre/Form: Electronic books. Additional physical formats: Print version:: Laser capture microdissection.DDC classification: 572.8/028 LOC classification: QH506 | .L25 2005Online resources: Click here to access online
Contents:
An introduction to laser-based tissue microdissection techniques / Stephanie Curran and Graeme I. Murray -- Methacarn fixation for genomic DNA analysis in microdissected cells / Makoto Shibutani and Chikako Uneyama -- Multiplex quantitative real-time pcr of laser microdissected tissue / Patrick H. Rooney -- Comparative genomic hybridization using DNA from laser capture microdissected tissue / Grace Callagy, Lucy Jackson, and Carlos Caldas -- Detection of Ki-ras and p53 mutations by laser capture microdissection/PCR/SSCP / Deborah Dillon [and others] -- Whole-genome allelotyping using laser microdissected tissue / Colleen M. Feltmate and Samuel C. Mok -- Microdissection for detecting genetic aberrations in early and advanced human urinary bladder cancer / Arndt Hartmann [and others] -- Laser microdissection for microsatellite analysis in colon and breast cancer / Peter J. Wild [and others] -- Assessment of RET/PTC oncogene activation in thyroid nodules utilizing laser microdissection followed by nested RT-PCR / Giovanni Tallini and Guilherme Brandao -- Combined laser-assisted microdissection and short tandem repeat analysis for detection of in situ microchimerism after solid organ transplantation / Ulrich Lehmann, Anne Versmold, and Hans Kreipe -- Laser-assisted microdissection of membrane-mounted tissue sections / Lise Mette Gjerdrum and Stephen Hamilton-Dutoit -- Laser-assisted microdissection of membrane-mounted sections following immunohistochemistry and in situ hybridization / Lise Mette Gjerdrum and Stephen Hamilton-Dutoit -- Laser-assisted cell microdissection using the palm system / Patrick Micke [and others] -- Laser microdissection and RNA analysis / Ludger Fink and Rainer Maria Bohle -- Gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification, and genechip microarrays / Veronica I. Luzzi, Victoria Holtschlag, and Mark A. Watson -- Quantification of gene expression in mouse and human renal proximal tubules / Jun-ya Kaimori, Masaru Takenaka, and Kousaku Okubo -- Laser capture microdissection for analysis of macrophage gene expression from atherosclerotic lesions / Eugene Trogan and Edward A. Fisher -- Analysis of pituitary cells by laser capture microdissection / Ricardo V. Lloyd [and others] -- Laser capture microdissection and colorectal cancer proteomics / Laura C. Lawrie and Stephanie Curran -- Proteomic analysis of human bladder tissue using seldi approach following -- Microdissection techniques / Rene C. Krieg and others] -- Genetic analysis of HIV by in situ PCR-directed laser capture microscopy of infected -- Cells / Daniele Marras -- Use of laser capture microdissection together with in situ hybridization and real-time PCR to study the distribution of latent herpes simplex virus genomes in mouse trigeminal ganglion / Xiao-Ping Chen, Marina Mata, and David J. Fink -- Laser capture microdissection and PCR for analysis of human papilloma virus infection / Kheng Chew [and others] -- Laser capture microdissection of hepatic stages of the human parasite plasmodium falciparum for molecular analysis / Jean-Philippe Semblat [and others].
Summary: Use of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies.
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Includes bibliographical references and index.

An introduction to laser-based tissue microdissection techniques / Stephanie Curran and Graeme I. Murray -- Methacarn fixation for genomic DNA analysis in microdissected cells / Makoto Shibutani and Chikako Uneyama -- Multiplex quantitative real-time pcr of laser microdissected tissue / Patrick H. Rooney -- Comparative genomic hybridization using DNA from laser capture microdissected tissue / Grace Callagy, Lucy Jackson, and Carlos Caldas -- Detection of Ki-ras and p53 mutations by laser capture microdissection/PCR/SSCP / Deborah Dillon [and others] -- Whole-genome allelotyping using laser microdissected tissue / Colleen M. Feltmate and Samuel C. Mok -- Microdissection for detecting genetic aberrations in early and advanced human urinary bladder cancer / Arndt Hartmann [and others] -- Laser microdissection for microsatellite analysis in colon and breast cancer / Peter J. Wild [and others] -- Assessment of RET/PTC oncogene activation in thyroid nodules utilizing laser microdissection followed by nested RT-PCR / Giovanni Tallini and Guilherme Brandao -- Combined laser-assisted microdissection and short tandem repeat analysis for detection of in situ microchimerism after solid organ transplantation / Ulrich Lehmann, Anne Versmold, and Hans Kreipe -- Laser-assisted microdissection of membrane-mounted tissue sections / Lise Mette Gjerdrum and Stephen Hamilton-Dutoit -- Laser-assisted microdissection of membrane-mounted sections following immunohistochemistry and in situ hybridization / Lise Mette Gjerdrum and Stephen Hamilton-Dutoit -- Laser-assisted cell microdissection using the palm system / Patrick Micke [and others] -- Laser microdissection and RNA analysis / Ludger Fink and Rainer Maria Bohle -- Gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification, and genechip microarrays / Veronica I. Luzzi, Victoria Holtschlag, and Mark A. Watson -- Quantification of gene expression in mouse and human renal proximal tubules / Jun-ya Kaimori, Masaru Takenaka, and Kousaku Okubo -- Laser capture microdissection for analysis of macrophage gene expression from atherosclerotic lesions / Eugene Trogan and Edward A. Fisher -- Analysis of pituitary cells by laser capture microdissection / Ricardo V. Lloyd [and others] -- Laser capture microdissection and colorectal cancer proteomics / Laura C. Lawrie and Stephanie Curran -- Proteomic analysis of human bladder tissue using seldi approach following -- Microdissection techniques / Rene C. Krieg and others] -- Genetic analysis of HIV by in situ PCR-directed laser capture microscopy of infected -- Cells / Daniele Marras -- Use of laser capture microdissection together with in situ hybridization and real-time PCR to study the distribution of latent herpes simplex virus genomes in mouse trigeminal ganglion / Xiao-Ping Chen, Marina Mata, and David J. Fink -- Laser capture microdissection and PCR for analysis of human papilloma virus infection / Kheng Chew [and others] -- Laser capture microdissection of hepatic stages of the human parasite plasmodium falciparum for molecular analysis / Jean-Philippe Semblat [and others].

Print version record.

Use of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies.

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