Peptide synthesis and applications / edited by John Howl.

Contributor(s): Howl, John
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 298.Publisher: Totowa, N.J. : Humana Press, 2005Description: 1 online resource (x, 262 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1588293173; 9781588293176; 1592598773; 9781592598779Subject(s): Peptides -- Synthesis -- Laboratory manuals | Peptide Synthesis | SCIENCE -- Chemistry -- Organic | Peptides -- Synthesis | Biochemistry | Chemistry | Physical Sciences & MathematicsGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Peptide synthesis and applications.DDC classification: 547.7/56 LOC classification: QD431.25.S93 | P47 2005Online resources: Click here to access online
Contents:
Fundamentals of modern peptide synthesis / M. Amblard [and others] -- Chimerism: A strategy to expand the utility and applications of peptides / J. Howl -- Modification of peptides and other drugs using lipoamino acids and sugars / J.T. Blanchfield and I. Toth -- Synthesis of linear, branched, and cyclic peptide chimera / G. Mezo and F. Hudecz -- Synthesis of cell-penetrating peptides for cargo delivery / M. Pooga and U. Langel -- Incorporation of phosphotyrosyl mimetic 4-(phosphonodifluoromethyl)phenylalanine (F2Pmp) into signal transduction-directed peptides / Z.J. Yao, K. Lee and T.R. Burke, Jr. -- Expressed protein ligation for protein semisynthesis and engineering / Z. Machova and A.G. Beck-Sickinger -- Cellular delivery of peptide nucleic acid by cell-penetrating peptides / K. Kilk and U. Langel -- Quenched fluorescent substrate-based peptidase assays / R.A. Lew [and others] -- A convenient method for synthesis of cyclic peptide libraries / G.T. Bourne [and others] -- High-throughput peptide synthesis / M. Lebl and J. Hachmann -- Backbone amide linker strategies for the solid-phase synthesis of C-terminal modified peptides / J. Alsina [and others] -- Synthesis of peptide bioconjugates / F. Hudecz -- Protein identification by mass spectrometric analyses of peptides / A. Martin -- Manual solid-phase synthesis of glutathione analogs: A laboratory-based short course / U. Soomets, M. Zilmer and U. Langel.
Summary: Peptide synthesis provides novel materials that can serve as both valuable research tools and candidate therapeutics. In Peptide Synthesis and Applications, a panel of expert researchers and established group leaders describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate numerous applications that employ peptides as unique and essential materials. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Peptide Synthesis and Applications offers novice and experienced investigators alike powerful techniques for the design and manufacture of their own peptide-based materials that can be used as productive research tools or developed as promising new therapeutics.
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Includes bibliographical references and index.

Fundamentals of modern peptide synthesis / M. Amblard [and others] -- Chimerism: A strategy to expand the utility and applications of peptides / J. Howl -- Modification of peptides and other drugs using lipoamino acids and sugars / J.T. Blanchfield and I. Toth -- Synthesis of linear, branched, and cyclic peptide chimera / G. Mezo and F. Hudecz -- Synthesis of cell-penetrating peptides for cargo delivery / M. Pooga and U. Langel -- Incorporation of phosphotyrosyl mimetic 4-(phosphonodifluoromethyl)phenylalanine (F2Pmp) into signal transduction-directed peptides / Z.J. Yao, K. Lee and T.R. Burke, Jr. -- Expressed protein ligation for protein semisynthesis and engineering / Z. Machova and A.G. Beck-Sickinger -- Cellular delivery of peptide nucleic acid by cell-penetrating peptides / K. Kilk and U. Langel -- Quenched fluorescent substrate-based peptidase assays / R.A. Lew [and others] -- A convenient method for synthesis of cyclic peptide libraries / G.T. Bourne [and others] -- High-throughput peptide synthesis / M. Lebl and J. Hachmann -- Backbone amide linker strategies for the solid-phase synthesis of C-terminal modified peptides / J. Alsina [and others] -- Synthesis of peptide bioconjugates / F. Hudecz -- Protein identification by mass spectrometric analyses of peptides / A. Martin -- Manual solid-phase synthesis of glutathione analogs: A laboratory-based short course / U. Soomets, M. Zilmer and U. Langel.

Print version record.

Peptide synthesis provides novel materials that can serve as both valuable research tools and candidate therapeutics. In Peptide Synthesis and Applications, a panel of expert researchers and established group leaders describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate numerous applications that employ peptides as unique and essential materials. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Peptide Synthesis and Applications offers novice and experienced investigators alike powerful techniques for the design and manufacture of their own peptide-based materials that can be used as productive research tools or developed as promising new therapeutics.

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