Includes bibliographical references and index.

G1 and S-phase checkpoints, chromosome instability, and cancer / H. Nojima -- Analyzing the G2/M checkpoint / G.R. Stark and W.R. Taylor -- Analyzing the spindle checkpoint in yeast and frogs / P.T. Stukenberg and D.J. Burke -- Cell cycle checkpoint control mechanisms that can be disrupted in cancer / B.C. Dash and W.S. El-Deiry -- Establishment of a cell-free system to study the activation of chk2 / X. Xu and D.F. Stern -- Analyzing checkpoint controls in human skin / S. Pavey and B.G. Gabrielli -- Generation and analysis of brca1 conditional knockout mice / C.X. Deng and X. Xu -- Analysis of cell cycle progression and genomic integrity in early lethal knockouts / E.J. Brown -- Xenopus cell-free extracts to study the DNA damage response / V. Costanzo, K. Robertson and J. Gautier -- A Xenopus cell-free system for analysis of the chfr ubiquitin ligase involved in control of mitotic entry / D. Kang, J. Wong and G. Fang -- Control of mitotic entry after DNA damage in drosophila / B. Jaklevic, A. Purdy and T.T. Su -- Methods for analyzing checkpoint responses in caenorhabditis elegans / A. Gartner, A.J. MacQueen and A.M. Villeneuve -- Assaying the spindle checkpoint in the budding yeast saccharomyces cerevisiae / C.M. Yellman and D.J. Burke -- Purification and analysis of checkpoint protein complexes from saccharomyces cerevisiae / C.M. Green and N.F. Lowndes.

Print version record.

Intracellular checkpoint controls constitute a network of signal transduction pathways that protect cells from external stresses and internal errors by means of cell cycle arrest, DNA repair, or apoptosis. Failure of this machinery can lead to embryonic death, genetic diseases, and cancer. In Checkpoint Controls and Cancer, Volume 1: Reviews and Model Systems, Axel H. Schn̲thal, PhD, marshals a prestigious panel of researchers working at the cutting edges of their fields to comprehensively review the complexities of checkpoint controls and the model systems available to study them. The authors introduce all of the important components of checkpoint controls, describe their intricate interactions, and highlight the relevance of these processes to the cancer problem. Additional chapters illustrate the advantages of using such diverse model systems as intact human skin, knockout mice, Xenopus, and yeast, and show how they can cross-fertilize and accelerate research both across disciplines and beyond the boundaries of a particular species. A second volume, Activation and Regulation Protocols, provides readily reproducible experimental protocols for studying the molecular components of checkpoint controls and their regulation. Comprehensive and up-to-date, the two volumes of Checkpoint Controls and Cancer offer novice and experienced researchers alike not only entr ̌into the complexities of this vast field, but also to the full panoply of productive tools needed to deepen understanding of the systems, as well as to develop new and more effective cancer therapies.

English.