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Checkpoint controls and cancer. Volume 2, Activation and regulation protocols / edited by Axel H. Schönthal.

Contributor(s): Schönthal, Axel HMaterial type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 281.Publication details: Totowa, N.J. : Human Press, ©2004. Description: 1 online resource (1 volume) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592598113; 1592598110; 1588295001; 9781588295002; 1280359943; 9781280359941; 9786610359943; 6610359946Other title: Activation and regulation protocolsSubject(s): Carcinogenesis -- Laboratory manuals | Cellular signal transduction -- Laboratory manuals | Cellular control mechanisms -- Laboratory manuals | Neoplasms -- etiology | Signal Transduction | Cell Cycle Proteins -- physiology | Cell Cycle -- physiology | Laboratory Techniques and Procedures | Models, Biological | MEDICAL -- Oncology | HEALTH & FITNESS -- Diseases -- Cancer | Carcinogenesis | Cellular control mechanisms | Cellular signal transduction | neoplasms | celcyclus | cell cycle | moleculaire biologie | molecular biology | geneeskunde | medicine | biologische technieken | biological techniques | protocollen | protocols | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Checkpoint controls and cancer. Volume 2, Activation and regulation protocols.DDC classification: 616.99/4071 LOC classification: RC268.5 | .C48eb vol. 2NLM classification: W1 | QZ 202Online resources: Click here to access online
Contents:
Analysis of RB action in DNA damage checkpoint response / C.N. Mayhew [and others] -- Interaction between the retinoblastoma protein and protein phosphatase 1 during the cell cycle / N. Berndt and J.W. Ludlow -- Generation of p53 target database via integration of microarray and global p53 DNA-binding site analysis / S. Liu, A. Mirza and L. Wang -- Functional analysis of CDK inhibitor p21WAF1 / R. Fotedar, M. Bendjennat and A. Fotedar -- Analysis of p21CDKN1A recruitment to DNA excision repair foci in the UV-induced DNA damage response / L.A. Stivala and E. Prosperi -- Quantitative determination of p16 gene expression by RT-PCR / S. Schneider [and others] -- Measuring cyclin-dependent kinase activity / A.H. Schonthal -- Determination of the catalytic activities of mTOR and other members of the phosphoinositide-3-kinase-related kinase family / G.G. Chiang and R.T. Abraham -- CHK1 kinase activity assay / Y. Wang and H. Wang -- Assaying Cdc25 phosphatase activity / I. Hassepass and I. Hoffmann -- Analyzing the regulation and function of atm / M.F. Lavin [and others] -- Use of siRNA to study the function of MDC1 in DNA damage responses / Z. Lou and J. Chen -- Functional analysis of APC-Cdh1 / T. Sudo, N.T. Ueno and H. Saya -- Purification of the mitotic checkpoint complex, an inhibitor of the APC/C from HeLa cells / V. Sudakin and T.J. Yen -- Analysis of the spindle-assembly checkpoint in hela cells / P.R. Andreassen, D.A. Skoufias and R.L. Margolis -- Functional analysis of the spindle-checkpoint proteins using an in vitro ubiquitination assay / Z. Tang and H. Yu -- Analysis of checkpoint responses to histone deacetylase inhibitors / H. Beamish, R. Warrener and B.G. Gabrielli -- Biochemical analysis of the cell cycle and cell cycle checkpoints in transiently transfected cells after collection with magnetic beads / X. Ye [and others] -- Analysis of DNA repair and chromatin assembly in vitro using immobilized damaged DNA substrates / J.A. Mello, J.G. Moggs and G. Almouzni -- Analyzing cell cycle checkpoints after ionizing radiation / B. Xu and M.B. Kastan -- FACS-based detection of phosphorylated histone H3 for the quantitation of mitotic cells / W.R. Taylor -- Analysis of cell cycle by flow cytometry / P. Pozarowski and Z. Darzynkiewicz -- Analyzing markers of apoptosis in vitro / S. Plenchette [and others] -- Analysis of telomerase activity and telomere function in cancer / K.E. Gordon and E.K. Parkinson.
Summary: Intracellular checkpoint controls constitute a network of signal transduction pathways that protect cells from external stresses and internal errors by means of cell cycle arrest, DNA repair, or apoptosis. Failure of this machinery can lead to embryonic death, genetic diseases, and cancer. In Checkpoint Controls and Cancer, Volume 2: Activation and Regulation Protocols, Axel H. Schn̲thal, PhD, marshals a prestigious panel of researchers working at the cutting edges of their fields to present readily reproducible experimental protocols for the study of the molecular components of checkpoint controls and their regulation. Described in step-by-step detail, these powerful techniques offer such novel approaches as the use of genome databases and siRNA to analyze how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype. The earlier first volume, Reviews and Model Systems, comprehensively describes the complexities of checkpoint controls and the model systems available to study them. Comprehensive and up-to-date, the two volumes of Checkpoint Controls and Cancer offer novice and experienced researchers alike not only entr ̌into the complexities of this vast field, but also to the full panoply of productive tools needed to deepen understanding of the systems, as well as to develop new and more effective therapies.
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Includes bibliographical references and index.

Analysis of RB action in DNA damage checkpoint response / C.N. Mayhew [and others] -- Interaction between the retinoblastoma protein and protein phosphatase 1 during the cell cycle / N. Berndt and J.W. Ludlow -- Generation of p53 target database via integration of microarray and global p53 DNA-binding site analysis / S. Liu, A. Mirza and L. Wang -- Functional analysis of CDK inhibitor p21WAF1 / R. Fotedar, M. Bendjennat and A. Fotedar -- Analysis of p21CDKN1A recruitment to DNA excision repair foci in the UV-induced DNA damage response / L.A. Stivala and E. Prosperi -- Quantitative determination of p16 gene expression by RT-PCR / S. Schneider [and others] -- Measuring cyclin-dependent kinase activity / A.H. Schonthal -- Determination of the catalytic activities of mTOR and other members of the phosphoinositide-3-kinase-related kinase family / G.G. Chiang and R.T. Abraham -- CHK1 kinase activity assay / Y. Wang and H. Wang -- Assaying Cdc25 phosphatase activity / I. Hassepass and I. Hoffmann -- Analyzing the regulation and function of atm / M.F. Lavin [and others] -- Use of siRNA to study the function of MDC1 in DNA damage responses / Z. Lou and J. Chen -- Functional analysis of APC-Cdh1 / T. Sudo, N.T. Ueno and H. Saya -- Purification of the mitotic checkpoint complex, an inhibitor of the APC/C from HeLa cells / V. Sudakin and T.J. Yen -- Analysis of the spindle-assembly checkpoint in hela cells / P.R. Andreassen, D.A. Skoufias and R.L. Margolis -- Functional analysis of the spindle-checkpoint proteins using an in vitro ubiquitination assay / Z. Tang and H. Yu -- Analysis of checkpoint responses to histone deacetylase inhibitors / H. Beamish, R. Warrener and B.G. Gabrielli -- Biochemical analysis of the cell cycle and cell cycle checkpoints in transiently transfected cells after collection with magnetic beads / X. Ye [and others] -- Analysis of DNA repair and chromatin assembly in vitro using immobilized damaged DNA substrates / J.A. Mello, J.G. Moggs and G. Almouzni -- Analyzing cell cycle checkpoints after ionizing radiation / B. Xu and M.B. Kastan -- FACS-based detection of phosphorylated histone H3 for the quantitation of mitotic cells / W.R. Taylor -- Analysis of cell cycle by flow cytometry / P. Pozarowski and Z. Darzynkiewicz -- Analyzing markers of apoptosis in vitro / S. Plenchette [and others] -- Analysis of telomerase activity and telomere function in cancer / K.E. Gordon and E.K. Parkinson.

Print version record.

Intracellular checkpoint controls constitute a network of signal transduction pathways that protect cells from external stresses and internal errors by means of cell cycle arrest, DNA repair, or apoptosis. Failure of this machinery can lead to embryonic death, genetic diseases, and cancer. In Checkpoint Controls and Cancer, Volume 2: Activation and Regulation Protocols, Axel H. Schn̲thal, PhD, marshals a prestigious panel of researchers working at the cutting edges of their fields to present readily reproducible experimental protocols for the study of the molecular components of checkpoint controls and their regulation. Described in step-by-step detail, these powerful techniques offer such novel approaches as the use of genome databases and siRNA to analyze how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype. The earlier first volume, Reviews and Model Systems, comprehensively describes the complexities of checkpoint controls and the model systems available to study them. Comprehensive and up-to-date, the two volumes of Checkpoint Controls and Cancer offer novice and experienced researchers alike not only entr ̌into the complexities of this vast field, but also to the full panoply of productive tools needed to deepen understanding of the systems, as well as to develop new and more effective therapies.

English.

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